scholarly journals Erythropoietin and miRNA profiles during the menstrual cycle in relation to hematological and lipid biomarkers

2021 ◽  
Author(s):  
Helena HB Bergstrom ◽  
Carmel Heiland ◽  
Linda Bjorkhem-Bergman ◽  
Lena Ekstrom
Keyword(s):  
Cardiovascular Diseases ◽  
Menstrual Cycle ◽  
Hematological Parameters ◽  
Autologous Blood ◽  
Cholesterol Homeostasis ◽  
Lipid Biomarkers ◽  
Autologous Blood Transfusion ◽  
Menstrual Cycles ◽  
Micro Rnas ◽  
Regular Menses

Background: Circulatory micro RNAs (miRNA) have been discussed as complementary diagnostic markers in cardiovascular diseases, and in anti-doping testing. MiR-144 and miR-486 have been associated with cholesterol homeostasis and hematopoiesis, respectively. In addition, they have been suggested as putative biomarkers for autologous blood transfusion and erythropoietin (EPO) doping. The aim of the present study was to assess the variability of miR-144-3p/5p, miR-486-5p/3p and EPO during the menstrual cycle. Secondary aim was to study the correlations between miRNAs, EPO and hematological parameters and lipids. Methods: 13 healthy women with regular menses were followed with weekly blood sampling during two whole menstrual cycles. MiRNAs were analyzed using TaqMan and PCR followed by calculation of the relative expression for each miRNA using ddCT approach. Results: There was no menstrual cycle variability in miRNAs and EPO. MiRNA-144-3p was associated with HDL-C (rs=-0.34, p=0.036) and miRNA-486-5p with Hb (rs=0.32, p=0.046). EPO concentrations correlated to lymphocytes (rs=-0.062, p=0.0002)), Hb (rs= -0.42, p=0.0091), HDL-C (rs=0.36, p=0.030) and triglycerides (rs=-0.54, p=0.0006). Conclusions: The results of this study may increase the understanding of how miR486-5p and miR144-3p as well as EPO correlate to hematopoietic and lipid biomarkers.

Effectiveness of zona pellucida protein ZPB as an immunocontraceptive antigen

Reproduction ◽  
2000 ◽  
pp. 19-32 ◽  
Author(s):  
ML Martinez ◽  
JD Harris
Keyword(s):  
Menstrual Cycle ◽  
Recombinant Protein ◽  
Antibody Titre ◽  
Zona Pellucida ◽  
Protein A ◽  
Papio Cynocephalus ◽  
Cynomolgus Monkeys ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle

Immunization of female mammals with native zona pellucida (ZP) proteins is known to cause infertility. Since each human ZP protein is now available as a purified recombinant protein, is it possible to compare the immunocontraceptive potential of each ZP protein. A breeding study was conducted in cynomolgus monkeys (Macaca fasicularis) after immunization with recombinant human ZP (rhZP) proteins (ZPA, ZPB, ZPC) separately and in combinations. This study demonstrated that immunization with recombinant human ZPB (rhZPB) protein caused cynomolgus monkeys to become infertile for 9-35 months. A second study was conducted in baboons (Papio cynocephalus), which yielded a similar result. The baboons immunized with rhZPB became infertile for 9 to > 20 months. During the time of maximum antibody titre, some animals experienced disruption of the menstrual cycle, but eventually all of the animals resumed normal menstrual cycles. Control animals and animals immunized with other rhZP proteins all became pregnant before any of the rhZPB-treated animals. This is the first study in which a recombinant ZP protein has consistently induced infertility in a primate without permanent disruption of the normal menstrual cycle.

Autologous blood transfusion for kidney transplant recipients

2000 ◽  
Vol 32 (7) ◽  
pp. 1853-1854 ◽  
Author(s):  
T Chikaraishi ◽  
T Iwamoto ◽  
T Hoshino ◽  
K Makizumi ◽  
N Yanagisawa ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Kidney Transplant ◽  
Autologous Blood ◽  
Autologous Blood Transfusion ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients

Surgical Program for Craniosynostosis Using Three-Dimensional Solid Model, Combined With Autologous Blood Transfusion in a Japanese Craniofacial Unit

2001 ◽  
Vol 12 (5) ◽  
pp. 479-484 ◽  
Author(s):  
Tetsuji Uemura ◽  
Takashi Hayashi ◽  
Yoshihiko Furukawa ◽  
Nobuyuki Mitsukawa ◽  
Atsushige Yoshikawa ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Autologous Blood ◽  
Three Dimensional ◽  
Solid Model ◽  
Autologous Blood Transfusion

Autologous Blood Transfusion in Elective Cardiac Valve Operations

2005 ◽  
Vol 20 (6) ◽  
pp. 513-518 ◽  
Author(s):  
Chad E. Lewis ◽  
Loren F. Hiratzka ◽  
Scott E. Woods ◽  
Mary P. Hendy ◽  
Amy M. Engel
Keyword(s):  
Blood Transfusion ◽  
Autologous Blood ◽  
Cardiac Valve ◽  
Autologous Blood Transfusion

Does the menstrual cycle influence the sensitivity of vagally mediated baroreflexes?

2002 ◽  
Vol 102 (6) ◽  
pp. 639-644 ◽  
Author(s):  
William H. COOKE ◽  
David A. LUDWIG ◽  
Paul S. HOGG ◽  
Dwain L. ECKBERG ◽  
Victor A. CONVERTINO
Keyword(s):  
Menstrual Cycle ◽  
Young Women ◽  
Regulatory Mechanisms ◽  
Physiological Changes ◽  
Menstrual Cycles ◽  
Time Points ◽  
Normal Menstrual Cycle ◽  
Cardiac Regulation ◽  
Neck Pressure ◽  
Noradrenaline Levels

The menstrual cycle provokes several physiological changes that could influence autonomic regulatory mechanisms. We studied the carotid-cardiac baroreflex in ten healthy young women on four occasions over the course of their menstrual cycles (days 0-8, 9-14, 15-20 and 21-25). We drew blood during each session for analysis of oestrogen, progesterone and noradrenaline (norepinephrine) levels, and assessed carotid-cardiac baroreflex function by analysing R-R interval responses to graded neck pressure sequences. Oestrogen levels followed a classical two-peak (cubic) response, with elevated levels on days 9-14 and 21-25 compared with days 0-8 and 15-20 (P =0.0032), while progesterone levels increased exponentially from days 9-14 to days 21-25 (P = 0.0063). Noradrenaline levels increased from an average of 137pg/ml during the first three measurement periods to 199pg/ml during days 21-25 (P = 0.0456). Carotid-cardiac baroreflex gain and operational point were not statistically different at any of the time points during the menstrual cycle (P⩾0.18). These findings are consistent with the notion that beat-to-beat vagal-cardiac regulation does not change over the course of the normal menstrual cycle.

Predeposit autologous blood transfusion in patients with colorectal cancer: A feasibility study

1992 ◽  
Vol 79 (4) ◽  
pp. 355-357 ◽  
Author(s):  
S. Harrison ◽  
R. J. C. Steele ◽  
A. K. Johnston ◽  
J. A. Jones ◽  
D. L. Morris ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Blood Transfusion ◽  
Feasibility Study ◽  
Autologous Blood ◽  
Autologous Blood Transfusion

Acute Isovolaemic Haemodilution: The Best Option for Autologous Blood Transfusion in Africa?

1995 ◽  
Vol 25 (4) ◽  
pp. 152-155 ◽  
Author(s):  
Zacharia A Berege ◽  
Bart Jacobs ◽  
Michael R Matasha ◽  
Frank Mpelumbe ◽  
Ernestini Kimaro
Keyword(s):  
Blood Transfusion ◽  
Blood Loss ◽  
Autologous Blood ◽  
Autologous Blood Transfusion ◽  
Total Blood Volume ◽  
East African ◽  
Homologous Blood ◽  
Total Blood ◽  
Induction Of Anaesthesia ◽  
Donated Blood

The purpose of this study was to identify the best method of autologous blood transfusion to be applied in an East African hospital. One hundred and nine consecutive patients for whom major blood loss was anticipated were enrolled. Seventeen patients donated 1 unit of blood 3 days preoperatively and 92 underwent acute isovolaemic haemodilution prior to induction of anaesthesia. For the haemodiluted patients a 2:1 ratio of sterile pryogen-free saline to collected blood was used. One of the 16 patients from whom 2 units were withdrawn by haemodilution experienced hypovolaemia which was rapidly restored by additional transfusion of colloid. Of the patients who donated blood preoperatively only 23.5% were autotransfused compared to 98.9% of the haemodiluted patients. Of the latter 23.9% (22) had an intraoperative blood loss exceeding 15% of their total blood volume and 7.6% (7) lost more than 25%. Only one received homologous blood in addition. For hospitals with limited blood bank facilities and regular cancellation of surgery, the use of acute isovolaemic haemodilution is recommended. A 3:1 ratio of saline to blood is now advised when 1 unit is withdrawn and a part replacement with crystalloid when 2 units are collected.

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