Approximate robust optimization of time-periodic stationary states with application to biochemical processes

Author(s):  
Boris Houska ◽  
Filip Logist ◽  
Jan Van Impe ◽  
Moritz Diehl
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.


2018 ◽  
Vol E101.B (3) ◽  
pp. 772-784 ◽  
Author(s):  
Bimal CHANDRA DAS ◽  
Satoshi TAKAHASHI ◽  
Eiji OKI ◽  
Masakazu MURAMATSU

2002 ◽  
Author(s):  
Antonio Jose Silveiro Rodrigo ◽  
Jose Paulo Barbosa Mota ◽  
Esteban Saatdjian

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