scholarly journals Neural circuit basis of aversive odour processing in Drosophila from sensory input to descending output

2018 ◽  
Author(s):  
Paavo Huoviala ◽  
Michael-John Dolan ◽  
Fiona M. Love ◽  
Philip Myers ◽  
Shahar Frechter ◽  
...  
Keyword(s):  
Sensory Input ◽  
Neural Circuit ◽  
Cell Types ◽  
Second Order ◽  
Egg Laying ◽  
Descending Pathways ◽  
Third Order ◽  
Descending Neurons ◽  
Central Brain ◽  
Multiple Levels

AbstractEvolution has shaped nervous systems to produce stereotyped behavioural responses to ethologically relevant stimuli. For example when laying eggs, female Drosophila avoid geosmin, an odorant produced by toxic moulds. Here we identify second, third, and fourth order neurons required for this innate olfactory aversion. Connectomics data place these neurons in a complete synaptic circuit from sensory input to descending output. We find multiple levels of valence-specific convergence, including a novel form of axo-axonic input onto second order neurons conveying another danger signal, the pheromone of parasitoid wasps. However, we also observe extensive divergence: second order geosmin neurons connect with a diverse array of 80 third order cell types. We find a pattern of convergence of aversive odour channels at this level. Crossing one more synaptic layer, we identified descending neurons critical for egg-laying aversion. Our data suggest a transition from a labelled line organisation in the periphery to a highly distributed central brain representation that is then coupled to distinct descending pathways.

scholarly journals Cell types and neuronal circuitry underlying female aggression in Drosophila

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Catherine E Schretter ◽  
Yoshinori Aso ◽  
Alice A Robie ◽  
Marisa Dreher ◽  
Michael-John Dolan ◽  
...  
Keyword(s):  
Social Interactions ◽  
Visual Information ◽  
Internal State ◽  
Cell Types ◽  
Female Aggression ◽  
Sexually Dimorphic ◽  
Neuronal Circuitry ◽  
Genetic Inactivation ◽  
Descending Neurons ◽  
Central Brain

Aggressive social interactions are used to compete for limited resources and are regulated by complex sensory cues and the organism’s internal state. While both sexes exhibit aggression, its neuronal underpinnings are understudied in females. Here, we identify a population of sexually dimorphic aIPg neurons in the adult Drosophila melanogaster central brain whose optogenetic activation increased, and genetic inactivation reduced, female aggression. Analysis of GAL4 lines identified in an unbiased screen for increased female chasing behavior revealed the involvement of another sexually dimorphic neuron, pC1d, and implicated aIPg and pC1d neurons as core nodes regulating female aggression. Connectomic analysis demonstrated that aIPg neurons and pC1d are interconnected and suggest that aIPg neurons may exert part of their effect by gating the flow of visual information to descending neurons. Our work reveals important regulatory components of the neuronal circuitry that underlies female aggressive social interactions and provides tools for their manipulation.

Bulk Contributions Modulate the Sum-Frequency Generation Spectra of Interfacial Water on Model Sea-Spray Aerosols

2018 ◽  
Author(s):  
Sandeep K. Reddy ◽  
Raphael Thiraux ◽  
Bethany A. Wellen Rudd ◽  
Lu Lin ◽  
Tehseen Adel ◽  
...  
Keyword(s):  
Single Layer ◽  
Sum Frequency Generation ◽  
Second Order ◽  
Interfacial Structure ◽  
Sea Spray ◽  
Third Order ◽  
Systematic Analysis ◽  
Sum Frequency ◽  
Structure Of Water ◽  
Frequency Generation

Vibrational sum-frequency generation (vSFG) spectroscopy is used to determine the molecular structure of water at the interface of palmitic acid monolayers. Both measured and calculated spectra display speci c features due to third-order contributions to the vSFG response which are associated with nite interfacial electric potentials. We demonstrate that theoretical modeling enables to separate the third-order contributions, thus allowing for a systematic analysis of the strictly surface-sensitive, second-order component of the vSFG response. This study provides fundamental, molecular-level insights into the interfacial structure of water in a neutral surfactant system with relevance to single layer bio-membranes and environmentally relevant sea-spray aerosols. These results emphasize the key role that computer simulations can play in interpreting vSFG spectra and revealing microscopic details of water at complex interfaces, which can be difficult to extract from experiments due to the mixing of second-order, surface-sensitive and third-order, bulk-dependent contributions to the vSFG response.

Evidence for general base catalysis by protic solvents in a kinetic study of alcoholyses and hydrolyses of 1-(phenoxycarbonyl)pyridinium ions under both solvolytic and non-solvolytic conditions

2009 ◽  
Vol 74 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Dennis N. Kevill ◽  
Byoung-Chun Park ◽  
Jin Burm Kyong
Keyword(s):  
Second Order ◽  
Base Catalysis ◽  
Substitution Reactions ◽  
Third Order ◽  
Methoxy Derivative ◽  
First Order ◽  
General Base ◽  
Protic Solvents ◽  
Kinetics Of ◽  
Pyridinium Ions

The kinetics of nucleophilic substitution reactions of 1-(phenoxycarbonyl)pyridinium ions, prepared with the essentially non-nucleophilic/non-basic fluoroborate as the counterion, have been studied using up to 1.60 M methanol in acetonitrile as solvent and under solvolytic conditions in 2,2,2-trifluoroethan-1-ol (TFE) and its mixtures with water. Under the non- solvolytic conditions, the parent and three pyridine-ring-substituted derivatives were studied. Both second-order (first-order in methanol) and third-order (second-order in methanol) kinetic contributions were observed. In the solvolysis studies, since solvent ionizing power values were almost constant over the range of aqueous TFE studied, a Grunwald–Winstein equation treatment of the specific rates of solvolysis for the parent and the 4-methoxy derivative could be carried out in terms of variations in solvent nucleophilicity, and an appreciable sensitivity to changes in solvent nucleophilicity was found.

Panicle, spikelet, and floret development in orchardgrass (Dactylis glomerata)

1993 ◽  
Vol 71 (4) ◽  
pp. 523-532 ◽  
Author(s):  
Joanna Fraser ◽  
Eric G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Unique Feature ◽  
Dactylis Glomerata ◽  
Bilateral Symmetry ◽  
Second Order ◽  
Third Order ◽  
First Order ◽  
Lateral Branches ◽  
Scanning Electron

The initial stages of panicle, spikelet, and floret development in field-grown 'Kay' orchardgrass were examined using scanning electron microscopy. Spikelets arose from a complex multilevelled sequence of initiation from branch apices. Spikelets developed indirectly in a two-tiered progression: (i) an acropetal and basipetal sequence of first order, second-order, and third-order inflorescence apices, and (ii) an acropetal development within subclusters of higher-order lateral branch inflorescence apices. The panicle had the unique feature of dorsiventrality as well as bilateral symmetry. The basal apex from first-order, second-order, or third-order apices developed on the same side of the main axis as the first-order apex. The two glumes subtending each spikelet primordium developed alternately and acropetally. Development and initiation of florets within spikelets was basipetal within the panicle, basipetal within clusters and subclusters of spikelets on lateral branches, and acropetal within spikelets. Within florets, paleas developed later than lemmas. Key words: Dactylis glomerata, cocksfoot, scanning electron microscopy, development, panicle.

scholarly journals Probing the Locally Generated Even and Odd Order Nonlinearity in Y–Ba–Cu–O and Tl–Ba–Ca–Cu–O (2212) Microwave Resonators Around <img src="/images/tex/19586.gif" alt="T_{\rm C}">

2013 ◽  
Vol 23 (3) ◽  
pp. 9000105-9000105 ◽  
Author(s):  
Brooke Jeries ◽  
Sean Cratty ◽  
S Remillard
Keyword(s):  
Meissner Effect ◽  
Second Order ◽  
Intermodulation Distortion ◽  
Coaxial Cable ◽  
Superconducting Resonators ◽  
Third Order ◽  
Odd Order ◽  
Similar Temperature Dependence ◽  
Similar Temperature ◽  
Lower Temperature

Spatial scanning of the synchronously generated second- and third-order intermodulation distortion in superconducting resonators uncovers local nonlinearity hot spots, and possible time reversal symmetry breaking, using a simple probe fashioned from coaxial cable. It is clear that even and odd order nonlinearity in these samples do not share the same physical origins, because their temperature and static magnetic field dependences are quite different. 2nd order intermodulation distortion remains strong in these measurements as the temperature continues to drop belowTCto 77 K even though the 3rd order peaks nearTCand becomes smaller at lower temperature as predicted by the nonlinear Meissner effect. Both YBa2Cu3O7and Tl2Ba2CaCu2O8resonators of the same structure exhibit similar temperature dependence in the second order with second order remaining high at lower temperature. The YBa2Cu3O7sample has lower third-order intermodulation distortion with a pronounced peak atTC.

scholarly journals Origin of the residual line width under frequency-switched Lee–Goldburg decoupling in MAS solid-state NMR

2020 ◽  
Vol 1 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Johannes Hellwagner ◽  
Liam Grunwald ◽  
Manuel Ochsner ◽  
Daniel Zindel ◽  
Beat H. Meier ◽  
...  
Keyword(s):  
Solid State ◽  
Line Width ◽  
Floquet Theory ◽  
Pulse Sequence ◽  
Second Order ◽  
Magic Angle ◽  
Line Broadening ◽  
Third Order ◽  
Order Error ◽  
Error Terms

Abstract. Homonuclear decoupling sequences in solid-state nuclear magnetic resonance (NMR) under magic-angle spinning (MAS) show experimentally significantly larger residual line width than expected from Floquet theory to second order. We present an in-depth theoretical and experimental analysis of the origin of the residual line width under decoupling based on frequency-switched Lee–Goldburg (FSLG) sequences. We analyze the effect of experimental pulse-shape errors (e.g., pulse transients and B1-field inhomogeneities) and use a Floquet-theory-based description of higher-order error terms that arise from the interference between the MAS rotation and the pulse sequence. It is shown that the magnitude of the third-order auto term of a single homo- or heteronuclear coupled spin pair is important and leads to significant line broadening under FSLG decoupling. Furthermore, we show the dependence of these third-order error terms on the angle of the effective field with the B0 field. An analysis of second-order cross terms is presented that shows that the influence of three-spin terms is small since they are averaged by the pulse sequence. The importance of the inhomogeneity of the radio-frequency (rf) field is discussed and shown to be the main source of residual line broadening while pulse transients do not seem to play an important role. Experimentally, the influence of the combination of these error terms is shown by using restricted samples and pulse-transient compensation. The results show that all terms are additive but the major contribution to the residual line width comes from the rf-field inhomogeneity for the standard implementation of FSLG sequences, which is significant even for samples with a restricted volume.

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