ABSTRACTBactofilins are polymer-forming cytoskeletal proteins that are widely conserved in bacteria. Members of this protein family have diverse functional roles such as orienting subcellular molecular processes, establishing cell polarity, and aiding in cell shape maintenance. Chlamydia species are obligate intracellular bacteria that undergo a developmental cycle alternating between an infectious, non-dividing EB and a non-infectious, dividing RB. As Chlamydia divides by a polarized division process, we hypothesized that BacACT may function to establish polarity in these unique bacteria. Using sequence alignment to the conserved bactofilin domain, we identified a bactofilin ortholog, BacACT, in the obligate intracellular pathogen Chlamydia trachomatis. Utilizing a combination of fusion constructs and high-resolution fluorescence microscopy, we determined that BacACT forms a dynamic, membrane-associated, ring-like structure in Chlamydia’s replicative RB form. Contrary to our hypothesis, this filamentous ring structure is distinct from the microbe’s cell division machinery and does not colocalize with septal peptidoglycan or MreB, the major organizer of the bacterium’s division complex. Bacterial two-hybrid assays demonstrated BacACT interacts homotypically but does not directly interact with proteins involved in cell division or peptidoglycan biosynthesis. To investigate the function of BacACT in chlamydial development, we constructed a conditional knockdown strain using a newly developed CRISPR interference system. We observed that reducing bacACT expression significantly impacted chlamydial cell size and morphology. Normal RB morphology was restored when an additional copy of BacACT was expressed in trans during knockdown. These data reveal a novel function for chlamydial bactofilin in maintaining cell shape in this obligate intracellular bacterium.IMPORTANCEChlamydia is an ancient, obligate intracellular bacterium with a unique biphasic developmental cycle. As a result of its evolution within the osmotically stable environment of a host cell, Chlamydia has lost its dependence on side-wall peptidoglycan, and maintains only a fraction of the components thought to be required for regulating bacterial cell size and division. As such, very little is known about how Chlamydia species carry out these critical processes in the absence of a stabilizing peptidoglycan layer. In the current study, we identify a novel cytoskeletal element, termed a bactofilin, that is critical for maintaining the morphology of the bacteria. Using state-of-the-art genetic techniques for this organism, we demonstrate that chlamydial bactofilin forms a dynamic ring structure independent of the microbe’s division machinery and that abrogating its expression level using CRISPR interference results in abnormal morphologic forms. These findings enhance our understanding of chlamydial biology and bactofilins more generally.