Expression, purification, crystallization and preliminary X-ray analysis of theD-alanyl carrier protein DltC fromStaphylococcus epidermidis
2012 ◽
Vol 68
(7)
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pp. 810-812
Keyword(s):
X Ray
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The D-alanyl lipoteichoic acids (D-alanyl LTAs) present in the cell walls of Gram-positive bacteria play crucial roles in autolysis, cation homeostasis and biofilm formation. The alanylation of LTAs requires the D-alanyl carrier protein DltC to transfer D-Ala onto a membrane-associated LTA. Here, DltC fromStaphylococcus epidermidis(SeDltC) was purified and crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.83 Å and belonged to space groupP2, with unit-cell parametersa= 66.26,b = 53.28,c= 88.05 Å, β = 98.22°. The results give a preliminary crystallographic analysis ofSeDltC and shed light on the functional role of DltC in the alanylation of LTAs.
2015 ◽
Vol 71
(9)
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pp. 1156-1160
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2010 ◽
Vol 66
(9)
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pp. 1050-1052
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2012 ◽
Vol 68
(11)
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pp. 1351-1353
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2014 ◽
Vol 70
(11)
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pp. 1560-1562
2014 ◽
Vol 70
(4)
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pp. 485-488
2015 ◽
Vol 71
(11)
◽
pp. 1396-1400
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2014 ◽
Vol 70
(11)
◽
pp. 1566-1569