Measurement on Real-Time Diagnostic of Gastric Tumor Model Using Wireless Endoscopy System

2018 ◽  
Author(s):  
Novietasari Chisnariandini ◽  
Arie Pangesti Aji ◽  
Yudiansyah ◽  
Prita Dewi Mariyam ◽  
Jauharul Fuadi ◽  
...  
Keyword(s):  
Real Time ◽  
Tumor Model ◽  
Gastric Tumor

scholarly journals Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice

2020 ◽  
Vol 19 ◽  
pp. 153601212093496
Author(s):  
Adrian Rosenberg ◽  
Daiki Fujimura ◽  
Ryuhei Okada ◽  
Aki Furusawa ◽  
Fuyuki Inagaki ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Tumor Model ◽  
Therapeutic Effects ◽  
Tumor Perfusion ◽  
Background Ratio

Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment. Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour. Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg−1 and 0.3 mg kg−1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly. Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.

scholarly journals Hydroxysteroid sulfotransferase 2B1 affects gastric epithelial function and carcinogenesis induced by a carcinogenic agent

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Wenting Hong ◽  
Fenghua Guo ◽  
Mingjie Yang ◽  
Dongke Xu ◽  
Ziyan Zhuang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Tumor Model ◽  
Gastric Carcinogenesis ◽  
Akt Signaling ◽  
Gastric Epithelial Cell ◽  
Gastric Epithelium ◽  
Gastric Tumor ◽  
Cholesterol Oxidation ◽  
Carcinogenic Agent ◽  
In The Wild

Abstract Background A healthy gastric mucosal epithelium exhibits tumor-suppressive properties. Gastric epithelial cell dysfunction contributes to gastric cancer development. Oxysterols provided from food or cholesterol oxidation in the gastric epithelium may be further sulfated by hydroxysteroid sulfotransferase 2B1 (SULT2B1), which is highly abundant in the gastric epithelium. However, the effects of SULT2B1 on gastric epithelial function and gastric carcinogenesis are unclear. Methods A mouse gastric tumor model was established using carcinogenic agent 3-methylcholanthrene (3-MCA). A SULT2B1 deletion (SULT2B1−/−) human gastric epithelial line GES-1 was constructed by CRISPR/CAS9 genome editing system. Results The gastric tumor incidence was higher in the SULT2B1−/− mice than in the wild-type (WT) mice. In gastric epithelial cells, adenovirus-mediated SULT2B1b overexpression reduced the levels of oxysterols, such as 24(R/S),25-epoxycholesterol (24(R/S),25-EC) and 27-hydroxycholesterol (27HC). This condition also increased PI3K/AKT signaling to promote gastric epithelial cell proliferation, epithelization, and epithelial development. However, SULT2B1 deletion or SULT2B1 knockdown suppressed PI3K/AKT signaling, epithelial cell epithelization, and wound healing and induced gastric epithelial cell malignant transition upon 3-MCA induction. Conclusions The abundant SULT2B1 expression in normal gastric epithelium might maintain epithelial function via the PI3K/AKT signaling pathway and suppress gastric carcinogenesis induced by a carcinogenic agent.

scholarly journals Real‐time bowel sound analysis using newly developed device in patients undergoing gastric surgery for gastric tumor

JGH Open ◽  
2021 ◽  
Author(s):  
Tsutomu Namikawa ◽  
Sachi Yamaguchi ◽  
Kazune Fujisawa ◽  
Maho Ogawa ◽  
Jun Iwabu ◽  
...  
Keyword(s):  
Real Time ◽  
Gastric Surgery ◽  
Gastric Tumor ◽  
Bowel Sound ◽  
Sound Analysis ◽  
Bowel Sound Analysis

A toolbox for the comprehensive, real-time optimization of plasmonic photothermal therapy demonstrated on an orthotopic renal tumor model

2021 ◽  
Author(s):  
Pablo R. Fernández Esteberena ◽  
Clara Vilches ◽  
María del Mar Martínez Lozano ◽  
Ignacio de Miguel ◽  
Oriol Casanovas ◽  
...  
Keyword(s):  
Real Time ◽  
Photothermal Therapy ◽  
Renal Tumor ◽  
Tumor Model ◽  
Time Optimization ◽  
Real Time Optimization ◽  
Plasmonic Photothermal Therapy

CREATION OF CLINICALLY-DIFFERENTIAL TUMOR MIMIC MODEL USING VASELINE-BASED MATERIALS WITH BARIUM SULFATE FOR THE VALIDATION OF REAL-TIME ULTRASOUND IMAGE-GUIDED LIVER BIOPSY SYSTEM

2016 ◽  
Vol 28 (01) ◽  
pp. 1650003
Author(s):  
Cheng Li ◽  
Jin Yao Teo ◽  
Jiaze Wu ◽  
Apoorva Gogna ◽  
Bien Soo Tan ◽  
...  
Keyword(s):  
Liver Biopsy ◽  
Real Time ◽  
Barium Sulfate ◽  
Ex Vivo ◽  
Ultrasound Image ◽  
Tumor Model ◽  
Palpable Mass ◽  
Image Guided ◽  
In Vivo Experiments

Testing objects are important for the validation of developing biopsy systems. Unfortunately, they are very hard to obtain. Motivated by this issue, the purpose of this study is to develop a technique for the easy creation of a model to simulate tumors of different sizes inside porcine livers, which could be used for ultrasound image-guided liver biopsy amongst other applications, and evaluate its performance by comparing to the more widely-used approaches in-vivo and ex-vivo. In this study, a Vaseline-based tumor model, and a more widely-used agar-based tumor model to provide comparison with the proposed method were created and injected into porcine livers as biopsy targets. The clinician located simulated tumors using real-time 2D imaging under the guidance of a robotic arm to delivery the biopsy in ex-vivo and in-vivo experiments. The results show that the optimum tumor model was created from a mixture of Vaseline, glycerol, and barium sulfate which can be easily produced and injected. All Vaseline-based simulated tumors were of solid, palpable mass on gross examination, and ultrasound imaging revealed clearly visible lesions. The clinician successfully performed ultrasound image guided liver biopsy in all the trials (10/10) in the ex-vivo experiment, and 2 out of 3 trials (2/3) in the in-vivo experiment on this optimum tumor model. We described a novel technique of creating solid liver tumor models that can be used for ultrasound image-guided liver biopsy.

scholarly journals Real-time computed tomography fluoroscopy-guided solitary lung tumor model in a rabbit

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179220 ◽  
Author(s):  
Byeong Hyeon Choi ◽  
Hwan Seok Young ◽  
Yu Hua Quan ◽  
Jiyun Rho ◽  
Jae Seon Eo ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Real Time ◽  
Lung Tumor ◽  
Tumor Model ◽  
Computed Tomography Fluoroscopy

scholarly journals Usefulness of a novel device to divide core needle biopsy specimens in a spatially matched fashion

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Takumi Shiraishi ◽  
Shogo Inui ◽  
Yuta Inoue ◽  
Yumiko Saito ◽  
Hideto Taga ◽  
...  
Keyword(s):  
Real Time ◽  
Needle Biopsy ◽  
Genetic Test ◽  
Tumor Model ◽  
Pathological Diagnosis ◽  
The Novel ◽  
Xenograft Tumor ◽  
Genetical Analysis ◽  
New Device ◽  
Novel Device

Abstract We developed a novel dividing device that can split needle biopsy tissues along longitude axis aiming to achieve definitive molecular-biological and genetical analysis with reference of pathological diagnosis of the side-by-side divided tissue as spatially matched information. The aim of this study was to evaluate the feasibility and potential usefulness of the novel dividing device to provide the appropriate materials for molecular diagnosis. The new device was examined using mouse xenograft tumors. Real-time quantitative PCR and genetic test were performed to evaluate the feasibility and usefulness of the device. All the samples from needle biopsy were successfully divided into two pieces. Quality and quantity from divided samples harbor high enough to perform gene expression analysis (real-time PCR) and genetic test. Using two divided samples obtained from xenograft tumor model by needle biopsy, the % length of xenograft tumor (human origin) was significantly correlated with the % human genomic DNA (p = 0.00000608, r = 0.987), indicating that these divided samples were spatially matched. The novel longitudinally dividing device of a needle biopsy tissue was useful to provide the appropriate materials for molecular-biological and genetical analysis with reference of pathological diagnosis as spatially matched information.

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