scholarly journals Insect-specific viruses regulate vector competence in Aedes aegypti mosquitoes via expression of histone H4

2021 ◽  
Author(s):  
Roenick P. Olmo ◽  
Yaovi Mathias H. Todjro ◽  
Eric R. G. R. Aguiar ◽  
Joao Paulo P. de Almeida ◽  
Juliana N. Armache ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Molecular Basis ◽  
Vector Competence ◽  
Spatiotemporal Analysis ◽  
Host Factor ◽  
Mosquito Vectors ◽  
Histone H4 ◽  
African Cities ◽  
Aedes Mosquitoes

Aedes aegypti and Aedes albopictus are major mosquito vectors for arthropod-borne viruses (arboviruses) such as dengue (DENV) and Zika (ZIKV) viruses. Mosquitoes also carry insect-specific viruses (ISVs) that may affect the transmission of arboviruses. Here, we analyzed the global virome in urban Aedes mosquitoes and observed that two insect-specific viruses, Phasi Charoen-like virus (PCLV) and Humaita Tubiacanga virus (HTV), were the most prevalent in A. aegypti worldwide except for African cities, where transmission of arboviruses is low. Spatiotemporal analysis revealed that presence of HTV and PCLV led to a 200% increase in the chances of having DENV in wild mosquitoes. In the laboratory, we showed that HTV and PCLV prevented downregulation of histone H4, a previously unrecognized proviral host factor, and rendered mosquitoes more susceptible to DENV and ZIKV. Altogether, our data reveals a molecular basis for the regulation of A. aegypti vector competence by highly prevalent ISVs that may impact how we analyze the risk of arbovirus outbreaks.

A Simple PCR Assay for Discrimination of Dengue Vectors from Nsukka LGA, Nigeria

2020 ◽  
Vol 10 (1) ◽  
pp. 67-77
Author(s):  
Amos Watentena ◽  
Ikem Chris Okoye ◽  
Ikechukwu Eugene Onah ◽  
Onwude Cosmas Ogbonnaya ◽  
Emmanuel Ogudu
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Mosquito Species ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Mosquito Vector ◽  
Aedes Mosquitoes ◽  
Allele Specific ◽  
Arboviral Diseases ◽  
Dengue Vectors

Mosquitoes of Aedes species are vectors of several arboviral diseases which continue to be a major public health problem in Nigeria. This study among other things, morphologically identified Aedes mosquitoes collected from Nsukka LGA and used an allele specific PCR amplification for discrimination of dengue vectors. Larval sampling, BG-sentinel traps and modified human landing catches were used for mosquito sampling in two selected autonomous communities of Nsukka LGA (Nsukka and Obimo). A total of 124 Aedes mosquitoes consisting of five (5) different species were collected from April to June, 2019 in a cross-sectional study that covered 126 households, under 76 distinct geographical coordinates. Larvae was mainly collected from plastic containers 73% (n=224), metallic containers 14% (n=43), earthen pots 9% (n=29) and used car tyres 3% (n=9), reared to adult stage 69.35% (n=86), and all mosquitoes were identified using standard morphological keys. Five (5) Aedes mosquito species were captured; Aedes aegypti 83(66.94%), Aedes albopictus 33(26.61%), Aedes simpsoni (4.48%), Aedes luteocephalus (≤1%) and Aedes vittatus (≤1%). Nsukka autonomous community had higher species diversity than Obimo. Allele specific amplification confirmed dengue vectors, Aedes aegypti and Aedes albopictus species on a 2% agarose gel. Since the most recent re-emergence of arboviral diseases is closely associated with Aedes species, findings of this study, therefore, give further evidence about the presence of potential arboviral vectors in Nigeria and describe the role of a simple PCR in discriminating some. Further entomological studies should integrate PCR assays in mosquito vector surveillance.

scholarly journals Zika mosquito vectors: the jury is still out

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 2546 ◽  
Author(s):  
Walter S. Leal
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Culex Quinquefasciatus ◽  
Zika Virus ◽  
Field Data ◽  
Vector Competence ◽  
Sexual Transmission ◽  
Mosquito Vectors ◽  
Yellow Fever Mosquito ◽  
The Common

After a 40-year hiatus, the International Congress of Entomology (ICE 2016) convened in Orlando, Florida (September 25-30, 2016). One of the symposia at ICE 2016, the Zika Symposium, covered multiple aspects of the Zika epidemic, including epidemiology, sexual transmission, genetic tools for reducing transmission, and particularly vector competence. While there was a consensus among participants that the yellow fever mosquito, Aedes aegypti, is a vector of the Zika virus, there is growing evidence indicating that the range of mosquito vectors might be wider than anticipated. In particular, three independent groups from Canada, China, and Brazil presented and discussed laboratory and field data strongly suggesting that the southern house mosquito, Culex quinquefasciatus, also known as the common mosquito, is highly likely to be a vector in certain environments.

scholarly journals Risk of dengue in Central Africa: Vector competence studies with Aedes aegypti and Aedes albopictus (Diptera: Culicidae) populations and dengue 2 virus

2019 ◽  
Vol 13 (12) ◽  
pp. e0007985 ◽  
Author(s):  
Basile Kamgang ◽  
Marie Vazeille ◽  
Armel N. Tedjou ◽  
Theodel A. Wilson-Bahun ◽  
Aurélie P. Yougang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Vector Competence ◽  
Central Africa

scholarly journals Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus

2020 ◽  
Vol 14 (4) ◽  
pp. e0007518 ◽  
Author(s):  
Thiago Nunes Pereira ◽  
Fabiano Duarte Carvalho ◽  
Silvana Faria De Mendonça ◽  
Marcele Neves Rocha ◽  
Luciano Andrade Moreira
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Culex Quinquefasciatus ◽  
Vector Competence ◽  
Mayaro Virus

scholarly journals Vector competence of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) for DEN2-43 and New Guinea C virus strains of dengue 2 virus

Acta Tropica ◽  
2013 ◽  
Vol 128 (3) ◽  
pp. 566-570 ◽  
Author(s):  
Xiao-Xia Guo ◽  
Xiao-Juan Zhu ◽  
Chun-Xiao Li ◽  
Yan-De Dong ◽  
Ying-Mei Zhang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Vector Competence ◽  
New Guinea ◽  
Virus Strains

Dengue introduced by travellers, Australia

2018 ◽  
Vol 39 (2) ◽  
pp. 67
Author(s):  
Allison Imrie
Keyword(s):  
At Risk ◽  
Aedes Aegypti ◽  
Viral Infection ◽  
Aedes Albopictus ◽  
International Travel ◽  
Severe Dengue ◽  
Risk Of Infection ◽  
Mosquito Vectors ◽  
Tourist Destinations ◽  
Acute Viral Infection

Dengue is a mosquito-borne acute viral infection that can develop into a potentially lethal complication known as severe dengue. It is endemic in more than 100 tropical and subtropical countries where the mosquito vectors, predominantly Aedes aegypti and Aedes albopictus, are found. Non-immune travellers are at risk of infection and with the rise in international travel and the availability of cheap holiday packages to endemic countries, many of which are popular tourist destinations, there has been a significant increase in spread of dengue viruses.

Key breeding sites of Aedes mosquitoes in Huaylau village, Pakse city, Champasack province, Lao People Democratic Republic 2019

2021 ◽  
Vol 05 (02) ◽  
pp. 52-61
Author(s):  
Thi Thanh Huong Le ◽  
◽  
Quynh Nguyen ◽  
Saenthavisouk Vanhnasack ◽  
Sinh Nam Vu
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Aedes Albopictus ◽  
Statistical Significance ◽  
P Value ◽  
Epidemic Threshold ◽  
Cross Sectional ◽  
Breeding Sites ◽  
Aedes Mosquitoes ◽  
The Village

Objectives: The elimination of key breeding sites of dengue vector is applied worldwide to control the vector density under the critical epidemic threshold. This study aimed to identify key breeding sites of Aedes mosquitos and associated factors in Huaylau village, Pakse city, Champasack province in Laos in 2019. Methods: This was a cross-sectional study, combining of immature stage survey of Aedes mosquitoes in all water and discarded containers and the community’s knowledge and practices on the prevention of dengue fever in all 240 households in Huaylau village. Multivariate logistic regression was applied to identify factors associated with the absence of Aedes larvae and pupae in the village. Statistical significance was set at a p-value less than 0.05. Results: Among the 720 water and discarded containers surveyed, the study found 654 Aedes larvae and pupae; of which 94.6% were immature Aedes aegypti. Key breeding sites of Aedes aegypti included water jars (34.2%), plastic drums (30.7%), tanks < 500 litter (14.7%), and discarded containers (12.6%), while that of all Aedes albopictus was discarded containers. The community’s better practices on the prevention of dengue fever contributed to the increased odds of the absence of Aedes larvae ad pupae by 1.612 (95%CI: 1.218-2.134, p<0.01), and the cleanliness of the house were associated with the increased odds of 4.072 of the absent Aedes larvae and pupae in the participated households (95%CI: 1.589-10.434, p<0.01). Conclusions: Aedes aegypti mosquitoes in the Huaylau village laid their eggs in various breeding sites, including water containers and discarded containers while discarded containers were the only key breeding site of Aedes albopictus. Better practices on dengue prevention and better hygiene conditions of the houses contributed to the absence of Aedes larvae and pupae. Special attention should be paid to the clearance of all discarded containers and education of the community people to cover water containers in the village. Keywords: key breeding sites, Aedes, mosquito, pupae, larvae, Champasack province, Lao PDR

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