scholarly journals Neuronal activity patterns regulate BDNF expression in cortical neurons via synaptic connections and calcium signaling

2021 ◽  
Author(s):  
Yumi Miyasaka ◽  
Nobuhiko Yamamoto
Keyword(s):  
Calcium Signaling ◽  
Neuronal Activity ◽  
Cortical Neurons ◽  
Promoter Activity ◽  
Time Course ◽  
Activity Patterns ◽  
Cortical Circuits ◽  
Bdnf Expression ◽  
Intracellular Calcium Signaling ◽  
Sensory Evoked

AbstractDuring development, cortical circuits are remodeled by spontaneous and sensory-evoked activity via alteration of the expression of wiring molecules. An intriguing question is how physiological neuronal activity modifies the expression of these molecules in developing cortical networks. Here, we addressed this issue, focusing on brain-derived neurotrophic factor (BDNF), one of the factors underlying cortical wiring. Real-time imaging of Bdnf promoter activity in organotypic slice cultures revealed that patterned stimuli differentially regulated the increase and the time course of the promoter activity in upper layer neurons. Calcium imaging further demonstrated that stimulus-dependent increases in the promoter activity were roughly proportional to the increase in intracellular Ca2+ concentration per unit time. Finally, optogenetic stimulation showed that the promoter activity was increased efficiently by patterned stimulation in defined cortical circuits. These results suggest that physiological stimulation patterns differentially tune activity-dependent gene expression in developing cortical neurons via cortical circuits, synaptic responses, and alteration of intracellular calcium signaling.

scholarly journals Neuronal Activity Patterns Regulate Brain-Derived Neurotrophic Factor Expression in Cortical Cells via Neuronal Circuits

2021 ◽  
Vol 15 ◽  
Author(s):  
Yumi Miyasaka ◽  
Nobuhiko Yamamoto
Keyword(s):  
Neuronal Activity ◽  
Neurotrophic Factor ◽  
Cortical Neurons ◽  
Promoter Activity ◽  
Time Course ◽  
Activity Patterns ◽  
Brain Derived Neurotrophic Factor ◽  
Cortical Circuits ◽  
Cortical Cells ◽  
Sensory Evoked

During development, cortical circuits are remodeled by spontaneous and sensory-evoked activity via alteration of the expression of wiring molecules. An intriguing question is how physiological neuronal activity modifies the expression of these molecules in developing cortical networks. Here, we addressed this issue, focusing on brain-derived neurotrophic factor (BDNF), one of the factors underlying cortical wiring. Real-time imaging of BDNF promoter activity in organotypic slice cultures revealed that patterned stimuli differentially regulated the increase and the time course of the promoter activity in upper layer neurons. Calcium imaging further demonstrated that stimulus-dependent increases in the promoter activity were roughly proportional to the increase in intracellular Ca2+ concentration per unit time. Finally, optogenetic stimulation showed that the promoter activity was increased efficiently by patterned stimulation in defined cortical circuits. These results suggest that physiological stimulation patterns differentially tune activity-dependent gene expression in developing cortical neurons via cortical circuits, synaptic responses, and alteration of intracellular calcium signaling.

scholarly journals Dendritic nonlinearities are tuned for efficient spike-based computations in cortical circuits

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Balázs B Ujfalussy ◽  
Judit K Makara ◽  
Tiago Branco ◽  
Máté Lengyel
Keyword(s):  
Cortical Neurons ◽  
Activity Patterns ◽  
Pyramidal Cells ◽  
Cortical Circuits ◽  
Functional Link ◽  
Synaptic Inputs ◽  
Two Photon ◽  
Highly Nonlinear ◽  
Efficient Integration

Cortical neurons integrate thousands of synaptic inputs in their dendrites in highly nonlinear ways. It is unknown how these dendritic nonlinearities in individual cells contribute to computations at the level of neural circuits. Here, we show that dendritic nonlinearities are critical for the efficient integration of synaptic inputs in circuits performing analog computations with spiking neurons. We developed a theory that formalizes how a neuron's dendritic nonlinearity that is optimal for integrating synaptic inputs depends on the statistics of its presynaptic activity patterns. Based on their in vivo preynaptic population statistics (firing rates, membrane potential fluctuations, and correlations due to ensemble dynamics), our theory accurately predicted the responses of two different types of cortical pyramidal cells to patterned stimulation by two-photon glutamate uncaging. These results reveal a new computational principle underlying dendritic integration in cortical neurons by suggesting a functional link between cellular and systems--level properties of cortical circuits.

scholarly journals Corticothalamic gating of population auditory thalamocortical transmission in mouse

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Baher A Ibrahim ◽  
Caitlin A Murphy ◽  
Georgiy Yudintsev ◽  
Yoshitaka Shinagawa ◽  
Matthew I Banks ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Activity Patterns ◽  
Thalamic Reticular Nucleus ◽  
Reticular Nucleus ◽  
Sensory Stimuli ◽  
Population Responses ◽  
Cortical Responses ◽  
Sensory Evoked ◽  
Corticothalamic Feedback

The mechanisms that govern thalamocortical transmission are poorly understood. Recent data have shown that sensory stimuli elicit activity in ensembles of cortical neurons that recapitulate stereotyped spontaneous activity patterns. Here, we elucidate a possible mechanism by which gating of patterned population cortical activity occurs. In this study, sensory-evoked all-or-none cortical population responses were observed in the mouse auditory cortex in vivo and similar stochastic cortical responses were observed in a colliculo-thalamocortical brain slice preparation. Cortical responses were associated with decreases in auditory thalamic synaptic inhibition and increases in thalamic synchrony. Silencing of corticothalamic neurons in layer 6 (but not layer 5) or the thalamic reticular nucleus linearized the cortical responses, suggesting that layer 6 corticothalamic feedback via the thalamic reticular nucleus was responsible for gating stochastic cortical population responses. These data implicate a corticothalamic-thalamic reticular nucleus circuit that modifies thalamic neuronal synchronization to recruit populations of cortical neurons for sensory representations.

Alterations in intracellular calcium signaling of lymphocytes after exhaustive exercise

2001 ◽  
pp. 242-248 ◽  
Author(s):  
FRANK CH. MOOREN ◽  
ANJA LECHTERMANN ◽  
ALBERT FROMME ◽  
LOTHAR THORWESTEN ◽  
KLAUS V??LKER
Keyword(s):  
Calcium Signaling ◽  
Intracellular Calcium ◽  
Exhaustive Exercise ◽  
Intracellular Calcium Signaling

scholarly journals Presenilins: A novel link between intracellular calcium signaling and lysosomal function?

2012 ◽  
Vol 198 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Ilya Bezprozvanny
Keyword(s):  
Calcium Signaling ◽  
Intracellular Calcium ◽  
Transmembrane Proteins ◽  
Catalytic Subunit ◽  
Familial Alzheimer’S Disease ◽  
Lysosomal Function ◽  
Cell Biol ◽  
Intracellular Calcium Signaling ◽  
Lysosomal Acidification ◽  
In Cells

Mutations in presenilins (PS), transmembrane proteins encoding the catalytic subunit of γ-secretase, result in familial Alzheimer’s disease (FAD). Several studies have identified lysosomal defects in cells lacking PS or expressing FAD-associated PS mutations, which have been previously attributed to a function for PS in lysosomal acidification. Now, in this issue, Coen et al. (2012. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201201076) provide a series of results that challenge this idea and propose instead that presenilins play a role in calcium-mediated lysosomal fusion.

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