Formation of hydrogen peroxide in cell culture media by apple polyphenols and its effect on antioxidant biomarkers in the colon cell line HT-29

2009 ◽  
Vol 53 (10) ◽  
pp. 1226-1236 ◽  
Author(s):  
Phillip Bellion ◽  
Melanie Olk ◽  
Frank Will ◽  
Helmut Dietrich ◽  
Matthias Baum ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Cell Line ◽  
Culture Media ◽  
Cell Culture Media ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Ht 29

scholarly journals ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF MORINGA OLEIFERA

2016 ◽  
Vol 8 (4) ◽  
pp. 54 ◽  
Author(s):  
Burli Sanganna ◽  
Havagiray R. Chitme ◽  
Khanvilkar Vrunda ◽  
Mohsin J. Jamadar
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Cell Line ◽  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Phytochemical Constituents ◽  
Ht 29

Objective: The aim of the study was to investigate the ethanolic and aqueous extract of leaves of Moringa oleifera for phytochemical constituents, antiproliferative and antioxidant activity.Methods: The ethanolic extract of leaves of Moringa oleifera, belonging to the family Moringaceae was prepared by using soxhlet apparatus and aqueous extract was prepared by using maceration process. The extract was evaluated for its phytochemical constituents. The antiproliferative effects of both extracts were checked by using MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) assay on HT-29 colon cell line and the antioxidant activity were checked by using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. In antiproliferative and antioxidant activity the 5-FU (5-fluro uracil) and Ascorbic acid used as a standard drug for present results conclusion respectively.Results: The results obtained in MTT assay shown that ethanolic extract of Moringa oleifera had a more potent antiproliferative effect (growth inhibition of 62.25% at 100 μg/ml) on HT-29 colon cell line as compared to aqueous extract (% growth inhibition of 27.86 at 100 μg/ml) of Moringa oleifera. The ethanolic extract of Moringa oleifera shown more potent antioxidant activity (% inhibition of ethanolic 75.57 at 100 μg/ml) than aqueous extract (38.16 at 100 μg/ml) of Moringa oleifera. The activity shown by the extract is concentration dependent.Conclusion: In the present study we have investigated that the effect of ethanolic and aqueous leaves extracts of Moringa oleifera possess antiproliferative and antioxidant properties.

scholarly journals The effect of hydrogen peroxide produced during ultraviolet disinfection of CHO cell culture media

2017 ◽  
Vol 61 ◽  
pp. 147-155 ◽  
Author(s):  
Emma V. Dare ◽  
Michelle Gabriel ◽  
Afroza Begum ◽  
Michael Sasges ◽  
Marc G. Aucoin
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Culture Media ◽  
Ultraviolet Disinfection ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

In vitro cytocompatibility testing of oxidative degradation products

2021 ◽  
pp. 088391152110031
Author(s):  
Scott M Herting ◽  
Mary Beth B Monroe ◽  
Andrew C Weems ◽  
Sam T Briggs ◽  
Grace K Fletcher ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Culture Media ◽  
Degradation Products ◽  
Oxidative Degradation ◽  
Cell Culture Media ◽  
Novel Approach ◽  
Gene And Protein Expression

Implantable medical devices must undergo thorough evaluation to ensure safety and efficacy before use in humans. If a device is designed to degrade, it is critical to understand the rate of degradation and the degradation products that will be released. Oxidative degradation is typically modeled in vitro by immersing materials or devices in hydrogen peroxide, which can limit further analysis of degradation products in many cases. Here we demonstrate a novel approach for testing the cytocompatibility of degradation products for oxidatively-degradable biomaterials where the materials are exposed to hydrogen peroxide, and then catalase enzyme is used to convert the hydrogen peroxide to water and oxygen so that the resulting aqueous solution can be added to cell culture media. To validate our results, expected degradation products are also synthesized then added to cell culture media. We used these methods to evaluate the cytocompatibility of degradation products from an oxidatively-degradable shape memory polyurethane designed in our lab and found that the degradation of these polymers is unlikely to cause a cytotoxic response in vivo based on the guidance provided by ISO 10993-5. These methods may also be applicable to other biocompatibility tests such as tests for mutagenicity or systemic toxicity, and evaluations of cell proliferation, migration, or gene and protein expression.

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