Expression, crystallization and preliminary crystallographic data analysis of PigF, anO-methyltransferase from the prodigiosin-synthetic pathway inSerratia

2Ch–2N square and hexagon interactions were extensively investigated by the combination of crystallographic data analysis and computational approaches.

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Expression, crystallization and preliminary crystallographic data analysis of PigI, a putativeL-prolyl-AMP ligase from the prodigiosin synthetic pathway inSerratia

Acta Crystallographica Section F Structural Biology Communications ◽

10.1107/s2053230x14005780 ◽

2014 ◽

Vol 70 (5) ◽

pp. 624-627 ◽

Cited By ~ 1

Author(s):

Ning Han ◽

Tingting Ran ◽

Xiangdi Lou ◽

Yanyan Gao ◽

Jianhua He ◽

...

Keyword(s):

Data Analysis ◽

Catalytic Mechanism ◽

Unit Cell ◽

Crystallographic Data ◽

Biosynthesis Pathway ◽

Red Pigment ◽

Asymmetric Unit ◽

Unit Cell Parameters ◽

Solvent Content ◽

Cell Parameters

Prodigiosin, a member of the prodiginines, is a tripyrrole red pigment synthesized bySerratiaand some other microbes. A bifurcated biosynthesis pathway of prodigiosin has been proposed inSerratiain which MBC (4-methoxy-2,2′-bipyrrole-5-carbaldehyde) and MAP (2-methyl-3-N-amyl-pyrrole) are synthesized separately and then condensed by PigC to form prodigiosin. The first step for the synthesis of MBC is the activation of L-proline by PigI, but its catalytic mechanism has remained elusive. To elucidate its mechanism, recombinant PigI was purified and crystallized. Crystals obtained by the sitting-drop method belonged to space groupP1 and diffracted to 2.0 Å resolution, with unit-cell parametersa= 51.2,b= 62.8,c= 91.3 Å, α = 105.1, β = 90.1, γ = 92.2°. Matthews coefficient analysis suggested two molecules in the asymmetric unit, with aVMof 2.6 Å3 Da−1and a solvent content of 52.69%.

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Microchemical imaging in biomedical research

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130225 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1118-1119

Author(s):

P. Ingram

Keyword(s):

Data Analysis ◽

Electron Probe ◽

The Other ◽

X Ray ◽

Cell Element ◽

Physiological Information ◽

Functional States ◽

Elemental Maps ◽

Electron Energy Loss ◽

Secondary Ion

It is well established that unique physiological information can be obtained by rapidly freezing cells in various functional states and analyzing the cell element content and distribution by electron probe x-ray microanalysis. (The other techniques of microanalysis that are amenable to imaging, such as electron energy loss spectroscopy, secondary ion mass spectroscopy, particle induced x-ray emission etc., are not addressed in this tutorial.) However, the usual processes of data acquisition are labor intensive and lengthy, requiring that x-ray counts be collected from individually selected regions of each cell in question and that data analysis be performed subsequent to data collection. A judicious combination of quantitative elemental maps and static raster probes adds not only an additional overall perception of what is occurring during a particular biological manipulation or event, but substantially increases data productivity. Recent advances in microcomputer instrumentation and software have made readily feasible the acquisition and processing of digital quantitative x-ray maps of one to several cells.

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Perceptions of a Collaborative Professional Learning Program: Seeing the “Bigger Picture”

Perspectives of the ASHA Special Interest Groups ◽

10.1044/2019_persp-19-00041 ◽

2020 ◽

Vol 5 (1) ◽

pp. 290-303

Author(s):

P. Charlie Buckley ◽

Kimberly A. Murza ◽

Tami Cassel

Keyword(s):

Data Analysis ◽

Professional Learning ◽

Social Communication ◽

Special Educators ◽

Service Providers ◽

Integrative Approach ◽

Theory Approach ◽

Learning Program ◽

Speech Language Pathologists ◽

Related Service

Purpose The purpose of this study was to explore the perceptions of special education practitioners (i.e., speech-language pathologists, special educators, para-educators, and other related service providers) on their role as communication partners after participation in the Social Communication and Engagement Triad (Buckley et al., 2015 ) yearlong professional learning program. Method A qualitative approach using interviews and purposeful sampling was used. A total of 22 participants who completed participation in either Year 1 or Year 2 of the program were interviewed. Participants were speech-language pathologists, special educators, para-educators, and other related service providers. Using a grounded theory approach (Glaser & Strauss, 1967 ) to data analysis, open, axial, and selective coding procedures were followed. Results Three themes emerged from the data analysis and included engagement as the goal, role as a communication partner, and importance of collaboration. Conclusions Findings supported the notion that educators see the value of an integrative approach to service delivery, supporting students' social communication and engagement across the school day but also recognizing the challenges they face in making this a reality.

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