A hand-eye calibration method for computer assisted endoscopy

Author(s):  
Wei ◽  
Kumsok Kang ◽  
Huamin Yang ◽  
Yu Miao ◽  
Weili Shi ◽  
...  
2009 ◽  
Author(s):  
András Lassó ◽  
Junichi Tokuda ◽  
Siddharth Vikal ◽  
Clare M Tempany ◽  
Nobuhiko Hata ◽  
...  

Various frameworks and toolkits have been proposed for rapid development of computer assisted intervention (CAI) software. In this paper, we investigate how the open-source 3D Slicer application framework can be used for this purpose. We defined common requirements for CAI software to evaluate and enhance 3D Slicer for interventional applications in general. 3D Slicer is found to be an appropriate end-user deployment environment for CAI software, as its built-in functions fulfill many requirements and missing functionalities can be conveniently added. We describe the implementation of a CAI software based on extending core 3D Slicer functions. Three enhancements are described in detail: the management of workflow, DICOM image transfer, and multiple views. The resulting software fulfills general CAI requirements and supports two different MRI-guided prostate biopsy systems (each with a different imaging mode, robotic manipulator and calibration method), thereby demonstrating the usability of Slicer for implementing different CAI applications.


Author(s):  
Marcelo E. de Oliveira ◽  
Henrique G. Debarba ◽  
Alexandre Lädermann ◽  
Sylvain Chagué ◽  
Caecilia Charbonnier

2018 ◽  
Vol 104 (8) ◽  
pp. S110
Author(s):  
Marcelo de Oliveira ◽  
Alexandre Lädermann ◽  
Henrique Debarba ◽  
Sylvain Chagué ◽  
Philippe Collin ◽  
...  

Sensors ◽  
2021 ◽  
Vol 21 (9) ◽  
pp. 2931
Author(s):  
Yuan Gao ◽  
Yuyun Zhao ◽  
Le Xie ◽  
Guoyan Zheng

In the medical field, guidance to follow the surgical plan is crucial. Image overlay projection is a solution to link the surgical plan with the patient. It realizes augmented reality (AR) by projecting computer-generated image on the surface of the target through a projector, which can visualize additional information to the scene. By overlaying anatomical information or surgical plans on the surgery area, projection helps to enhance the surgeon’s understanding of the anatomical structure, and intuitively visualizes the surgical target and key structures of the operation, and avoid the surgeon’s sight diversion between monitor and patient. However, it still remains a challenge to project the surgical navigation information on the target precisely and efficiently. In this study, we propose a projector-based surgical navigation system. Through the gray code-based calibration method, the projector can be calibrated with a camera and then be integrated with an optical spatial locator, so that the navigation information of the operation can be accurately projected onto the target area. We validated the projection accuracy of the system through back projection, with average projection error of 3.37 pixels in x direction and 1.51 pixels in y direction, and model projection with an average position error of 1.03 ± 0.43 mm, and carried out puncture experiments using the system with correct rate of 99%, and qualitatively analyzed the system’s performance through the questionnaire. The results demonstrate the efficacy of our proposed AR system.


Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.


Author(s):  
A.M. Jones ◽  
A. Max Fiskin

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.


Author(s):  
Beverly L. Giammara ◽  
Jennifer S. Stevenson ◽  
Peggy E. Yates ◽  
Robert H. Gunderson ◽  
Jacob S. Hanker

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.


Author(s):  
Rudolf Oldenbourg

The recent renaissance of the light microsope is fueled in part by technological advances in components on the periphery of the microscope, such as the laser as illumination source, electronic image recording (video), computer assisted image analysis and the biochemistry of fluorescent dyes for labeling specimens. After great progress in these peripheral parts, it seems timely to examine the optics itself and ask how progress in the periphery facilitates the use of new optical components and of new optical designs inside the microscope. Some results of this fruitful reflection are presented in this symposium.We have considered the polarized light microscope, and developed a design that replaces the traditional compensator, typically a birefringent crystal plate, with a precision universal compensator made of two liquid crystal variable retarders. A video camera and digital image processing system provide fast measurements of specimen anisotropy (retardance magnitude and azimuth) at ALL POINTS of the image forming the field of view. The images document fine structural and molecular organization within a thin optical section of the specimen.


Author(s):  
M Wessendorf ◽  
A Beuning ◽  
D Cameron ◽  
J Williams ◽  
C Knox

Multi-color confocal scanning-laser microscopy (CSLM) allows examination of the relationships between neuronal somata and the nerve fibers surrounding them at sub-micron resolution in x,y, and z. Given these properties, it should be possible to use multi-color CSLM to identify relationships that might be synapses and eliminate those that are clearly too distant to be synapses. In previous studies of this type, pairs of images (e.g., red and green images for tissue stained with rhodamine and fluorescein) have been merged and examined for nerve terminals that appose a stained cell (see, for instance, Mason et al.). The above method suffers from two disadvantages, though. First, although it is possible to recognize appositions in which the varicosity abuts the cell in the x or y axes, it is more difficult to recognize them if the apposition is oriented at all in the z-axis—e.g., if the varicosity lies above or below the neuron rather than next to it. Second, using this method to identify potential appositions over an entire cell is time-consuming and tedious.


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