Towards fast, routine blood sample quality evaluation by Probe Electrospray Ionization (PESI) metabolomics

AbstractPESI-MS enables with its greatly simplified handling and fast result delivery the application field for high-throughput use in routine settings. In health care and research, pre-analytical errors often remain undetected and disrupt diagnosis, treatment, clinical studies and biomarker validations incurring high costs. This proof-of-principle study investigates the suitability of PESI-MS for robust, routine sample quality evaluation.One of the most common pre-analytical quality issues in blood sampling are prolonged transportations times from bedside to laboratory promptly changing the metabolome. Here, human blood (n=50) was processed immediately or with a time delay of 3 h. The developed sample preparation method delivers ready-to-measure extracts in <8 min. PESI-MS spectra were measured in both ionization modes in 2 min from as little as 2 µl plasma allowing 3 replicate measurements. The mass spectra contained 1200 stable features covering a broad chemical space covering major metabolic classes (e.g. fatty acids, lysolipids, lipids). The time delay of 3 h was predictable by using 18 features with AUC > 0.95 with various machine learning and was robust against loss of single features.Our results serve as first proof of principle for the unique advantages of PESI-MS in sample quality assessments. The results pave the way towards a fully automated, cost-efficient, user-friendly, robust and fast quality assessment of human blood samples from minimal sample amounts.Graphical abstract

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Design and Proof of Principle of a Novel Drip Chamber

Journal of Medical Devices ◽

10.1115/1.4045690 ◽

2020 ◽

Vol 14 (2) ◽

Author(s):

Else F. de Ridder ◽

Max D. B. Hoeboer ◽

Anne F. D. M. van Dam ◽

Job van den Bosch

Keyword(s):

Health Care ◽

The Netherlands ◽

Ease Of Use ◽

Future Research ◽

Air Bubbles ◽

The Novel ◽

Test Results ◽

Experimental Setting ◽

Cost Efficient ◽

Proof Of Principle

Abstract More than a million times every year, an ambulance is deployed in the Netherlands. Of these instances, 87% of the patients will be administered intravenous (IV) fluids. In the current situation, the IV bag always needs to be held above the patient to function properly. This action requires an extra pair of hands, which is very inefficient and can result in the loss of precious time that could otherwise be directed at the patient. Besides needing an extra pair of hands, there is also a chance of air bubbles entering the patient through the drip feed. The novel drip chamber design proposed in this paper aims to solve these problems, as it enables the IV bag to be placed in any orientation and in any manner relative to the patient with a negligible number of air bubbles entering the drip feed. This novel drip chamber was tested in an experimental setting in different orientations and at different flowrates. From the test results, it can be concluded that at a clinical relevant flowrate, a negligible number of air bubbles were present within the IV system. Because of the ease of use of the novel drip chamber and the fact that it is fool-proof, cost-efficient, and shows promising test results, future research on several aspects could make this product a promising addition to health care.

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Digital adaptive control of a human blood pressure model containing time delay

10.1109/cdc.1982.268246 ◽

1982 ◽

Cited By ~ 2

Author(s):

R. Dwiggins ◽

H. Kaufman ◽

R. Roy

Keyword(s):

Blood Pressure ◽

Adaptive Control ◽

Time Delay ◽

Human Blood

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Proof of principle of an on-line digitizer with ±18 ppm repeatability and 1.2 μs real-time delay for power converters control loop

Journal of Instrumentation ◽

10.1088/1748-0221/12/09/p09002 ◽

2017 ◽

Vol 12 (09) ◽

pp. P09002-P09002

Author(s):

P. Arpaia ◽

C. Baccigalupi ◽

M.C. Bastos ◽

M. Martino

Keyword(s):

Time Delay ◽

Real Time ◽

Power Converters ◽

Control Loop ◽

On Line ◽

Proof Of Principle

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SNP Genotyping and ApoE Genotyping v1

10.17504/protocols.io.by9ypz7w ◽

2021 ◽

Author(s):

Huw Morris ◽

Manuela MX Tan ◽

Donald G Grosset ◽

Nigel M Williams

Keyword(s):

Parkinson’S Disease ◽

Quality Control ◽

Blood Sample ◽

Dna Extraction ◽

Study Design ◽

Human Blood ◽

Patient Data ◽

Snp Genotyping ◽

Sample Quality ◽

Human Blood Sample

This protocol details the steps for DNA extraction from a human blood sample, quality control, and SNP and APOE genotyping. The protocol has been adapted from the PRoBaND SNP Genotyping and ApoE Genotyping Protocol. The overall protocol for PRoBaND /Tracking Parkinson’s is published: Malek, N., Swallow, D. M. A., Grosset, K. A., Lawton, M. A., Marrinan, S. L., Lehn, A. C., Bresner, C., Bajaj, N., Barker, R. A., Ben-Shlomo, Y., Burn, D. J., Foltynie, T., Hardy, J., Morris, H. R., Williams, N. M., Wood, N., & Grosset, D. G. (2015). Tracking Parkinson’s: Study Design and Baseline Patient Data. Journal of Parkinson’s Disease, 5(4), 947–959. https://doi.org/10.3233/JPD-150662

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Work-Based Framework for Sample Quality Evaluation of Low Plasticity Soils

Journal of Geotechnical and Geoenvironmental Engineering ◽

10.1061/(asce)gt.1943-5606.0001941 ◽

2018 ◽

Vol 144 (10) ◽

pp. 04018074 ◽

Cited By ~ 4

Author(s):

Jason T. DeJong ◽

Christopher P. Krage ◽

Brian M. Albin ◽

Don J. DeGroot

Keyword(s):

Quality Evaluation ◽

Sample Quality

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Ex vivo electric power generation in human blood using an enzymatic fuel cell in a vein replica

RSC Advances ◽

10.1039/c6ra17122b ◽

2016 ◽

Vol 6 (74) ◽

pp. 70215-70220 ◽

Cited By ~ 16

Author(s):

Dmitry Pankratov ◽

Lars Ohlsson ◽

Petri Gudmundsson ◽

Sanela Halak ◽

Lennart Ljunggren ◽

...

Keyword(s):

Power Generation ◽

Fuel Cell ◽

Electric Power ◽

Human Blood ◽

Electricity Generation ◽

Ex Vivo ◽

Blood Stream ◽

Biofuel Cell ◽

Enzymatic Fuel Cell ◽

Proof Of Principle

Proof-of-principle demonstration of sustained electricity generation by a biofuel cell operating in an authentic human blood stream.

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A Fuzzy-based Two-stage Biometric Sample Quality Evaluation System

ICASSP 2019 - 2019 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP) ◽

10.1109/icassp.2019.8682844 ◽

2019 ◽

Author(s):

Tauheed Ahmed ◽

Monalisa Sarma ◽

Debasis Samanta

Keyword(s):

Evaluation System ◽

Quality Evaluation ◽

Two Stage ◽

Sample Quality ◽

Quality Evaluation System

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Data Quality Evaluation in Aerospace Tracking Mission

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.651-653.2138 ◽

2014 ◽

Vol 651-653 ◽

pp. 2138-2144

Author(s):

Hui Fen Duan ◽

Wei Sun ◽

Hua Wang

Keyword(s):

Time Delay ◽

Data Quality ◽

Real Time ◽

Information Quality ◽

Quality Evaluation ◽

Measurement Information ◽

Error Range ◽

Data Statistics ◽

Information Problem

Modeled on data quality evaluating methodology, a standardized real-time rule framework is proposed. The framework is accurate and reliable for estimating aerospace experiment tracking information. By converting all the rule evaluating results that consisted of time-delay, error, range and etc. to a uniform questionable data count format, the framework presents an algorithm that takes both tracking source and station into account based on questionable data statistics for quantified information quality evaluating. The quantitative quality evaluation of measurement information problem can be solved via the proposed framework.

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