scholarly journals Differential gene expression and functional pathway enrichment in colon cell line CCD 841 CoN (CRL-1790) transfected with miR-mimics miR-18b, miR-142-3p, miR-155, and miR-890

2019 ◽  
Author(s):  
JM Robinson
Keyword(s):  
Cell Line ◽  
Bioinformatic Analysis ◽  
Gene Set Enrichment ◽  
Over Expression ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Differential Gene ◽  
Advanced Analysis ◽  
Functional Pathway ◽  
Reported Data

AbstractThis report is a bioinformatic analysis of samples from NCBI GEO database series GS132501. It includes previously un-reported data for miR-mimic over-expression of the miRNAs miR-18b, miR-155, miR-142-3p, and miR-890 in the ATCC CRL-1790 cell line. Data analysis was performed using Nanostring nSolver 4.0 and Advanced Analysis Module 2.0 plugin (Nanostring MAN-10030-03). Bioinformatic methods utilized include pathway scoring, differential expression (DE), and gene-set enrichment (GSE) analyses. Findings, with full supplementary data, provide a community resource for effects of dysregulation of these miRNAs in a colon ‘epithelial-like’ cell line.

Formation of hydrogen peroxide in cell culture media by apple polyphenols and its effect on antioxidant biomarkers in the colon cell line HT-29

2009 ◽  
Vol 53 (10) ◽  
pp. 1226-1236 ◽  
Author(s):  
Phillip Bellion ◽  
Melanie Olk ◽  
Frank Will ◽  
Helmut Dietrich ◽  
Matthias Baum ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Cell Line ◽  
Culture Media ◽  
Cell Culture Media ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Ht 29

scholarly journals Determination of cell death induced by lovastatin on human colon cell line HT29 using comet assay

2011 ◽  
Vol 205 ◽  
pp. S116-S117
Author(s):  
M. Rezaei ◽  
H. Kalantari ◽  
M. HashemiTabar ◽  
M. Jafari ◽  
Z. Bahadori
Keyword(s):  
Cell Death ◽  
Comet Assay ◽  
Cell Line ◽  
Human Colon ◽  
Human Colon Cell Line ◽  
Colon Cell ◽  
Colon Cell Line

scholarly journals Novel Synthesis Method of Micronized Ti-Zeolite Na-A and Cytotoxic Activity of Its Silver Exchanged Form

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
H. F. Youssef ◽  
W. H. Hegazy ◽  
H. H. Abo-almaged ◽  
G. T. El-Bassyouni
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Synthesis Method ◽  
Carcinoma Cell Line ◽  
Breast Adenocarcinoma ◽  
Synthetic Process ◽  
Colon Cell ◽  
Colon Cell Line

The core-shell method is used as a novel synthetic process of micronized Ti-Zeolite Na-A which involves calcination at 700°C of coated Egyptian Kaolin with titanium tetrachloride in acidic medium as the first step. The produced Ti-coated metakaolinite is subjected to microwave irradiation at low temperature of 80°C for 2 h. The prepared micronized Ti-containing Zeolites-A (Ti-Z-A) is characterized by FTIR, XRF, XRD, SEM, and EDS elemental analysis. Ag-exchanged form of Ti-Z-Ag is also prepared and characterized. The Wt% of silver exchanged onto the Ti-Zeolite structure was determined by atomic absorption spectra. Thein vitrocytotoxic activity of Ti-Z-Ag against human hepatocellular carcinoma cell line (HePG2), colon cell line carcinoma (HCT116), lung carcinoma cell line (A549), and human Caucasian breast adenocarcinoma (MCF7) is reported. The results were promising and revealed that the exchanged Ag form of micronized Ti-Zeolite-A can be used as novel antitumor drug.

scholarly journals Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

Medicina ◽  
2019 ◽  
Vol 55 (7) ◽  
pp. 349 ◽  
Author(s):  
Mayra A. Mendez-Encinas ◽  
Elizabeth Carvajal-Millan ◽  
Agustín Rascón-Chu ◽  
Humberto Astiazarán-García ◽  
Dora E. Valencia-Rivera ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Cell Line ◽  
Human Colon ◽  
Colon Cells ◽  
Antioxidant Power ◽  
Human Colon Cell Line ◽  
Colon Cell ◽  
Colon Cell Line

Background and objectives: Arabinoxylans (AX) can gel and exhibit antioxidant capacity. Previous studies have demonstrated the potential application of AX microspheres as colon-targeted drug carriers. However, the cytotoxicity of AX gels has not been investigated so far. Therefore, the aim of the present study was to prepare AX-based particles (AXM) by coaxial electrospraying method and to investigate their antioxidant potential and cytotoxicity on human colon cells. Materials and Methods: The gelation of AX was studied by monitoring the storage (G′) and loss (G′′) moduli. The morphology of AXM was evaluated using optical and scanning electron microscopy (SEM). The in vitro antioxidant activity of AX before and after gelation was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. In addition, the effect of AX and AXM on the proliferation of human colon cells (CCD 841 CoN) was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The final G′ and G′′ values for AX gels were 293 and 0.31 Pa, respectively. AXM presented spherical shape and rough surface with a three-dimensional and porous network. The swelling ratio and mesh size of AXM were 35 g water/g AX and 27 nm, respectively. Gelation decreased the antioxidant activity of AX by 61–64 %. AX and AXM did not affect proliferation or show any toxic effect on the normal human colon cell line CCD 841 CoN. Conclusion: The results indicate that AXM could be promising biocompatible materials with antioxidant activity.

scholarly journals ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF MORINGA OLEIFERA

2016 ◽  
Vol 8 (4) ◽  
pp. 54 ◽  
Author(s):  
Burli Sanganna ◽  
Havagiray R. Chitme ◽  
Khanvilkar Vrunda ◽  
Mohsin J. Jamadar
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Cell Line ◽  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Phytochemical Constituents ◽  
Ht 29

Objective: The aim of the study was to investigate the ethanolic and aqueous extract of leaves of Moringa oleifera for phytochemical constituents, antiproliferative and antioxidant activity.Methods: The ethanolic extract of leaves of Moringa oleifera, belonging to the family Moringaceae was prepared by using soxhlet apparatus and aqueous extract was prepared by using maceration process. The extract was evaluated for its phytochemical constituents. The antiproliferative effects of both extracts were checked by using MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) assay on HT-29 colon cell line and the antioxidant activity were checked by using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. In antiproliferative and antioxidant activity the 5-FU (5-fluro uracil) and Ascorbic acid used as a standard drug for present results conclusion respectively.Results: The results obtained in MTT assay shown that ethanolic extract of Moringa oleifera had a more potent antiproliferative effect (growth inhibition of 62.25% at 100 μg/ml) on HT-29 colon cell line as compared to aqueous extract (% growth inhibition of 27.86 at 100 μg/ml) of Moringa oleifera. The ethanolic extract of Moringa oleifera shown more potent antioxidant activity (% inhibition of ethanolic 75.57 at 100 μg/ml) than aqueous extract (38.16 at 100 μg/ml) of Moringa oleifera. The activity shown by the extract is concentration dependent.Conclusion: In the present study we have investigated that the effect of ethanolic and aqueous leaves extracts of Moringa oleifera possess antiproliferative and antioxidant properties.

Glycine-extended gastrin acts as an autocrine growth factor in a nontransformed colon cell line

1997 ◽  
Vol 113 (5) ◽  
pp. 1576-1588 ◽  
Author(s):  
F Hollande ◽  
A Imdahl ◽  
T Mantamadiotis ◽  
GD Ciccotosto ◽  
A Shulkes ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Colon Cell ◽  
Colon Cell Line

Control of Cell pH in the T84 Colon Cell Line

2000 ◽  
Vol 177 (2) ◽  
pp. 149-157 ◽  
Author(s):  
M.A. Ramirez ◽  
R. Toriano ◽  
M. Parisi ◽  
G. Malnic
Keyword(s):  
Cell Line ◽  
Cell Ph ◽  
Colon Cell ◽  
Colon Cell Line
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