Evolution of nylon oligomer degrading enzyme based on high-resolution crystal structure analysis

Accurate crystal structure analysis at high temperatures is an important challenge in science and technology. A new electric furnace for the measurement of high-resolution (δd/d= 0.03%) synchrotron radiation powder diffraction profiles from materials at high temperatures (up to 1900 K in air) has been designed and fabricated. This furnace consists of a ceramic refractory with MoSi2heaters, an aluminium body cooled by flowing water, and a sample stage with a spinner and a controller for sample-height adjustment.In situsynchrotron powder diffraction measurement for a calcium titanate perovskite specimen at 1674 K has been performed using the furnace at beamline 3A of the Photon Factory. The electron density distribution of the cubic perovskite at 1674 K was successfully obtained using a combination of Rietveld refinement, the maximum-entropy method (MEM) and MEM-based pattern-fitting techniques. The Ti atoms exhibit covalent bonding with the O atoms in the cubic CaTiO3perovskite at this temperature, while the Ca atoms are ionic. These results indicate that the new furnace yields high-quality data for accurate crystal structure analysis.

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High Resolution Crystal Structure Analysis of [NiFe] Hydrogenase

Nihon Kessho Gakkaishi ◽

10.5940/jcrsj.57.344 ◽

2015 ◽

Vol 57 (6) ◽

pp. 344-349

Author(s):

Hideaki OGATA

Keyword(s):

Crystal Structure ◽

High Resolution ◽

Structure Analysis ◽

Crystal Structure Analysis

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Cyanidioschyzon merolae ferredoxin: A high resolution crystal structure analysis and its thermal stability

FEBS Letters ◽

10.1016/j.febslet.2011.03.020 ◽

2011 ◽

Vol 585 (9) ◽

pp. 1299-1302 ◽

Cited By ~ 7

Author(s):

Asami Yamaoka ◽

Yukiko Ozawa ◽

Yuko Ueno ◽

Tsukasa Endo ◽

Yukio Morimoto ◽

...

Keyword(s):

Crystal Structure ◽

Thermal Stability ◽

High Resolution ◽

Structure Analysis ◽

Crystal Structure Analysis ◽

Cyanidioschyzon Merolae

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Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation – A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2013.06.029 ◽

2013 ◽

Vol 436 (4) ◽

pp. 736-739 ◽

Cited By ~ 2

Author(s):

Yuko Ueno ◽

Takashi Matsumoto ◽

Akihito Yamano ◽

Takeo Imai ◽

Yukio Morimoto

Keyword(s):

Crystal Structure ◽

Electron Transfer ◽

High Resolution ◽

Point Mutation ◽

Structure Analysis ◽

Crystal Structure Analysis ◽

Cyanidioschyzon Merolae ◽

Sad Phasing

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Structure Elucidation of a Novel Analog of Sildenafil Detected as an Adulterant in a Dietary Supplement Using LC-UV and LC/MS

Journal of AOAC International ◽

10.5740/jaoacint.11-235 ◽

2012 ◽

Vol 95 (4) ◽

pp. 1048-1052 ◽

Cited By ~ 5

Author(s):

Miho Sakamoto ◽

Takako Moriyasu ◽

Keiko Minowa ◽

Kiyoko Kishimoto ◽

Hideo Kadoi ◽

...

Keyword(s):

Crystal Structure ◽

High Resolution ◽

Electrospray Ionization ◽

Dietary Supplement ◽

Structure Analysis ◽

Related Compound ◽

Structure Elucidation ◽

Crystal Structure Analysis ◽

X Ray ◽

Nmr Spectrometry

Abstract A sildenafil-related compound was detected in a dietary supplement marketed as an aphrodisiac. The compound was detected during analysis of the dietary supplement using LC-UV and LC/electrospray ionization-MS. The structure of the compound was established using high resolution MS, NMR spectrometry, and X-ray crystal structure analysis. The compound was identified as 5-(5-((3,5-dimethylpiperazin-1-yl)sulfonyl)-2-ethoxyphenyl)-1-methyl-7-((1-methyl-4-nitro-1H-imidazol-5-yl)thio)-3-propyl-1H-pyrazolo[4,3-d] pyrimidine. Based on this structure, the compound was named nitroprodenafil. The dietary supplement was found to contain 90 mg nitroprodenafil/capsule.

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