Heart rate and startle responses in diving, captive harbour porpoises (Phocoena phocoena) exposed to transient noise and sonar

ABSTRACT Anthropogenic noise can alter marine mammal behaviour and physiology, but little is known about cetacean cardiovascular responses to exposures, despite evidence that acoustic stressors, such as naval sonars, may lead to decompression sickness. Here, we measured heart rate and movements of two trained harbour porpoises during controlled exposure to 6–9 kHz sonar-like sweeps and 40 kHz peak-frequency noise pulses, designed to evoke acoustic startle responses. The porpoises initially responded to the sonar sweep with intensified bradycardia despite unaltered behaviour/movement, but habituated rapidly to the stimuli. In contrast, 40 kHz noise pulses consistently evoked rapid muscle flinches (indicative of startles), but no behavioural or heart rate changes. We conclude that the autonomous startle response appears decoupled from, or overridden by, cardiac regulation in diving porpoises, whereas certain novel stimuli may motivate oxygen-conserving cardiovascular measures. Such responses to sound exposure may contribute to gas mismanagement for deeper-diving cetaceans.

Symbolic Analysis of the Heart Rate Variability During the Plateau Phase Following Maximal Sprint Exercise

Cardiac autonomic control is commonly assessed via the analysis of fluctuations of the temporal distance between two consecutive R-waves (RR). Cardiac regulation assessment following high intensity physical exercise is difficult due to RR non-stationarities. The very short epoch following maximal sprint exercise when RR remains close to its lowest value, i.e., the PLATEAU, provides the opportunity to evaluate cardiac regulation from stationary RR sequences. The aim of the study is to evaluate cardiac autonomic control during PLATEAU phase following 60-m maximal sprint and compare the results to those derived from sequences featuring the same length as the PLATEAU and derived from pre-exercise and post-exercise periods. These sequences were referred to as PRE and POST sequences. RR series were recorded in 21 subjects (age: 24.9 ± 5.1 years, 15 men and six women). We applied a symbolic approach due to its ability to deal with very short RR sequences. The symbolic approach classified patterns formed by three RRs according to the sign and number of RR variations. Symbolic markers were compared to more classical time and frequency domain indexes. Comparison was extended to simulated signals to explicitly evaluate the suitability of methods to deal with short variability series. A surrogate test was applied to check the null hypothesis of random fluctuations. Over simulated data symbolic analysis was able to separate dynamics with different spectral profiles provided that the frame length was longer than 10 cardiac beats. Over real data the surrogate test indicated the presence of determinism in PRE, PLATEAU, and POST sequences. We found that the rate of patterns with two variations with unlike sign increased during PLATEAU and in POST sequences and the frequency of patterns with no variations remained unchanged during PLATEAU and decreased in POST compared to PRE sequences. Results indicated a sustained sympathetic control along with an early vagal reactivation during PLATEAU and a shift of the sympathovagal balance toward vagal predominance in POST compared to PRE sequences. Time and frequency domains markers were less powerful because they were dominated by the dramatic decrease of RR variance during PLATEAU.

Nitric Oxide and S-Nitrosylation in Cardiac Regulation: G Protein-Coupled Receptor Kinase-2 and β-Arrestins as Targets

Cardiac diseases including heart failure (HF), are the leading cause of morbidity and mortality globally. Among the prominent characteristics of HF is the loss of β-adrenoceptor (AR)-mediated inotropic reserve. This is primarily due to the derangements in myocardial regulatory signaling proteins, G protein-coupled receptor (GPCR) kinases (GRKs) and β-arrestins (β-Arr) that modulate β-AR signal termination via receptor desensitization and downregulation. GRK2 and β-Arr2 activities are elevated in the heart after injury/stress and participate in HF through receptor inactivation. These GPCR regulators are modulated profoundly by nitric oxide (NO) produced by NO synthase (NOS) enzymes through S-nitrosylation due to receptor-coupled NO generation. S-nitrosylation, which is NO-mediated modification of protein cysteine residues to generate an S-nitrosothiol (SNO), mediates many effects of NO independently from its canonical guanylyl cyclase/cGMP/protein kinase G signaling. Herein, we review the knowledge on the NO system in the heart and S-nitrosylation-dependent modifications of myocardial GPCR signaling components GRKs and β-Arrs.

Comparison of effect of aquatic interventions on cardiac modulation of obese young males in motion. A crossover trial

Study aim: The study aimed to compare the effects of passive Watsu therapy and immersion on cardiac locomotor synchronization of obese young males. Material and methods: Twenty-six volunteer obese males participated in this study (age 18.3 ± 0.32, BMI 36.9 ± 6.52). Heart rate variability parameters were recorded in different positions by the Polar H7 heart rate sensor and HRV+ software. Participants were assigned to two groups, randomly, in a single-blinded crossover design. Kubios HRV 2.2 and MATLAB were used to analyze the bio-signals. Statistical analysis was performed via t-test and ANOVA (analysis of variance) using SPSS. For the significance in results and group comparison, the paired t-test and the independent t-test were used respectively. Results: Combined results indicated that Watsu therapy increased 3 HRV vertical position parameters and immersion increased 3 HRV non-locomotor parameters, significantly (p < 0.05). Conclusion: The findings show that Watsu and immersion improved the specific autonomic cardiac modulation. However, non-contact immersion seemed to provide better synchronization of cardiac control and locomotion. The close contact Watsu approach provided improvements in autonomic cardiac regulation. Collectively, these improvements suggest the combination of both therapies in maximizing the cardiac benefits sought by aquatic therapy programs.

INDICATORS OF HEART RATE VARIABILITY AT REST AND DURING AN ORTHOSTATIC CHALLENGE AT DIFFERENT RANGES OF MxDMn VALUE AND THEIR CHANGE IN TRACK AND FIELD ATHLETES IN THE TRAINING PROCESS

The aim of the study is to develop the standards of HRV indexes at rest and during an orthostatic challenge, taking into account different ranges of variability of cardiac intervals (MxDMn) of track and field athletes. The other purpose is to identify the features of changes in the standards in the training process in cases of malfunctions of cardioregulatory systems, sinus node functioning and recovery process, overtraining, and decrease in athletic performance. The authors used the results of individual dynamic express-tests of heart rate variability (HRV) in track and field athletes belonging to different specific training orientations to achieve the goals of the study. Research Methods and Organization. We carried out 1740 dynamic HRV measurements at rest and during an orthostatic challenge. The studies involved 56 track and field athletes (sprinters, middle distance runners and stayers) aged 18 to 29 years, belonging to the 1st adult category, Candidates Master of Sports and Masters of Sports, in different periods of the training process. We carried out HRV measurements in the laboratory of functional research methods of the Institute of physical culture and sports at Udmurt State University, as well as at training camps in the middle mountains (Kyrgyzstan, Kislovodsk) and on the plain (Elabuga). We examined the test subjects at rest in the morning after the previous training day, using the VARICARD 2.51 device and the ISCIM6 and VARICARD MP programs (Ryazan). We recorded cardio intervals of athletes for 5 minutes in lying position and for 6 minutes in standing position. We applied HRV measurements to one or four athletes simultaneously using the ISCIM6 and VARICARD MP programs. Research results. We assigned special emphasis to the assessment of changes in the variational range of cardiointervals (MxDMn) reflecting the state of cardiac regulation and the sinus node functioning during dynamic HRV studies in the training process of each runner. We identified seven MxDMn variation ranges from <150ms to >650ms. We revealed that each MxDMn variation range corresponds to a certain prevailing type of regulation. We demonstrated that frequent shifts of the MxDMn ranges from one level to another in the training process of runners indicate instability of cardiac regulation. We elaborated standards for the variational range of cardiointervals (MxDMn), taking into account the predominance of the HF and LF power in the HRV power spectrum for runners with different specific training orientations. We found that respiratory (HF) or vasomotor waves (LF) could prevail within the same MxDMn variation ranges, which points to a different autonomic balance. We revealed that at rest, the variational range of cardiointervals (MxDMn) depends more on the state of cardiac regulation and the sinus node functioning and less on the specifics of running. We often detected paradoxical reactions to an orthostatic challenge at low or extremely high MxDMn values in the overtrained runners. We determined that runners of any specific training orientation could increase their fitness, adaptive and reserve capabilities only in the context of the optimal regulation state, stable favorable ranges of MxDMn values, and the absence of paradoxical reactions to an orthostatic challenge. The paper contains tables with standard HRV indicators for different MxDMn variation ranges and consideration of the predominance of HF and LF waves at rest and during an orthostatic challenge, as well as examples of the results of HRV analysis at rest and during an orthostatic challenge in runners with different MxDMn variation ranges in the training process. Conclusion. The research demonstrated that the use of the HRV analysis method at rest and during an orthostatic challenge, taking into account the standards of MxDMn values in the training process of track and field athletes, is aimed at obtaining the necessary information about the stability of autonomic balance, autonomic reactivity and body reserves that ensure optimal adaptation and recovery processes.

Role of the autonomic nervous system in atrial fibrillation pathogenesis

The aim of this review was to study the role of the autonomic nervous system in the pathogenesis of atrial fibrillation (AF), as well as to establish the relationship of autonomic regulation with other mechanisms underlying the AF At present, the molecular and cellular mechanisms underlying the AF have not been precisely established. There is interest in evidence showing that both sympathetic outflow and an increased vagal tone can initiate and support AF. As modern studies have shown, autonomic cardiac regulation can be an important factor in the pathogenesis of AF.

Introducing BisQ, A Bicoherence-Based Nonlinear Index to Explore the Heart Rhythm

Nonlinear frequency coupling is assessed with bispectral measures, such as bicoherence. In this study, BisQ, a new bicoherence-derived index, is proposed for assessing nonlinear processes in cardiac regulation. To find BisQ, 110 ten-minute ECG traces obtained from 55 participants were initially studied. Via bispectral analysis, a bicoherence matrix (BC) was obtained from each trace (0.06 to 1.8 Hz with a resolution of 0.01 Hz). Each frequency pair in BC was tested for correlation with the HRV recurrent quantification analysis (RQA) index Lmean, obtained from tachograms from the same ECG trace. BisQ is the result of adding BC values corresponding to the three frequency pairs exhibiting the highest correlation with Lmean. BisQ values were estimated for different groups of subjects: healthy persons, persons with arrhythmia, persons with epilepsy, and preterm neonates. ECG traces from persons with arrhythmia showed no significant differences in BisQ values respect to healthy persons, while persons with epilepsy and neonates showed higher BisQ values (p < 0.05; Mann-Whitney U-test). BisQ reflects nonlinear interactions at the level of sinus-and atrial-ventricular nodes, and most likely cardiorespiratory coupling as well. We expect that BisQ will allow for further exploration of cardiac nonlinear dynamics, complementing available HRV indices.