scholarly journals Neuropeptide B mediates female sexual receptivity in medaka fish, acting in a female-specific but reversible manner

2019 ◽  
Author(s):  
Towako Hiraki-Kajiyama ◽  
Junpei Yamashita ◽  
Keiko Yokoyama ◽  
Kohei Hosono ◽  
Yukika Kawabata-Sakata ◽  
...  
Keyword(s):  
Mating Behavior ◽  
Transcriptional Activation ◽  
Sex Steroid ◽  
Critical Element ◽  
Specific Expression ◽  
Behavioral Studies ◽  
Estrogen Responsive Element ◽  
Mating Behaviors ◽  
Responsive Element ◽  
Female Specific

AbstractMale and female animals display innate sex-specific mating behaviors. Among vertebrates, teleosts are unique in that altering the adult sex steroid milieu can reverse sex-typical mating behaviors, suggesting sexual lability of their brains. In the teleost medaka, neuropeptide B (NPB) is expressed female-specifically in the brain nuclei implicated in mating behavior. Here, we demonstrate that NPB is a direct mediator of estrogen action on female mating behavior, acting in a female-specific but reversible manner. Analysis of regulatory mechanisms revealed that the female-specific expression of NPB is dependent on direct transcriptional activation by estrogen via an estrogen-responsive element and is reversed in response to changes in the adult sex steroid milieu. Behavioral studies of NPB knockouts revealed that female-specific NBP mediates female receptivity to male courtship. The female-specific NPB signaling identified herein is presumably a critical element of the neural circuitry underlying sexual dimorphism and lability of mating behaviors in teleosts.

scholarly journals Neuropeptide B mediates female sexual receptivity in medaka fish, acting in a female-specific but reversible manner

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Towako Hiraki-Kajiyama ◽  
Junpei Yamashita ◽  
Keiko Yokoyama ◽  
Yukiko Kikuchi ◽  
Mikoto Nakajo ◽  
...  
Keyword(s):  
Mating Behavior ◽  
Transcriptional Activation ◽  
Sex Steroid ◽  
Critical Element ◽  
Specific Expression ◽  
Behavioral Studies ◽  
Estrogen Responsive Element ◽  
Mating Behaviors ◽  
Responsive Element ◽  
Female Specific

Male and female animals display innate sex-specific mating behaviors. In teleost fish, altering the adult sex steroid milieu can effectively reverse sex-typical mating behaviors, suggesting remarkable sexual lability of their brains as adults. In the teleost medaka, neuropeptide B (NPB) is expressed female-specifically in the brain nuclei implicated in mating behavior. Here, we demonstrate that NPB is a direct mediator of estrogen action on female mating behavior, acting in a female-specific but reversible manner. Analysis of regulatory mechanisms revealed that the female-specific expression of NPB is dependent on direct transcriptional activation by estrogen via an estrogen-responsive element and is reversed in response to changes in the adult sex steroid milieu. Behavioral studies of NPB knockouts revealed that female-specific NBP mediates female receptivity to male courtship. The female-specific NPB signaling identified herein is presumably a critical element of the neural circuitry underlying sexual dimorphism and lability of mating behaviors in teleosts.

scholarly journals Estrogen Induces Selective Transcription of Caveolin1 Variants in Human Breast Cancer through Estrogen Responsive Element-Dependent Mechanisms

2020 ◽  
Vol 21 (17) ◽  
pp. 5989
Author(s):  
Antonella Romano ◽  
Antonia Feola ◽  
Antonio Porcellini ◽  
Vincenzo Gigantino ◽  
Maurizio Di Bonito ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcriptional Activation ◽  
Protein Isoforms ◽  
Regulatory Control ◽  
Physiological Processes ◽  
Regulatory Circuit ◽  
Estrogen Responsive Element ◽  
Histone H3 Methylation ◽  
Responsive Element ◽  
H3 Acetylation

The estrogen receptor (ER) signaling regulates numerous physiological processes mainly through activation of gene transcription (genomic pathways). Caveolin1 (CAV1) is a membrane-resident protein that behaves as platform to enable different signaling molecules and receptors for membrane-initiated pathways. CAV1 directly interacts with ERs and allows their localization on membrane with consequent activation of ER-non-genomic pathways. Loss of CAV1 function is a common feature of different types of cancers, including breast cancer. Two protein isoforms, CAV1α and CAV1β, derived from two alternative translation initiation sites, are commonly described for this gene. However, the exact transcriptional regulation underlying CAV1 expression pattern is poorly elucidated. In this study, we dissect the molecular mechanism involved in selective expression of CAV1β isoform, induced by estrogens and downregulated in breast cancer. Luciferase assays and Chromatin immunoprecipitation demonstrate that transcriptional activation is triggered by estrogen-responsive elements embedded in CAV1 intragenic regions and DNA-binding of estrogen-ER complexes. This regulatory control is dynamically established by local chromatin changes, as proved by the occurrence of histone H3 methylation/demethylation events and association of modifier proteins as well as modification of H3 acetylation status. Thus, we demonstrate for the first time, an estrogen-ERs-dependent regulatory circuit sustaining selective CAV1β expression.

scholarly journals Sequence Elements Necessary for Transcriptional Activation of BAD1 in the Yeast Phase of Blastomyces dermatitidis

2004 ◽  
Vol 3 (3) ◽  
pp. 785-794 ◽  
Author(s):  
Peggy J. Rooney ◽  
Bruce S. Klein
Keyword(s):  
Transcriptional Activation ◽  
Histoplasma Capsulatum ◽  
Upstream Region ◽  
Blastomyces Dermatitidis ◽  
Specific Gene ◽  
Specific Expression ◽  
Sequence Elements ◽  
Putative Transcription Factor ◽  
Yeast Phase ◽  
Responsive Element

ABSTRACT Blastomyces dermatitidis is a dimorphic fungal pathogen that converts from mycelia or conidia to a host-adapted yeast morphotype upon infection. Conversion to the yeast form is accompanied by the production of the virulence factor BAD1. Yeast-phase-specific expression of BAD1 is transcriptionally regulated, and its promoter shares homology with that of the yeast-phase-specific gene YPS3 of Histoplasma capsulatum. Serial truncations of the BAD1 upstream region were fused to the lacZ reporter to define functional areas in the promoter. Examination of P BAD1 -lacZ fusions in B. dermatitidis indicated that BAD1 transcription is upregulated in the yeast phase. The 63-nucleotide box A region conserved in the YPS3 upstream region was shown to be an essential component of the minimal BAD1 promoter. A matched P YPS3 -lacZ construct indicated that this same region was needed for minimal YPS3 promoter activity in B. dermatitidis transformants. Reporter activity in H. capsulatum transformants similarly showed a requirement for box A in the minimal BAD1 promoter. Several putative transcription factor binding sites were identified within box A of BAD1. Replacement of two of these predicted sites within box A—a cAMP responsive element and a Myb binding site—sharply reduced transcriptional activity, indicating that these regions are critical in dictating the yeast-phase-specific expression of this crucial virulence determinant of B. dermatitidis.

scholarly journals A unique mating pattern of Panorpodes kuandianensis (Mecoptera: Panorpodidae)

2017 ◽  
Vol 86 (3) ◽  
pp. 229-237 ◽  
Author(s):  
Xin Tong ◽  
Lu Jiang ◽  
Bao-Zhen Hua
Keyword(s):  
Mating Behavior ◽  
Sexual Conflict ◽  
Sperm Transfer ◽  
Nuptial Gift ◽  
Mating Pattern ◽  
End To End ◽  
Mating Behaviors ◽  
First Time

Sexually reproductive insects exhibit diverse mating behaviors. However, the mating pattern remains unknown for Panorpodes of Panorpodidae to date. In this study, we investigated the mating behavior and copulatory mechanism of the short-faced scorpionfly Panorpodes kuandianensis Zhong, Zhang and Hua, 2011 for the first time. The results show that the male provides a salivary mass as a nuptial gift to the female and starts to copulate with the female in a V-shaped position, then changes to an end-to-end position by temporarily twisting the female abdominal segments VII−IX by 180°. During mating the basal processes and the basal teeth of the gonostyli and the hypandrium are used to obtain copulation and sustain the coupling of genitalia to secure successful sperm transfer. This unique mating pattern is greatly different from that of other Mecoptera reported and is likely evolved as an adaptation in the context of sexual conflict.

scholarly journals Real-Time Challenging of ERα Y537S Mutant Transcriptional Activity in Living Cells

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 54-64
Author(s):  
Manuela Cipolletti ◽  
Sara Pescatori ◽  
Filippo Acconcia
Keyword(s):  
Cell Line ◽  
Transcriptional Activity ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Patient Treatment ◽  
Estrogen Responsive Element ◽  
Specific Proteins ◽  
Responsive Element ◽  
Mcf 7

Metastatic estrogen receptor α (ERα)-expressing breast cancer (BC) occurs after prolonged patient treatment with endocrine therapy (ET) (e.g., aromatase inhibitors—AI; 4OH-tamoxifen—4OH-Tam). Often these metastatic BCs express a mutated ERα variant (e.g., Y537S), which is transcriptionally hyperactive, sustains uncontrolled proliferation, and renders tumor cells insensitive to ET drugs. Therefore, new molecules blocking hyperactive Y537S ERα mutation transcriptional activity are requested. Here we generated an MCF-7 cell line expressing the Y537S ERα mutation stably expressing an estrogen-responsive element (ERE) promoter, which activity can be monitored in living cells. Characterization of this cell line shows both hyperactive basal transcriptional activity with respect to normal MCF-7 cells, which stably express the same ERE-based promoter and a decreased effect of selective ER downregulators (SERDs) in reducing Y537S ERα mutant transcriptional activity with respect to wild type ERα transcriptional activity. Kinetic profiles of Y537S ERα mutant-based transcription produced by both drugs inducing receptor degradation and siRNA-mediated depletion of specific proteins (e.g., FOXA1 and caveolin1) reveals biphasic dynamics of the inhibition of the receptor-regulated transcriptional effects. Overall, we report a new model where to study the behavior of the Y537S ERα mutant that can be used for the identification of new targets and pathways regulating the Y537S ERα transcriptional activity.

scholarly journals Chromosome Y pericentric heterochromatin is a primary target of HSF1 in male cells

Chromosoma ◽  
2021 ◽  
Vol 130 (1) ◽  
pp. 53-60
Author(s):  
Jessica Penin ◽  
Solenne Dufour ◽  
Virginie Faure ◽  
Sabrina Fritah ◽  
Daphné Seigneurin-Berny ◽  
...  
Keyword(s):  
Heat Shock ◽  
Transcriptional Activation ◽  
Human Fibroblasts ◽  
Pericentric Heterochromatin ◽  
Chromosome 9 ◽  
Nuclear Accumulation ◽  
Critical Element ◽  
Heat Shock Factor 1 ◽  
Primary Target ◽  
Chromosome Y

AbstractThe heat shock factor 1 (HSF1)-dependent transcriptional activation of human pericentric heterochromatin in heat-shocked cells is the most striking example of transcriptional activation of heterochromatin. Until now, pericentric heterochromatin of chromosome 9 has been identified as the primary target of HSF1, in both normal and tumor heat-shocked cells. Transcriptional awakening of this large genomic region results in the nuclear accumulation of satellite III (SATIII) noncoding RNAs (ncRNAs) and the formation in cis of specific structures known as nuclear stress bodies (nSBs). Here, we show that, in four different male cell lines, including primary human fibroblasts and amniocytes, pericentric heterochromatin of chromosome Y can also serve as a unique primary site of HSF1-dependent heterochromatin transcriptional activation, production of SATIII ncRNA, and nucleation of nuclear stress bodies (nSBs) upon heat shock. Our observation suggests that the chromosomal origin of SATIII transcripts in cells submitted to heat shock is not a determinant factor as such, but that transcription of SATIII repetitive units or the SATIII ncRNA molecules is the critical element of HSF1-dependent transcription activation of constitutive heterochromatin.

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