ID14 `Quadriga', a Beamline for Protein Crystallography at the ESRF

1998 ◽  
Vol 5 (3) ◽  
pp. 215-221 ◽  
Author(s):  
S. Wakatsuki ◽  
H. Belrhali ◽  
E. P. Mitchell ◽  
W. P. Burmeister ◽  
S. M. McSweeney ◽  
...  
Keyword(s):  
User Interface ◽  
Data Collection ◽  
Graphical User Interface ◽  
Macromolecular Crystallography ◽  
Double Crystal ◽  
X Ray ◽  
Double Crystal Monochromator ◽  
Diamond Crystals ◽  
Ccd Detector ◽  
First Results

The ESRF undulator beamline ID14 `Quadriga' is dedicated to monochromatic macromolecular crystallography. Using two undulators with 23 mm and 42 mm periods and a minimum gap of 16 mm installed on a high-β section, it will provide high-brilliance X-ray beams at around 13.5 keV, as well as a wide tuneability between 6.8 and 40 keV. Based on the Troika concept, this beamline has four simultaneously operating experimental stations: three side stations, EH1, EH2 and EH3, using thin diamond crystals, and an end station, EH4, with a fast-scan double-crystal monochromator. Station EH3 has a κ-diffractometer, and an off-line Weissenberg camera with a large 80 × 80 cm active area combined with a 2048 × 2048 CCD detector. During data collection the image plates are placed and removed by a robot located inside the hutch using a cassette system. After data collection the image plates are scanned with an off-line drum scanner. Station EH4 is designed for MAD applications, including Xe K-edge anomalous experiments, and is equipped with a 2048 × 2048 CCD detector on a pseudo 2θ arm. A common graphical user interface and a database will be available to cover all aspects of data collection, including strategy optimization. First results on the performance of the optics elements and initial crystallographic results are presented.

HAXPES beamline PES-BL14 at the Indus-2 synchrotron radiation source

2018 ◽  
Vol 25 (5) ◽  
pp. 1541-1547 ◽  
Author(s):  
Jagannath ◽  
U. K. Goutam ◽  
R. K. Sharma ◽  
J. Singh ◽  
K. Dutta ◽  
...  
Keyword(s):  
Thin Film ◽  
High Resolution ◽  
Electron Spectroscopy ◽  
High Energy ◽  
Solid Samples ◽  
Double Crystal ◽  
X Ray ◽  
Double Crystal Monochromator ◽  
Ccd Detector ◽  
Scientific Results

The Hard X-ray Photo-Electron Spectroscopy (HAXPES) beamline (PES-BL14), installed at the 1.5 T bending-magnet port at the Indian synchrotron (Indus-2), is now available to users. The beamline can be used for X-ray photo-emission electron spectroscopy measurements on solid samples. The PES beamline has an excitation energy range from 3 keV to 15 keV for increased bulk sensitivity. An in-house-developed double-crystal monochromator [Si (111)] and a platinum-coated X-ray mirror are used for the beam monochromatization and manipulation, respectively. This beamline is equipped with a high-energy (up to 15 keV) high-resolution (meV) hemispherical analyzer with a microchannel plate and CCD detector system with SpecsLab Prodigy and CasaXPS software. Additional user facilities include a thin-film laboratory for sample preparation and a workstation for on-site data processing. In this article, the design details of the beamline, other facilities and some recent scientific results are described.

A method to stabilize the incident X-ray energy for anomalous diffraction measurements

2017 ◽  
Vol 24 (4) ◽  
pp. 781-786
Author(s):  
Wenjia Wang ◽  
Xiaoyun Yang ◽  
Guangcai Chang ◽  
Pengfei An ◽  
Kewen Cha ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Data Collection ◽  
Absorption Edge ◽  
Feedback Loop ◽  
Inflection Point ◽  
Single Crystal Diffraction ◽  
Double Crystal ◽  
Anomalous Diffraction ◽  
X Ray ◽  
Double Crystal Monochromator

A method to calibrate and stabilize the incident X-ray energy for anomalous diffraction data collection is provided and has been successfully used at the single-crystal diffraction beamline 1W2B at the Beijing Synchrotron Radiation Facilities. Employing a feedback loop to control the movement of the double-crystal monochromator, this new method enables the incident X-ray energy to be kept within a 0.2 eV range at the inflection point of the absorption edge.

Protein crystallography beamline (PX-BL21) at Indus-2 synchrotron

2016 ◽  
Vol 23 (2) ◽  
pp. 629-634 ◽  
Author(s):  
Ashwani Kumar ◽  
Biplab Ghosh ◽  
H. K. Poswal ◽  
K. K. Pandey ◽  
Jagannath ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Protein Crystallography ◽  
Heavy Elements ◽  
X Ray Diffraction ◽  
Fluorescence Detector ◽  
Double Crystal ◽  
X Ray ◽  
Double Crystal Monochromator ◽  
Ccd Detector ◽  
Scientific Results

The protein crystallography beamline (PX-BL21), installed at the 1.5 T bending-magnet port at the Indian synchrotron (Indus-2), is now available to users. The beamline can be used for X-ray diffraction measurements on a single crystal of macromolecules such as proteins, nucleic acids and their complexes. PX-BL21 has a working energy range of 5–20 keV for accessing the absorption edges of heavy elements commonly used for phasing. A double-crystal monochromator [Si(111) and Si(220)] and a pair of rhodium-coated X-ray mirrors are used for beam monochromatization and manipulation, respectively. This beamline is equipped with a single-axis goniometer, Rayonix MX225 CCD detector, fluorescence detector, cryogenic sample cooler and automated sample changer. Additional user facilities include a workstation for on-site data processing and a biochemistry laboratory for sample preparation. In this article the beamline, other facilities and some recent scientific results are briefly described.

An inexpensive automatically operated device for the flash annealing of crystals of macromolecules

2008 ◽  
Vol 42 (1) ◽  
pp. 125-128 ◽  
Author(s):  
Thierry Giraud ◽  
Fabien Dobias ◽  
José Gabadinho ◽  
Vicente Rey-Bakaikoa ◽  
Didier Nurizzo ◽  
...  
Keyword(s):  
User Interface ◽  
Graphical User Interface ◽  
Standard Technique ◽  
Life Time ◽  
Crystal Quality ◽  
Macromolecular Crystallography ◽  
X Ray ◽  
Flash Annealing ◽  
Flash Cooling ◽  
Control Cabin

In order to prolong the life-time of crystals in an X-ray beam, modern macromolecular crystallography is mainly performed using cryogenically cooled samples. However, in many cases flash cooling can degrade crystal quality if cryoprotection conditions are not ideal. Annealing or tempering of samples adversely affected by cryocooling can often, at least partially, restore diffraction quality and is now considered a standard technique in macromolecular crystallography. A small, inexpensive, automatically operated annealing device that is installed on all ESRF macromolecular crystallography beamlines is presented here. The device allows,viathe beamline control graphical user interface mxCuBE, the flash annealing of samples either from the beamline control cabin or by users accessing the beamline remotely.

Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection

2010 ◽  
Vol 66 (8) ◽  
pp. 855-864 ◽  
Author(s):  
Matthew W. Bowler ◽  
Matias Guijarro ◽  
Sebastien Petitdemange ◽  
Isabel Baker ◽  
Olof Svensson ◽  
...  
Keyword(s):  
Data Collection ◽  
Structural Biology ◽  
Graphical User Interface ◽  
Screening Methods ◽  
Biological Macromolecules ◽  
Macromolecular Crystallography ◽  
Large Crystal ◽  
X Ray ◽  
Automated Methods

Crystals of biological macromolecules often exhibit considerable inter-crystal and intra-crystal variation in diffraction quality. This requires the evaluation of many samples prior to data collection, a practice that is already widespread in macromolecular crystallography. As structural biologists move towards tackling ever more ambitious projects, new automated methods of sample evaluation will become crucial to the success of many projects, as will the availability of synchrotron-based facilities optimized for high-throughput evaluation of the diffraction characteristics of samples. Here, two examples of the types of advanced sample evaluation that will be required are presented: searching within a sample-containing loop for microcrystals using an X-ray beam of 5 µm diameter and selecting the most ordered regions of relatively large crystals using X-ray beams of 5–50 µm in diameter. A graphical user interface developed to assist with these screening methods is also presented. For the case in which the diffraction quality of a relatively large crystal is probed using a microbeam, the usefulness and implications of mapping diffraction-quality heterogeneity (diffraction cartography) are discussed. The implementation of these techniques in the context of planned upgrades to the ESRF's structural biology beamlines is also presented.

scholarly journals Hierarchical clustering for multiple-crystal macromolecular crystallography experiments: theccClusterprogram

2017 ◽  
Vol 50 (6) ◽  
pp. 1844-1851 ◽  
Author(s):  
Gianluca Santoni ◽  
Ulrich Zander ◽  
Christoph Mueller-Dieckmann ◽  
Gordon Leonard ◽  
Alexander Popov
Keyword(s):  
Cluster Analysis ◽  
User Interface ◽  
Data Collection ◽  
Graphical User Interface ◽  
Hierarchical Cluster Analysis ◽  
Hierarchical Cluster ◽  
Macromolecular Crystallography ◽  
Crystal Data ◽  
Multiple Functions ◽  
Data Merging

This article describesccCluster, a software providing an intuitive graphical user interface (GUI) and multiple functions to perform hierarchical cluster analysis on multiple crystallographic datasets. The program makes it easier for users to choose, in the case of multi-crystal data collection, those datasets that will be merged together to give good final statistics. It provides a simple GUI to analyse the dendrogram and various options for automated clustering and data merging.

Trace element analysis on Si wafer surfaces by TXRF at the ID32 ESRF undulator beamline

1998 ◽  
Vol 5 (3) ◽  
pp. 1064-1066 ◽  
Author(s):  
Luc Ortega ◽  
Fabio Comin ◽  
Vincenzo Formoso ◽  
Andreas Stierle
Keyword(s):  
Synchrotron Radiation ◽  
Trace Element ◽  
Trace Element Analysis ◽  
Element Analysis ◽  
Double Crystal ◽  
X Ray ◽  
Double Crystal Monochromator ◽  
First Results ◽  
Set Up ◽  
Wave Methods

Synchrotron radiation total-reflection X-ray fluorescence (SR-TXRF) has been applied to the impurity analysis of Si wafers using a third-generation synchrotron radiation undulator source. A lower limit of detectability (LLD) for Ni atoms of 17 fg (1.7 × 108 atoms cm−2) has been achieved with an optical set-up based on an Si(111) double-crystal monochromator and a horizontal sample geometry. These first results are very promising for synchrotron radiation trace element analysis since we estimate that it is possible to lower the LLD by a factor of about 25 by employing appropriate optics and detectors. The use of a crystal monochromator opens new possibilities to perform absorption and scattering experiments (NEXAFS and X-ray standing-wave methods) for chemical and structural analysis of ultratrace elements.

A DOUBLE CRYSTAL MONOCHROMATOR FOR SOFT X-RAY BEAM LINE OF UVSOR

1986 ◽  
Vol 47 (C8) ◽  
pp. C8-135-C8-137
Author(s):  
T. MURATA ◽  
T. MATSUKAWA ◽  
M. MORI ◽  
M. OBASHI ◽  
S.-I. NAO-E ◽  
...  
Keyword(s):  
Beam Line ◽  
Double Crystal ◽  
X Ray ◽  
Double Crystal Monochromator

scholarly journals Development of mobile robot for measuring distance using optical quadrature encoder

2020 ◽  
Vol 18 (2) ◽  
pp. 745
Author(s):  
Madiha Zahari ◽  
NurliyanaAbd Mutalib ◽  
Nurnadia Natasya Affendi ◽  
N. Hashim ◽  
D. A. Hadi ◽  
...  
Keyword(s):  
User Interface ◽  
Data Collection ◽  
Mobile Robot ◽  
Graphical User Interface ◽  
Accurate Result ◽  
Measuring System ◽  
Accuracy And Precision ◽  
Measuring Tape ◽  
Measuring Tool ◽  
Multiple Conditions

<span>This paper describes the design and development of a measuring tool using a mobile robot. At present, contractors are measuring distances using measuring tape which has few limitations. This includes using of another manpower or a marking flag. The Robot Measuring System is designed to measure distances at multiple conditions such as smooth and rough surface. An optical quadrature encoder is used as a sensor to measure the distances while a program is installed in Arduino Uno for reading and data collection. Graphical User Interface (GUI) was created using Android software so that the movement of the robot can be controlled using a smartphone within a Bluetooth range. An experiment was conducted to test the reliability in terms of accuracy and precision. The best accuracy and precision were obtained when the robot speed is at 90 cm/s on the plain tiles, 80 cm/s on the tar road and 90 cm/s on the grass surface. The robot speed needs to be adjusted accordingly based on the surfaces in order to get an accurate result. <br /> This paper proved that the robot measuring system was successfully designed, implemented and analyzed.</span>

An accurate theory of X-ray coplanar multiple SRMS diffractometry

2018 ◽  
Vol 74 (6) ◽  
pp. 673-680 ◽  
Author(s):  
V. G. Kohn
Keyword(s):  
Synchrotron Radiation ◽  
Plane Wave ◽  
Experimental Setup ◽  
Incident Plane Wave ◽  
Instrumental Function ◽  
Multiple Diffraction ◽  
Double Crystal ◽  
X Ray ◽  
Double Crystal Monochromator ◽  
Incident Plane

The article reports an accurate theory of X-ray coplanar multiple diffraction for an experimental setup that consists of a generic synchrotron radiation (SR) source, double-crystal monochromator (M) and slit (S). It is called for brevity the theory of X-ray coplanar multiple SRMS diffractometry. The theory takes into account the properties of synchrotron radiation as well as the features of diffraction of radiation in the monochromator crystals and the slit. It is shown that the angular and energy dependence (AED) of the sample reflectivity registered by a detector has the form of a convolution of the AED in the case of the monochromatic plane wave with the instrumental function which describes the angular and energy spectrum of radiation incident on the sample crystal. It is shown that such a scheme allows one to measure the rocking curves close to the case of the monochromatic incident plane wave, but only using the high-order reflections by monochromator crystals. The case of four-beam (220)(331)({\overline {11}}1) diffraction in Si is considered in detail.

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