Fast Start-Up of A2N Denitrifying Phosphorus and Nitrogen Removal System

Author(s):  
Li Yafeng ◽  
Qin Yamin ◽  
Xia Peng
2020 ◽  
Vol 27 (32) ◽  
pp. 40483-40494
Author(s):  
Yaqi Xie ◽  
Chuanyi Zhang ◽  
Limei Yuan ◽  
Qieyuan Gao ◽  
Hai Liang ◽  
...  

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.


2012 ◽  
Vol 610-613 ◽  
pp. 1454-1458
Author(s):  
Ming Fen Niu ◽  
Hong Jing Jiao ◽  
Li Xu ◽  
Yan Yu ◽  
Jian Wei

A2N is two-sludge system, by using the method that first bringing up the cultivation of denitrifying phosphorus removing bacteria (DPB) and nitrification biofilm separately then connecting them, which can start up A2N system successfully. Nitrification biofilm was cultivated in a sequencing batch reactor (SBR). After 30 days, NH4+-N effluent concentration steadily stayed below 0.5mg·L-1.In another SBR, the activated sludge for the enrichment of DPB is from the anaerobic tank, which was firstly operated under anaerobic/aerobic (A/O) condition. After 20 days, PAOs was successfully enriched. Then, the activated sludge was conducted under anaerobic/anoxic/aerobic (A/A/O) condition, maintaining the anaerobic time, gradually increased anoxic time and induced aerobic time. After 30 days DPB was successfully enriched, two phases totally take 50 days. The removal efficiency of total nitrogen and phosphorus are above 85 % and 95 %, so that A2N system was started up successfully.


2015 ◽  
Vol 73 (3) ◽  
pp. 535-542 ◽  
Author(s):  
Yangfan Deng ◽  
Xiaoling Zhang ◽  
Ying Miao ◽  
Bo Hu

In this study, a laboratory-scale sequencing biofilm batch reactor (SBBR) was employed to explore a fast start-up of completely autotrophic nitrogen removal over nitrite (CANON) process. Partial nitrification was achieved by controlling free ammonia concentration and operating at above 30 °C; then the reactor was immediately operated with alternating periods of aerobiosis and anaerobiosis to start the anammox process. The CANON process was successfully achieved in less than 50 d, and the total-nitrogen removal efficiency and the nitrogen removal rate were 81% and 0.14 kg-N m−3 d−1 respectively. Afterwards, with the increasing of ammonium loading rate a maximum nitrogen removal rate of 0.39 kg-N m−3 d−1 was achieved on day 94. DNA analysis showed that ‘Candidatus Brocadia’ was the dominant anammox species and Nitrosomonas was the dominant aerobic ammonium-oxidizing bacteria in the CANON reactor. This study revealed that due to shortening the persistent and stable nitrite accumulation period the long start-up time of the CANON process can be significantly reduced.


1996 ◽  
Vol 33 (12) ◽  
pp. 109-116 ◽  
Author(s):  
Ulf Nyberg ◽  
Bengt Andersson ◽  
Henrik Aspegren

Methanol and ethanol have been used for three years as external carbon sources in a nutrient removal system based on pre-precipitation and post-denitrification in a single sludge activated sludge plant. Based on these long-term experiences it has been shown that the nitrogen standards of 8 mg N/l in the effluent wastewater can be met with both carbon sources. The process entails operational flexibility and the possibility to optimize the nitrogen removal due to seasonal variations in influent wastewater characteristics. Very high specific nitrate utilization rates were measured in the system with the use of external carbon sources. Rates of around 10 mg N/g VSS.h were reached with ethanol and around 3 mg N/g VSS.h with methanol. These rates were much higher than experienced in a pre-denitrification system with the use of the influent organic material as carbon source for denitrification. A start-up with the addition of ethanol led to a direct response of the system while a start-up with methanol resulted in a much longer adaptation period before full effect of the carbon source added was reached.


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