FastTomo: A SerialEM Script for Collecting Electron Tomography Data
Keyword(s):
AbstractFastTomo is a SerialEM script for collecting tilted specimen images in transmission electron microscopes to be further used in tomographic reconstruction. It achieves a speedup over conventional tracking methods by minimizing the usage of off-target tracking shots, and instead applies proportional control to the specimen images. Movement in the Z coordinate is estimated prior to each tilt series in a separate calibration routine. Overall, this method is fast and reliable when the field of view is at least 1 um, and can tolerate minor errors in setting eucentric height. The implemented tilt series schemes include the unidirectional, bidirectional, and dose-symmetric schemes.
2020 ◽
2021 ◽
1978 ◽
Vol 36
(2)
◽
pp. 82-83
◽
1974 ◽
Vol 32
◽
pp. 568-569
1969 ◽
Vol 27
◽
pp. 170-171
1978 ◽
Vol 36
(1)
◽
pp. 290-291