Synthesis of Computer Generated Holograms Using Layer-Based Method and Perspective Projection Images

2020 ◽  
Author(s):  
Vincent Brac de la Perriere ◽  
Valter Drazic ◽  
Didier Doyen ◽  
Arno Schubert
Keyword(s):  
Perspective Projection ◽  
Projection Images ◽  
Computer Generated Holograms

scholarly journals Color computer-generated holograms from projection images

2004 ◽  
Vol 12 (11) ◽  
pp. 2487 ◽  
Author(s):  
Yusuke Sando ◽  
Masahide Itoh ◽  
Toyohiko Yatagai
Keyword(s):  
Projection Images ◽  
Computer Generated Holograms ◽  
Color Computer

Imaging of platinum-shadowed macromolecular complexes in dark field

1984 ◽  
Vol 42 ◽  
pp. 146-147
Author(s):  
D.W. Andrews ◽  
F.P. Ottensmeyer
Keyword(s):  
Thin Film ◽  
Three Dimensional ◽  
Average Diameter ◽  
Dark Field ◽  
Bright Field ◽  
Field Mode ◽  
Macromolecular Complexes ◽  
Average Mass ◽  
Topological Features ◽  
Projection Images

Shadowing with heavy metals has been used for many years to enhance the topological features of biological macromolecular complexes. The three dimensional features present in directionaly shadowed specimens often simplifies interpretation of projection images provided by other techniques. One difficulty with the method is the relatively large amount of metal used to achieve sufficient contrast in bright field images. Thick shadow films are undesirable because they decrease resolution due to an increased tendency for microcrystalline aggregates to form, because decoration artefacts become more severe and increased cap thickness makes estimation of dimensions more uncertain.The large increase in contrast provided by the dark field mode of imaging allows the use of shadow replicas with a much lower average mass thickness. To form the images in Fig. 1, latex spheres of 0.087 μ average diameter were unidirectionally shadowed with platinum carbon (Pt-C) and a thin film of carbon was indirectly evaporated on the specimen as a support.

Semi-automated methods for 3-D reconstruction of macromolecular complexes

1995 ◽  
Vol 53 ◽  
pp. 42-43
Author(s):  
B. Carragher ◽  
M. Whittaker
Keyword(s):  
Three Dimensional ◽  
Time Saving ◽  
Macromolecular Complexes ◽  
Symmetry Properties ◽  
Dimensional Reconstruction ◽  
Experienced Operator ◽  
Projection Images ◽  
The Individual ◽  
Natural Symmetry

Techniques for three-dimensional reconstruction of macromolecular complexes from electron micrographs have been successfully used for many years. These include methods which take advantage of the natural symmetry properties of the structure (for example helical or icosahedral) as well as those that use single axis or other tilting geometries to reconstruct from a set of projection images. These techniques have traditionally relied on a very experienced operator to manually perform the often numerous and time consuming steps required to obtain the final reconstruction. While the guidance and oversight of an experienced and critical operator will always be an essential component of these techniques, recent advances in computer technology, microprocessor controlled microscopes and the availability of high quality CCD cameras have provided the means to automate many of the individual steps.During the acquisition of data automation provides benefits not only in terms of convenience and time saving but also in circumstances where manual procedures limit the quality of the final reconstruction.

scholarly journals Occlusion Culling for Wide-Angle Computer-Generated Holograms Using Phase Added Stereogram Technique

Photonics ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 298
Author(s):  
Juan Martinez-Carranza ◽  
Tomasz Kozacki ◽  
Rafał Kukołowicz ◽  
Maksymilian Chlipala ◽  
Moncy Sajeev Idicula
Keyword(s):  
Point Sources ◽  
Wide Angle ◽  
Computer Generated Holograms ◽  
Propagation Methods ◽  
Propagation Technique ◽  
3D Scene ◽  
Occlusion Culling ◽  
Fourier Type ◽  
Cloud Of Points

A computer-generated hologram (CGH) allows synthetizing view of 3D scene of real or virtual objects. Additionally, CGH with wide-angle view offers the possibility of having a 3D experience for large objects. An important feature to consider in the calculation of CGHs is occlusion between surfaces because it provides correct perception of encoded 3D scenes. Although there is a vast family of occlusion culling algorithms, none of these, at the best of our knowledge, consider occlusion when calculating CGHs with wide-angle view. For that reason, in this work we propose an occlusion culling algorithm for wide-angle CGHs that uses the Fourier-type phase added stereogram (PAS). It is shown that segmentation properties of the PAS can be used for setting efficient conditions for occlusion culling of hidden areas. The method is efficient because it enables processing of dense cloud of points. The investigated case has 24 million of point sources. Moreover, quality of the occluded wide-angle CGHs is tested by two propagation methods. The first propagation technique quantifies quality of point reproduction of calculated CGH, while the second method enables the quality assessment of the occlusion culling operation over an object of complex shape. Finally, the applicability of proposed occlusion PAS algorithm is tested by synthetizing wide-angle CGHs that are numerically and optically reconstructed.

scholarly journals Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3652
Author(s):  
Cory Juntunen ◽  
Isabel M. Woller ◽  
Yongjin Sung
Keyword(s):  
Fourier Transform ◽  
3D Imaging ◽  
Optical Tomography ◽  
Three Dimensional ◽  
High Spectral Resolution ◽  
Functional Information ◽  
Fluorescent Beads ◽  
Different Depths ◽  
Projection Images ◽  
Imaging Performance

Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.

scholarly journals Computer-Generated Hologram Based on Reference Light Multiplexing for Holographic Display

2021 ◽  
Vol 11 (16) ◽  
pp. 7199
Author(s):  
Dapu Pi ◽  
Juan Liu
Keyword(s):  
Image Quality ◽  
Numerical Simulations ◽  
Light Wave ◽  
Information Capacity ◽  
Improve Image Quality ◽  
Multiple Images ◽  
Holographic Display ◽  
Light Waves ◽  
Computer Generated Holograms ◽  
Holographic Displays

In this article, we propose a reference light wave multiplexing scheme to increase the information capacity of computer-generated holograms. The holograms were generated by different reference light waves and superimposed together as a multiplexed hologram. A modified Gerchberg–Saxton algorithm was used to improve image quality, and different images could be reconstructed when the multiplexed hologram was illuminated by corresponding reference light waves. We performed both numerical simulations and optical experiments to demonstrate the feasibility of the proposed scheme. Numerical simulations showed that the proposed method could reconstruct multiple images successfully by a single multiplexed hologram and optical experiments are consistently good with numerical simulations. It is expected that the proposed method has great potential to be widely applied in holographic displays in the future.

Design and Fabrication of Computer Generated Holograms for Fresnel Domain Lithography

2009 ◽  
Author(s):  
José A. Domínguez-Caballero ◽  
Satoshi Takahashi ◽  
Sung Jin (James) Lee ◽  
George Barbastathis
Keyword(s):  
Computer Generated Holograms
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