scholarly journals MtNODULE ROOT1 and MtNODULE ROOT2 Are Essential for Indeterminate Nodule Identity

2018 ◽  
Vol 178 (1) ◽  
pp. 295-316 ◽  
Author(s):  
Kevin Magne ◽  
Jean-Malo Couzigou ◽  
Katharina Schiessl ◽  
Shengbin Liu ◽  
Jeoffrey George ◽  
...  
Keyword(s):  
Indeterminate Nodule

Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume

2011 ◽  
Vol 194 (2) ◽  
pp. 87-102 ◽  
Author(s):  
Isabel Margaret-Oliver ◽  
Wang Lei ◽  
Maribel Parada ◽  
Miguel A. Rodríguez-Carvajal ◽  
Juan C. Crespo-Rivas ◽  
...  
Keyword(s):  
Glycyrrhiza Uralensis ◽  
Sinorhizobium Fredii ◽  
Indeterminate Nodule

scholarly journals 1033 Endobronchial Sclerotic Neurofibroma: A Case Report of a Very Rare Benign Tumour

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
S Iftikhar ◽  
S Youssef ◽  
D Gey Van Pittius ◽  
S Ghosh ◽  
M Haris
Keyword(s):  
Case Report ◽  
Polypoid Lesion ◽  
Posterior Mediastinum ◽  
Rigid Bronchoscopy ◽  
Fibreoptic Bronchoscopy ◽  
Smoking History ◽  
General Anaesthetic ◽  
Indeterminate Nodule ◽  
Normocytic Anaemia ◽  
Rare Benign Tumour

Abstract Introduction Endobronchial neurofibromas are exceedingly rare benign lesions most commonly originating at the trachea. Primary pulmonary tumours of neurogenic origin such as peripheral nerve sheath tumours (PNSTs) are extremely rare, accounting for less than 0.2% of all lung tumours. Intrathoracic PNSTs are usually benign and are commonly found in the posterior mediastinum as schwannomas with female preponderance. We present the first reported occurrence of the sclerotic variant of endobronchial neurofibroma and the approach used in its definitive investigation and management. Case Report A 69-year-old Caucasian male with a 66 pack-year smoking history and a background of alcohol induced liver cirrhosis, peripheral vascular disease and dilated cardiomyopathy presented with dyspnoea and fatigue with severe normocytic anaemia. Computed Tomography (CT) chest, abdomen and pelvis revealed an indeterminate nodule at the secondary carina projecting into the bronchus intermedius (BI). Fibreoptic bronchoscopy showed a polypoid lesion with a vascular appearance. Under general anaesthetic, combined fibreoptic and rigid bronchoscopy was used to remove the lesion with rigid forceps. Gross histological appearance showed an 8x6x5mm pedunculated lesion with a 2mm diameter short stalk and histology of a sclerotic neurofibroma with fibroblasts and collagen, positively staining for S100 and LP10. This patient is due for follow up in 6-month with a CT chest to ensure no tumour recurrence. Conclusions Endobronchial neurofibromas appear highly vascular and demand extra caution at bronchoscopic intervention. Prompt, experienced thoracic surgical and intervention pulmonology input should always be sought. Combined approach of fibreoptic and rigid bronchoscopy allows better control of potential bleeding and the airway.

scholarly journals Genetic testing for indeterminate thyroid cytology: review and meta-analysis

2018 ◽  
Vol 25 (3) ◽  
pp. R163-R177 ◽  
Author(s):  
Sergio Vargas-Salas ◽  
José R Martínez ◽  
Soledad Urra ◽  
José Miguel Domínguez ◽  
Natalia Mena ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Meta Analysis ◽  
Molecular Testing ◽  
Clinical Use ◽  
Thyroid Cytology ◽  
Molecular Tests ◽  
Indeterminate Nodule ◽  
Health Need ◽  
The Impact

Thyroid cancer is the most frequent endocrine malignancy, and its incidence is increasing. A current limitation of cytological evaluation of thyroid nodules is that 20–25% are reported as indeterminate. Therefore, an important challenge for clinicians is to determine whether an indeterminate nodule is malignant, and should undergo surgery, or benign, and should be recommended to follow-up. The emergence of precision medicine has offered a valuable solution for this problem, with four tests currently available for the molecular diagnosis of indeterminate cytologies. However, efforts to critically analyze the quality of the accumulated evidence are scarce. This systematic review and meta-analysis is aimed to contribute to a better knowledge about the four available molecular tests, their technical characteristics, clinical performance, and ultimately to help clinicians to make better decisions to provide the best care options possible. For this purpose, we address three critical topics: (i) the proper theoretical accuracy, considering the intended clinical use of the test (rule-in vs rule-out) and the impact on clinical decisions; (ii) the quality of the evidence reported for each test (iii) and how accurate and effective have the tests proved to be after their clinical use. Together with the upcoming evidence, this work provides significant and useful information for healthcare system decision-makers to consider the use of molecular testing as a public health need, avoiding unnecessary surgical risks and costs.

scholarly journals An indeterminate nodule in the cirrhotic liver discovered by surveillance imaging is a prelude to malignancy

2014 ◽  
Vol 110 (8) ◽  
pp. 967-969 ◽  
Author(s):  
Eliza W. Beal ◽  
Scott Albert ◽  
Megan McNally ◽  
Lawrence A. Shirley ◽  
James Hanje ◽  
...  
Keyword(s):  
Cirrhotic Liver ◽  
Indeterminate Nodule ◽  
Surveillance Imaging

scholarly journals MON-511 Are Thyroid Nodule Fine Needle Aspiration Washings an Acceptable Alternative to Dedicated Fine Needle Aspirates When Obtaining Tissue Samples for Genetic Expression Classifier Analysis?

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Maritza Elide Carrillo ◽  
Renil Rodriguez
Keyword(s):  
Clinical Practice ◽  
Thyroid Nodule ◽  
Sample Size ◽  
Thyroid Nodules ◽  
Tissue Samples ◽  
Genetic Expression ◽  
Fine Needle ◽  
Acceptable Alternative ◽  
Indeterminate Nodule ◽  
Undetermined Significance

Abstract The Bethesda System for Reporting Thyroid Cytopathology stratifies thyroid nodules by risk associations. Approximately 15-30% of fine needle aspirations (FNA) fall into an indeterminate criteria, and two thirds are found to be surgically benign leading to unnecessary surgeries (1). Veracyte Inc. developed the Afirma gene expression classifier (GEC) to identify whether an indeterminate nodule had benign expression, with a NPV of 95% for AUS/FLUS (Atypia of undetermined significance/ follicular lesion of undetermined significance) nodules making it an excellent way to rule out malignancy (2). The goal of testing for thyroid cytology includes avoiding unnecessary surgery in benign nodules and identifying high risk from low risk lesions. The Afirma validation landmark study aided in the commercial approval for use in clinical practice after showing a 95.1% sample sufficiency when 2 dedicated samples were obtained in addition to the 3-5 FNA samples for cytology, but our institutional practice habits may suggest other techniques may be valid as well (2). We aimed to assess the rate of sufficient sample for GEC based on FNA washings at our institution, without using dedicated FNA for GEC. Data was gathered from all indeterminate nodule FNA washings referred for Afirma GEC from January 2015 through December 2017. Samples were determined on Afirma report as benign, suspicious, or insufficient and rate of insufficiency was determined. 95 indeterminate nodule FNA washings were sent for Afirma GEC. 93 (97.9%) samples were sufficient for RNA, with 47 (49.5%) reported as suspicious and 46 (48.4%) reported as benign, 2 (2.1%) samples were insufficient for RNA. 97.9% of our samples were sufficient when using FNA washings alone. Our experience suggests that the technique used at our clinical practice is an acceptable alternative to using an additional 2 passes for dedicated samples in collecting RNA for Afirma GEC. This method decreases need for call back for a second biopsy, is less time consuming, and potentially more cost effective. Limitations in our study are a small sample size limited to a single institution, differing number of FNA washings per total passes among practitioners, and variability of experience between providers. In conclusion thyroid nodule FNA washings are an acceptable alternative to dedicated FNA when obtaining tissue samples for genetic expression classifier analysis. Further studies with a larger sample size across different institutions are necessary. 1.Prathima S, Thyroid Research and Practice, January-April 2016; Vol 13: Issue 1; p 9-14 Yang SE et al, Cancer Cytopathology, February 2016, p 100-109 2. Alexander EK. Kennedy GC. Baloch ZW.et al. Preoperative diagnosis of benign thyroid nodules with indeterminate cytology. N Engl J Med. 2012;367(8):705–715.

scholarly journals Auxin and nitric oxide control indeterminate nodule formation

2007 ◽  
Vol 7 (1) ◽  
pp. 21 ◽  
Author(s):  
Youry Pii ◽  
Massimo Crimi ◽  
Giorgia Cremonese ◽  
Angelo Spena ◽  
Tiziana Pandolfini
Keyword(s):  
Nitric Oxide ◽  
Nodule Formation ◽  
Indeterminate Nodule

scholarly journals Sinorhizobium fredii HH103 rkp-3 Genes Are Required for K-Antigen Polysaccharide Biosynthesis, Affect Lipopolysaccharide Structure and Are Essential for Infection of Legumes Forming Determinate Nodules

2012 ◽  
Vol 25 (6) ◽  
pp. 825-838 ◽  
Author(s):  
Isabel Margaret ◽  
Juan C. Crespo-Rivas ◽  
Sebastián Acosta-Jurado ◽  
Ana M. Buendía-Clavería ◽  
María T. Cubo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Cajanus Cajan ◽  
Sinorhizobium Fredii ◽  
Polysaccharide Biosynthesis ◽  
Indeterminate Nodule ◽  
Indeterminate Nodules ◽  
Pseudaminic Acid ◽  
Determinate Nodule ◽  
Static Conditions ◽  
K Antigen

The Sinorhizobium fredii HH103 rkp-3 region has been isolated and sequenced. Based on the similarities between the S. fredii HH103 rkpL, rkpM, rkpN, rkpO, rkpP, and rkpQ genes and their corresponding orthologues in Helicobacter pylori, we propose a possible pathway for the biosynthesis of the S. fredii HH103 K-antigen polysaccharide (KPS) repeating unit. Three rkp-3 genes (rkpM, rkpP, and rkpQ) involved in the biosynthesis of the HH103 KPS repeating unit (a derivative of the pseudaminic acid) have been mutated and analyzed. All the rkp-3 mutants failed to produce KPS and their lipopolysaccharide (LPS) profiles were altered. These mutants showed reduced motility and auto-agglutinated when early-stationary cultures were further incubated under static conditions. Glycine max, Vigna unguiculata (determinate nodule–forming legumes), and Cajanus cajan (indeterminate nodules) plants inoculated with mutants in rkpM, rkpQ, or rkpP only formed pseudonodules that did not fix nitrogen and were devoid of bacteria. In contrast, another indeterminate nodule–forming legume, Glycyrrhiza uralensis, was still able to form some nitrogen-fixing nodules with the three S. fredii HH103 rifampicin-resistant rkp-3 mutants tested. Our results suggest that the severe symbiotic impairment of the S. fredii rkp-3 mutants with soybean, V. unguiculata, and C. cajan is mainly due to the LPS alterations rather than to the incapacity to produce KPS.

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