A Study on Safety of Pet Food Products

2021 ◽  
Author(s):  
S.L. Mak ◽  
M.Y.T. Wu ◽  
W.F. Tang ◽  
C.H. Li ◽  
H. Wang ◽  
...  
Keyword(s):  
Food Products ◽  
Pet Food

scholarly journals Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2875
Author(s):  
Laura Preckel ◽  
Claudia Brünen-Nieweler ◽  
Grégoire Denay ◽  
Henning Petersen ◽  
Margit Cichna-Markl ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Animal Species ◽  
Meat Product ◽  
Food Products ◽  
Attractive Alternative ◽  
Pet Food ◽  
Food Authentication ◽  
Dna Metabarcoding ◽  
Reference Samples

The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species.

Evaluating Pediococcus acidilactici and Enterococcus faecium NRRL B-2354 as Thermal Surrogate Microorganisms for Salmonella for In-Plant Validation Studies of Low-Moisture Pet Food Products

2015 ◽  
Vol 78 (5) ◽  
pp. 934-939 ◽  
Author(s):  
ERDOGAN CEYLAN ◽  
DERRICK A. BAUTISTA
Keyword(s):  
Enterococcus Faecium ◽  
Thermal Inactivation ◽  
Food Product ◽  
Food Products ◽  
Pediococcus Acidilactici ◽  
Pet Food ◽  
Death Time ◽  
Salmonella Serovars ◽  
D Values ◽  
Thermal Death Time

Pediococcus acidilactici ATCC 8042 and Enterococcus faecium NRRL B-2354 were investigated as potential surrogates for Salmonella serovars using thermal death time kinetics in products such as dry pet foods. The D-values of P. acidilactici ATCC 8042, E. faecium NRRL B-2354, and a cocktail of seven Salmonella serovars associated with low-moisture products were determined in a preservative-free dry pet food product at moisture levels of 9.1, 17.9, and 27.0% and heated between 76.7 and 87.8°C. The D-values were calculated by least squares linear regression. The D-values of P. acidilactici ATCC 8042 were higher than those for the Salmonella serovar cocktail but lower than those for E. faecium NRRL 2354. At 9.1% moisture, D-values of 6.54, 11.51, and 11.66 min at 76.7°C, 2.66, 3.22, and 4.08 min at 82.2°C, and 1.07, 1.29, and 1.69 min at 87.8°C were calculated for Salmonella serovars, P. acidilactici ATCC 8042, and E. faecium NRRL B-2354, respectively. The data suggest that the thermal inactivation characteristics of P. acidilactici ATCC 8042 can be utilized as a surrogate to predict the response of Salmonella in dry pet food products that are thermally processed at <90°C.

scholarly journals Melamine, Asam Suanurat dan Melamin-Sianurat: Kaitan dengan Penyakit Saluran Perkencingan Hewan

2019 ◽  
Vol 37 (2) ◽  
pp. 193
Author(s):  
Yanuartono Yanuartono ◽  
Alfarisa Nururrozia ◽  
Soedarmanto Indarjulianto ◽  
Hary Purnamaningsih ◽  
Slamet Rahardjo
Keyword(s):  
Renal Failure ◽  
Cyanuric Acid ◽  
Animal Feed ◽  
Case Reports ◽  
Food Products ◽  
Nitrogen Compounds ◽  
Pet Food ◽  
Protein Food ◽  
Melamine Cyanurate ◽  
Food And Feed

Numerous cases of acute renal failure in dogs and cats from 2004-2007 were associated with the ingestion of dog and cat pet food products and the suspected contaminant was identified as melamine and its derivatives such as cyanuric acid and melamine cyanurate. Melamine is a chemical used primarily for the manufacture of plastics, coatings, commercial filters, adhesives and compounds in the plate-making industry and kitchen appliance.But then these compounds have been abused to increase the content of nitrogen compounds in human food products and animal feed products, respectively. Increasing the content of nitrogen compounds from melamine aims to increase the value of false protein food and feed. Melamine basically has a wide security limit so it is relatively non-toxic if consumed by humans and animals. However, many studies and case reports show that melamine can cause damage to the kidneys especially if present together with cyanuric acid in the body.This paper aims to provide a simple overview and discussion in cases of damage to the urinary tract in dogs and cats that are likely caused by toxicity of melamine and its derivatives are likely to have occurred in Indonesia. 

Sensory evaluation of pet food products

2022 ◽  
pp. 313-329
Author(s):  
Julien Rogues ◽  
Erika Csoltova ◽  
Claire Larose-Forges ◽  
Emira Mehinagic
Keyword(s):  
Sensory Evaluation ◽  
Food Products ◽  
Pet Food

scholarly journals NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fabio Palumbo ◽  
Francesco Scariolo ◽  
Alessandro Vannozzi ◽  
Gianni Barcaccia
Keyword(s):  
Ad Hoc ◽  
Food Products ◽  
Coi Gene ◽  
Pet Food ◽  
Discrimination Power ◽  
Gene Target ◽  
Traceability System ◽  
Food And Feed ◽  
Food Market ◽  
Generation Sequencing

Abstract Pet food industry has grown considerably in the last few years and it is expected to continue with this rate. Despite the economic impact of this sector and the consumer concerns for the increasing number of food and feed adulteration cases, few studies have been published on mislabelling in pet foods. We therefore investigated the capability of a next generation sequencing-based mini-barcoding approach to identify animal species in pet food products. In a preliminary analysis, a 127 bp fragment of the COI gene was tested on both individual specimens and ad hoc mixed fresh samples used as testers, to evaluate its discrimination power and primers effectiveness. Eighteen pet food products of different price categories and forms available on the market (i.e. kibbles, bites, pâté and strips) were analysed through an NGS approach in biological replicates. At least one of the species listed in the ingredients was not detected in half of the products, while seven products showed supplementary species in addition to those stated on the label. Due to the accuracy, sensitivity and specificity demonstrated, this method can be proposed as food genetic traceability system to evaluate both the feed and food quality timely along the supply chain.

Validation of the Soleris® Direct Yeast and Mold Method for Semiquantitative Determination of Yeast and Mold in a Variety of Foods

2014 ◽  
Vol 97 (4) ◽  
pp. 1084-1091
Author(s):  
Marcelle Pereault ◽  
Susan Alles ◽  
Oscar Caballero ◽  
Ron Sarver ◽  
Susan McDougal ◽  
...  
Keyword(s):  
Incubation Temperature ◽  
Operating Parameter ◽  
Reference Method ◽  
Food Products ◽  
Threshold Level ◽  
Black Pepper ◽  
Probability Of Detection ◽  
Pet Food ◽  
Threshold Levels ◽  
Multiple Test

Abstract A study was carried out to determine the efficacy of the Soleris® Direct Yeast and Mold (DYM) automated growth-based method for semiquantitative detection of yeast and mold in a variety of food products. A probability of detection (POD) statistical model was used to compare Soleris results at multiple test thresholds (dilutions) with plate counts determined using the U.S. Food and Drug Administration Bacteriological Analytical Manual, Chapter 18, dilution plating procedure. Fourteen naturally contaminated food products were tested, with Soleris testing performed at three or more threshold levels for each food. Using the POD model, the majority of Soleris test results were in statistical agreement with the reference plating procedures. The exceptions included a single threshold level in yogurt, black pepper, dried fruit, and dry pet food, and two levels in nonfat dry milk and saw palmetto powder. In all but one of these instances, the exception being pet food, the statistical disagreement was due to Soleris estimating a higher level of contamination than the reference method. Results of ruggedness testing showed that the Soleris method produced accurate results even when significant variances in a critical operating parameter, incubation temperature, were introduced. Results of the internal and independent laboratory validation studies showed that the Soleris DYM method can be used as an accurate alternative to conventional dilution plating procedures for evaluation of yeast and mold counts at threshold levels, while saving as much as 72 h in analysis time.

Prevalence and Concentration of Arcobacter spp. on Australian Beef Carcasses

2012 ◽  
Vol 75 (8) ◽  
pp. 1479-1482 ◽  
Author(s):  
LESLEY L. DUFFY ◽  
NARELLE FEGAN
Keyword(s):  
Limit Of Detection ◽  
Food Products ◽  
International Commission ◽  
Most Probable Number ◽  
Pet Food ◽  
Emerging Pathogens ◽  
Probable Number ◽  
Beef Carcasses ◽  
Low Levels ◽  
Low Prevalence

The International Commission on Microbiological Specifications for Foods (ICMSF) classified Arcobacter spp. as emerging pathogens in 2002. Arcobacter spp. have been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. A survey was conducted to determine both the prevalence and concentration of Arcobacter spp. on prechill beef carcasses. Surface swab samples were collected from 130 beef carcasses at the end of processing, prior to chilling. The concentration of Arcobacter spp. was determined by a most-probable-number per square centimeter (3 by 3) method with a limit of detection of 0.12 CFU/cm2. Of the 100 carcasses examined from export abattoirs, 20 (20.0%) were contaminated with Arcobacter spp., and 5 of these had quantifiable levels of contamination ranging from 0.12 to 0.31 CFU/cm2. Of the 30 carcasses examined at a pet food abattoir, 25 (83.3%) were contaminated with Arcobacter spp., and 10 of these had quantifiable levels of contamination ranging from 0.12 to 0.95 CFU/cm2. Three species of Arcobacter, A. butzleri, A. cryaerophilus, and A. skirowii, were identified by PCR. Each of the species was present in an approximately equal ratio from export abattoirs. This study demonstrates that slaughter practices at export abattoirs are sufficient to maintain both low prevalence and low levels of contamination of beef carcasses with Arcobacter spp.

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