scholarly journals Initiation of stem cell differentiation involves cell cycle-dependent regulation of developmental genes by Cyclin D

2016 ◽  
Vol 30 (4) ◽  
pp. 421-433 ◽  
Author(s):  
Siim Pauklin ◽  
Pedro Madrigal ◽  
Alessandro Bertero ◽  
Ludovic Vallier
Keyword(s):  
Cell Cycle ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Cyclin D ◽  
Developmental Genes ◽  
Cycle Dependent

scholarly journals Unraveling the Control of Cell Cycle Periods during Intestinal Stem Cell Differentiation

2018 ◽  
Vol 115 (11) ◽  
pp. 2250-2258 ◽  
Author(s):  
Richard Ballweg ◽  
Suengwon Lee ◽  
Xiaonan Han ◽  
Philip K. Maini ◽  
Helen Byrne ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Intestinal Stem Cell

scholarly journals Regulating cell fate of human amnion epithelial cells using natural compounds: an example of enhanced neural and pigment differentiation by 3,4,5-tri-O-caffeoylquinic acid

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Meriem Bejaoui ◽  
Farhana Ferdousi ◽  
Yun-Wen Zheng ◽  
Tatsuya Oda ◽  
Hiroko Isoda
Keyword(s):  
Cell Cycle ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Natural Compounds ◽  
Polyphenolic Compound ◽  
Human Amnion ◽  
Caffeoylquinic Acid ◽  
Cycle Arrest ◽  
Amnion Epithelial Cells

AbstractOver the past years, Human Amnion Epithelial Cells (hAECs), a placental stem cell, are gaining higher attention from the scientific community as they showed several advantages over other types of stem cells, including availability, easy accessibility, reduced rejection rate, non-tumorigenicity, and minimal legal constraint. Recently, natural compounds are used to stimulate stem cell differentiation and proliferation and to enhance their disease-treating potential. A polyphenolic compound 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA) has been previously reported to induce human neural stem cell differentiation and may affect melanocyte stem cell differentiation as well. In this study, TCQA was tested on 3D cultured hAECs after seven days of treatment, and then, microarray gene expression profiling was conducted of TCQA-treated and untreated control cells on day 0 and day 7. Analyses revealed that TCQA treatment significantly enriched pigment and neural cells sets; besides, genes linked with neurogenesis, oxidation–reduction process, epidermal development, and metabolism were positively regulated. Interestingly, TCQA stimulated cell cycle arrest-related pathways and differentiation signaling. On the other hand, TCQA decreased interleukins and cytokines expression and this due to its anti-inflammatory properties as a polyphenolic compound. Results were validated to highlight the main activities of TCQA on hAECs, including differentiation, cell cycle arrest, and anti-inflammatory. This study highlights the important role of hAECs in regenerative medicine and the use of natural compounds to regulate their fate.

scholarly journals Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila

2018 ◽  
Vol 434 (1) ◽  
pp. 84-95 ◽  
Author(s):  
Pooja Flora ◽  
Sean Schowalter ◽  
SiuWah Wong-Deyrup ◽  
Matthew DeGennaro ◽  
Mohamad Ali Nasrallah ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Transcriptional Silencing ◽  
Stem Cell Differentiation ◽  
Germline Stem Cell

Cell Cycle Influence of Stem Cell Differentiation in Culture.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4194-4194
Author(s):  
Toska J. Zomorodian ◽  
Mehrdad Abedi ◽  
Gerri Dooner ◽  
Debbie Greer ◽  
Kevin Johnson ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stromal Cell ◽  
Cultured Cells ◽  
Cell Layer ◽  
Stem Cell Differentiation ◽  
S Phase ◽  
Time Point

Abstract Hierarchical models of hematopoiesis suppose an ordered system in which stem cells and progenitors with specific fixed differentiation potentials exist. We show that the potential of marrow stem cells to differentiate changes reversibly with cytokine-induced cell cycle transit. To address whether the cell cycle plays a role in the differentiation of stem cells, we co-cultured murine bone marrow Lin- Sca-1+ cells, at different points in their cycle, with the OP9-DL1 system. OP9-DL1 stromal cell layer has been transduced to allow T-cell differentiation in culture. We first induced cell cycle synchrony by exposing the isolated cells to a cytokine cocktail of TPO, Flt-3 and Stem Cell Factor. The cells were exposed to this primary culture for 0, 6, 24, 32 and 40 hours and were subsequently cultured on an OP9-DL1 stromal cell layer grown in 6-well plates. Cells were co-cultured for 8 days and 21 days, in the presence of IL-7 and Flt-3. Cultured cells were evaluated for CD4, CD8, B220, CD19, NK1.1, and Mac-1 surface markers, using flow cytometry. On Day 8, we found a significant hotspot at 32-hours (early-S phase) for B220+ cells (34.3 %), while Mac-1 positive cells demonstrated a 24-hour hotspot (18.1 %). As expected, terminal T and B-cell differentiation (CD 4, CD8, and CD19) was undetectable at 8 days. Three separate short-term (8 day) experiments have confirmed these data. Cells in culture for 21 days similarly show variation in differentiation outcome. CD4 cells demonstrate a peak at the 40 hour time point (mid-S phase) (69.9%), while CD8 positive cells were significantly increased at the 32 hour time point (34.4%). These data indicate both B and T cells show reversible differentiation fluxes linked to cell cycle. This work supports previous evidence that marrow hematopoiesis at the stem cell level is regulated on a continuum and that stem cells have reversible, cycle-related differentiation capacity.

Enhanced regulation of cell cycle and suppression of osteoblast differentiation molecular signatures by prostate cancer stem-like holoclones

2015 ◽  
Vol 68 (9) ◽  
pp. 692-702 ◽  
Author(s):  
Michael F Gallagher ◽  
Yvonne Salley ◽  
Cathy D Spillane ◽  
Brendan Ffrench ◽  
Salah El Baruni ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Osteoblast Differentiation ◽  
Epithelial Mesenchymal Transition ◽  
Stem Cell Differentiation ◽  
Molecular Signatures ◽  
Tumor Initiating Cells ◽  
Mesenchymal Transition

AimsTargeting the stem cell properties of tumor-initiating cells is an avenue through which cancer treatment may be improved. Before this can be achieved, so-called ‘cancer stem cell’ (CSC) models must be developed and characterized in specific malignancies.MethodsIn this study, holoclone formation assays were used to characterise stem-like molecular signatures in prostate cancer (PCa) cells.ResultsLNCaP and PC3 parent cells were capable of responding to stem cell differentiation morphogen retinoic acid (RA), suggesting the presence of inherent stem-like properties. LNCaP cells, which represent early, androgen-responsive disease, formed holoclones after twenty six days. PC3 cells, which represent advanced, metastatic, castration-resistant disease, formed holoclones after only six days. Holoclones displayed decreased expression of RA-genes, suggesting a more immature, less differentiated phenotype. Gene and microRNA arrays demonstrated that holoclones downregulated a number of stem cell differentiation regulators while displaying enhanced regulation of G2 to M transition and the mitotic spindle checkpoint components of the cell cycle. PC3 holoclones displayed pronounced downregulation of known regulators of osteoblast differentiation from mesenchymal stem cells and Epithelial Mesenchymal Transition.ConclusionsOur results suggest that some PCa cells retain the ability to transition to a more immature state in which differentiation and metastatic mechanisms are suppressed. The highlighting of osteoblast differentiation regulators in this mechanism is particularly notable, considering the propensity of PCa to metastasise to bone.

Sign in / Sign up

Export Citation Format

Share Document