scholarly journals Efficient and reproducible somatic embryogenesis and micro propagation in tomato via novel structures -Rhizoid Tubers

2018 ◽  
Author(s):  
Wajeeha Saeed ◽  
Saadia Naseem ◽  
Daniyal Gohar ◽  
Zahid Ali
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Light Conditions ◽  
Indirect Organogenesis ◽  
Novel Structure ◽  
Micro Propagation ◽  
Novel Structures ◽  
Indole 3 Acetic Acid

AbstractAn improved and highly reproducible system for invitro regeneration via somatic embryogenesis (S.E), applicable to several varieties of tomato (cv. Riogrande, cv. Roma grande, hybrid 17905 and model cv. M82) has been developed. First, we developed a conventional indirect organogenesis for all four varieties used in this study. The cotyledons and hypocotyls of 6-day-old tomato were used as explants (1-2 cm) for callus induction (CI) on different callus induction media (CIM) T0 – T12 (6-Benzylaminopurine BAP, NAA Naphthalene acetic acid, ZEA Zeatin, IAA Indole-3-acetic acid, KIN Kinetin). Maximum CI response was seen on CIMT6 (0.5 mg/L NAA, 1 mg/L BAP) and CIMT7 (2 mg/L IAA, 2 mg/L NAA, 2 mg/L BAP, 4mg/L KIN) in a period of 2 weeks for commercial varieties cvs. Riogrande and Roma. However, cv. M82 responded after 4 weeks to a combination of treatments [CIMT6 (0.5 mg/L NAA + 1 mg/L BAP) and CIMT8 (2 mg/L IAA + 2 mg/L NAA + 2 mg/L BAP + 4 mg/L ZEA)] for the production of calli. The Riogrande, being the most responsive commercial variety, was selected for invitro morphogenesis via S.E. During S.E. young cotyledons and hypocotyls explants were tested on media with different ranges of pH (3 – 7) supplemented with 0.5 and 2 mg/L NAA. Resultantly, numerous rhizoids (~38) were produced from each explant at pH4 in dark conditions. Further incubation of each rhizoid under light conditions led to the formation of a novel structure - rhizoid tubers (RTBs) on MS media supplemented with 5 mg/L TDZ/BAP at pH4. We observed that only lower pH-induced rhizoids and RTBs regenerated into multiple individual shoots on media at normal pH (5.8). The RTBs led to a complete plantlets regeneration in 45 days compared to the conventional invitro morphogenesis (60 days).

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

Plant Regeneration from Cultured Medicago truncatula with Particular Reference to Abscisic Acid and Light Treatments

1998 ◽  
Vol 46 (1) ◽  
pp. 151 ◽  
Author(s):  
K. E. Nolan ◽  
R. J. Rose
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Plant Regeneration ◽  
Medicago Truncatula ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Marked Inhibition ◽  
Embryo Formation ◽  
Auxin Concentration

Medicago truncatula (Jemalong 2HA) can be regenerated by somatic embryogenesis utilising 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). There is a requirement for both NAA and BAP for callus induction and embryo formation. There is no requirement for a drop in auxin concentration to induce embryos. Abscisic acid (ABA) when present with NAA and BAP during embryo formation at a concentration of 1 µM, increases the number of embryos per callus. The ABA treatment stimulates embryo numbers in both light and darkness. The conversion efficiency of embryo to plant is unchanged irrespective of the presence of ABA during embryo formation, indicating that ABA does not improve the regeneration of the embryos once formed. Importantly, the presence of light in the embryo formation period causes a marked inhibition of embryo conversion.

scholarly journals CALLUS INDUCTION ON BANANA FLOWER’S EXPLANT IN VITRO USING 2,4-DICHLOROPHENOXYACETIC (2,4-D)

2018 ◽  
Vol 22 (2) ◽  
pp. 66
Author(s):  
RINDANG - DWIYANI ◽  
HESTIN - YUSWATI ◽  
UTAMI -
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Regeneration Medium ◽  
Indirect Organogenesis ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Banana Cultivar

ABSTRACT  The objective of the study was to obtain the best 2,4-D concentration on callus induction of the banana flowers in banana propagation using indirect organogenesis method. Kesuna, local banana cultivar obtained from Sembung Gede, Tabanan was used as explant material. Callus induction was performed using 2,4-Dichlorophenoxyacetic acid with concentration of 0; 0.5; 1.0; 1.5 and 2.0 ppm. Each treatment was represented by 3 bottles and each bottle was planted with 3 explants, so each treatment was represented by 9 explants of banana flowers. The results showed that the concentration of 2.0 ppm 2.4-D induced callus with the fastest time and gave the highest percentage of the explants producing callus. The calluses were subsequently subcultured into regeneration medium using 0.5 mg/L Benzylaminopurine (BAP) and 0.005 mg/L Napthaleneaceticacid (NAA). The calluses were subsequently sub-cultured into a regeneration medium using 0.5 ppm (BAP) and 0.005 ppm Naphthalene acetic acid (NAA) to induce shoots and roots and performed plantlets.   Keywords: 2,4-Dichlorophenoxyacetic acid, banana’s flowers, callus

Immuno-cytochemical localization of indole-3-acetic acid during induction of somatic embryogenesis in cultured sunflower embryos

Planta ◽  
2002 ◽  
Vol 215 (4) ◽  
pp. 577-583 ◽  
Author(s):  
Cl�ment Thomas ◽  
Roberte Bronner ◽  
Jean Molinier ◽  
Els Prinsen ◽  
Harry van Onckelen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Cytochemical Localization ◽  
Indole 3 Acetic Acid

scholarly journals Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.)

2014 ◽  
Vol 32 (2) ◽  
pp. 170-179 ◽  
Author(s):  
Hernando Criollo ◽  
Margarita Perea ◽  
Mariano Toribio ◽  
Johanna Muñoz
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Plant Propagation ◽  
Cotyledonary Leaf

Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 mM of NAA were added to the medium with sucrose (50.0 and 263.1 mM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 mM of NAA ; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 mM of NAA + 263.1 mM of sucrose (32%).

scholarly journals Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1634
Author(s):  
Mª Teresa Martínez ◽  
Isabel Arrillaga ◽  
Ester Sales ◽  
María Amparo Pérez-Oliver ◽  
Mª del Carmen González-Mas ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Phytophthora Cinnamomi ◽  
Expression Profiles ◽  
Holm Oak ◽  
Naphthalene Acetic Acid ◽  
Shoot Cultures ◽  
Mature Trees ◽  
Phenylpropanoid Biosynthesis ◽  
Indole 3 Acetic Acid

Holm oak populations have deteriorated drastically due to oak decline syndrome. The first objective of the present study was to investigate the use of axillary budding and somatic embryogenesis (SE) to propagate asymptomatic holm oak genotypes identified in disease hotspots in Spain. Axillary budding was achieved in two out of six tolerant genotypes from the south-western region and in two out of four genotypes from the Mediterranean region. Rooting of shoots cultured on medium supplemented with 3 mg L−1 of indole-3-acetic acid plus 0.1 mg L−1 α-naphthalene acetic acid was achieved, with rates ranging from 8 to 36%. Shoot cultures remained viable after cold storage for 9–12 months; this procedure is therefore suitable for medium-term conservation of holm oak germplasm. SE was induced in two out of the three genotypes tested, by using nodes and shoot tips cultured in medium without plant growth regulators. In vitro cloned progenies of the tolerant genotypes PL-T2 and VA5 inhibited growth of Phytophthora cinnamomi mycelia when exposed to the oomycete in vitro. Significant differences in total phenol contents and in the expression profiles of genes regulating phenylpropanoid biosynthesis were observed between in vitro cultured shoots derived from tolerant trees and cultures established from control genotypes.

scholarly journals Clonal micropropagation of decorative cereal Molinia caerulea (L.) Moench

2020 ◽  
Vol 21 (6) ◽  
pp. 713-720
Author(s):  
T. G. Lekontseva ◽  
A. V. Fedorov
Keyword(s):  
Acetic Acid ◽  
Survival Rate ◽  
Shoot Length ◽  
Ms Medium ◽  
Molinia Caerulea ◽  
Clonal Micropropagation ◽  
Micro Propagation ◽  
Shoot Size ◽  
Free Media ◽  
Indole 3 Acetic Acid

The research is devoted to the development of clonal micro-propagation technology of the decorative cereal Molinia caerulea (L.) Moench. The concentration of 2.0 mg /l of cytokinin 6-benzylaminopurine (6-BAP) contributed to obtaining the maximum number of microshoots: 6.3 and 7.9 pcs. on Anderson's and Murashige-Skoog's (MS) media, respectively, which exceeded the control (by 4.7 and 6.3, respectively, with LSD05 = 2.3). The length shoots were observed on hormone-free media, this indicator significantly decreased with an increase in 6-BAP content. On Anderson and MS media with 1.0 mg/l 6-BAP, the shoot length averaged 21.5 and 26.4 mm, respectively, which made it possible to transplant them for rooting, bypassing planting on a medium for elongation. The inclusion of the Siliplant micro-fertilizer in the MS medium at doses of 1.0 and 2.0 ml/l contributed to a significant increase in shoot size, by 16.7 and 10.7 mm (LSD05 = 8.9), respectively, in comparison with the control (MS). It is recommended to use Anderson's medium and 0.5 mg/l of indole-3-acetic acid as a medium for rhizogenesis: after two weeks of cultivation, the regenerants had a standard appearance with developed roots suitable for planting for adaptation. At the adaptation stage, watering the substrate with the biofungicide «Trichoderma veride» according to the instructions and a cereals single spraying with the micro-fertilizer «Siliplant» at a dose of 1.5 ml/l contributed to their 100 % survival rate.

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