Crystallization and preliminary X-ray analysis of the C-terminal domain of δ-COP, a medium-sized subunit of the COPI complex involved in membrane trafficking
2012 ◽
Vol 68
(7)
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pp. 829-831
Keyword(s):
X Ray
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Coat protein I (COPI) is a protein complex composed of seven subunits that mediates retrograde transport of proteins and lipids from thecis-Golgi network to the endoplasmic reticulum and intra-Golgi membranes. The medium-sized δ subunit of COPI (δ-COP) is a 57 kDa protein with a C-terminal domain (CTD) and an N-terminal longin domain. Here, the δ-COP CTD was successfully cloned, purified and crystallized. Diffraction data were collected from native and selenomethionyl crystals of δ-COP CTD to resolutions of 2.60 and 2.30 Å, respectively. Both crystals belonged to space groupP21212, with similar unit-cell parameters. The native crystals had unit-cell parametersa= 100.23,b= 136.77,c = 44.39 Å.
2012 ◽
Vol 76
(4)
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pp. 963-973
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