Introduction. Topicality of the study is associated with necessity of physiologic, hygienic and ergonomic basis for methods evaluating tolerance of individual protective means.Objective is to study possible use of contemporary facilities and investigation methods for functional state of humans and their performance within physiologic, hygienic and ergonomic assessment of individual protective means.Methods. The authors used analytic methods and considered methodic issues connected with evaluation of human functional state and performance.Results. Findings are that present regulation basis evaluating influence of individual protective means on human functional state does not consider contemporary novelties in medical equipment, is outdated and requires improvement. The authors justified selection of methods that improve approaches to evaluation of individual protective means in simulated and real industrial conditions. Evidences are that medical and biologic evaluation of individual protective means should study functional state of locomotory system, cardio-respiratory and nervous systems. Ergospirometry is the most informative method to evaluate functional state of cardio-respiratory system, “movements capture” by inertial sensors is the most applicable method to evaluate influence of individual protective means on workers’ mobility, and stabilometry can add existing methods evaluating influence of individual protective means on locomotory and nervous systems.Conclusions. The studies performed can serve as a basis for improvement of prospective methods of physiologic, hygienic and ergonomic evaluation of individual protective means.