SIC: an intracerebral radiosensitive probe for in vivo neuropharmacology investigations in small laboratory animals: first prototype design, characterization and in vivo evaluation

2002 ◽  
Author(s):  
F. Pain ◽  
P. Laniece ◽  
R. Mastrippolito ◽  
L. Pinot ◽  
Y. Charon ◽  
...  
Keyword(s):  
Laboratory Animals ◽  
Small Laboratory ◽  
In Vivo Evaluation ◽  
Prototype Design

scholarly journals Design and in vivo evaluation of a microparticulate depot formulation of buprenorphine for veterinary use

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Viktoria Schreiner ◽  
Mattea Durst ◽  
Margarete Arras ◽  
Pascal Detampel ◽  
Paulin Jirkof ◽  
...  
Keyword(s):  
Drug Release ◽  
Prolonged Exposure ◽  
Drug Formulation ◽  
Spherical Particles ◽  
Laboratory Animals ◽  
Attractive Alternative ◽  
Burst Release ◽  
In Vivo Evaluation ◽  
Duration Of Action

Abstract Buprenorphine is a frequently used analgetic agent in veterinary medicine. A major drawback, however, is the short duration of action requiring several daily administrations. We therefore designed a poly-lactic-co-glycolic acid (PLGA) based microparticulate drug formulation for sustained parenteral drug release. Particles were designed to allow for a fast onset of action and a duration of the analgesic effect of at least two days in laboratory mice. Microparticles were produced using a solvent evaporation technique. Release rate was dependent on polymer type and particle size. Spherical particles used for subsequent animal studies had a mean size of 50 µm and contained 4.5% of buprenorphine. Drug release was characterized by an initial burst release of 30% followed by complete release over seven days. In vivo pharmacokinetic experiments in female C57BL/6 J mice confirmed prolonged exposure in plasma and brain tissue and correlated with the pharmacological effect in the hot plate assay or after minor abdominal surgery. No adverse side effects with respect to food and water intake, body weight, local tolerability, or nesting behavior were observed. Our formulation is an attractive alternative to established immediate release formulations. A use for prolonged pain management in laboratory animals is proposed.

Nasal cavity dimensions in guinea pig and rat measured by acoustic rhinometry and fluid-displacement method

2004 ◽  
Vol 96 (6) ◽  
pp. 2109-2114 ◽  
Author(s):  
Sune P. Straszek ◽  
Ole F. Pedersen
Keyword(s):  
Nasal Cavity ◽  
Guinea Pigs ◽  
Acoustic Rhinometry ◽  
Laboratory Animals ◽  
Small Laboratory ◽  
Acoustic Measurements ◽  
Displacement Method ◽  
Fluid Displacement ◽  
Custom Made

The purpose of the study was to measure nasal passageway dimensions in guinea pigs and rats by use of acoustic rhinometry (AR) and by a previously described fluid-displacement method (FDM) (Straszek SP, Taagehoej F, Graff S, and Pedersen OF. J Appl Physiol 95: 635–642, 2003) to investigate the potential of AR in pharmacological research with these animals. We measured the area-distance relationships by AR of nasal cavities postmortem in five guinea pigs (Duncan Hartley, 400 g) and five rats (Wistar, 250 g) by using custom-made equipment scaled for the purpose. Nosepieces were made from plastic pipette tips and either inserted into or glued onto the nostrils. We used liquid perfluorocarbon in the fluid-displacement study, and it was carried out subsequent to the acoustic measurements. We found for guinea pigs that AR measured a mean volume of 98 mm3 (95–100 mm3) (mean and 95% confidence interval) of the first 2 cm of the cavity. FDM measured a mean volume of 146 mm3 (117–175 mm3), meaning that AR only measured 70% (50–90) of the volume by FDM. For rats, the volume from 0 to 2 cm was 58 mm3 (55–61 mm3) by AR and 73 mm3 (60–87 mm3) by FDM, resulting in AR only measuring 83% (66–100%) of volume by FDM (see Table 2 ). We conclude that absolute nasal cavity dimensions are underestimated by AR in guinea pigs and rats. This does not preclude that relative changes may be correctly measured. In vivo trials with AR using rats have not yet been published. The FDM is possibly the most accurate alternative to AR for measurements of the nasal cavity geometry in small laboratory animals, but it can only be used postmortem.

scholarly journals Visualization capabilities of experimental oncological models in small laboratory animals

2018 ◽  
Vol 9 (4) ◽  
pp. 105-112 ◽  
Author(s):  
Valeria A. Pechatnikova ◽  
Alexander P. Trashkov ◽  
Maria A. Zelenenko ◽  
Nikolay A. Verlov ◽  
Grigorii A. Chizh ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Pathological Process ◽  
Nerve Fibers ◽  
Laboratory Animals ◽  
Small Laboratory ◽  
Internal Organs ◽  
Imaging Methods ◽  
Non Invasive ◽  
Long Time

For a long time non-invasive imaging methods have been inaccessible in preclinical practice; their introduction lately has broadened the boundaries of relevant studies and felicitated new approaches to solving fundamental problems. Up-to-date imaging methods constitute an essential component of preclinical and translational biomedical research allowing quick and non-invasive extended representation of structural organization and functional characteristics of pathological processes in vivo. Methods of radiation diagnosis and nuclear magnetic resonance allow to assess the state of bones, soft tissues, internal organs, blood vessels and peripheral nerve fibers in various animals, not only mammals, but also fish, amphibians, reptiles and insects. Multiparametric studies can uniquely localize any anatomical structure or pathological process. However, not all existing techniques are applicable to various oncological models of small laboratory animals.

High-resolution light microscopy of living organs in situ

1995 ◽  
Vol 53 ◽  
pp. 790-791
Author(s):  
R. S. McCuskey
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Laboratory Animals ◽  
Small Laboratory ◽  
Abdominal Incision ◽  
Standard Compound ◽  
Working Distance ◽  
Microscope Stage

Most organs in anesthetized small laboratory animals can be studied in vivo by light microscopy of relatively thin (3-5mm), transilluminated areas of the organ. Thicker areas of the organs in these species, as well as thicker organs of larger animals can be examined only by epi-ilumination. However, the resolution obtainable with epi-ilumination usually is inferior to that realized with transillumination. This paper reviews these methods using the liver as an example of the organ of study. A standard compound trinocular microscope is used which is modified for in vivo microscopy and is equipped for both transillumination and epi-illumination. After the animal is anesthetized, the liver is gently exteriorized through a subcostal, abdominal incision and positioned over a window of optical grade mica or glass on a specially designed, heated microscope stage having provisions for draining irrigation fluids. The window overlies a long working distance condenser. The liver is covered by a piece of Saran or Mylar film which holds it in position and limits movements induced by respiration, the heart and the intestines.

scholarly journals Validation of Сhoice of Laboratory Model for Preclinical Estimation of Medical Protectors Against Bolivian Hemorrhagic Fever

2019 ◽  
Vol 3 (4) ◽  
pp. 319-328
Keyword(s):  
Animal Models ◽  
Guinea Pigs ◽  
Macaca Fascicularis ◽  
Hemorrhagic Fever ◽  
Laboratory Animals ◽  
Laboratory Model ◽  
Small Laboratory ◽  
Quantitative Indices ◽  
Machupo Virus

Th is review is dedicated to the peculiarities of pathogenesis of the experimental Bolivian hemorrhagic fever (BHF) – the disease, caused by Machupo virus (Arenaviridae family). Th e authors come to the conclusion that for carrying out preclinical researches of the medical means of protection (MMP) in vivo on small laboratory animals it is expedient to use guinea pigs, infected with a strain of Chicava or with a variant of Carvallo strain, adapted for these animals. Th e use of guinea pigs as small laboratory animals when studying pathogenesis of the disease caused by Machupo virus allows to carry out statistically reliable defi nition of quantitative indices of an experimental infection and to select medicines for the fi nal stage of preclinical assessment. As arenaviruses block the process of formation of interferon (IFN) in the infected organism, mice, defective by IFN formation, are the perspective animal models for the study of BHF pathogenesis and may be used for the study of attenuated variants of Machupo virus. Th e Javanese macaques (Macaca fascicularis) are the laboratory animals, modeling the pathogenetic manifestations of BHF in humans. Th ey can be used when carrying out the fi nal stages of preclinical assessment of means of medical protection

Investigating the Dopaminergic Synapse In Vivo. II. Molecular Imaging Studies in Small Laboratory Animals

2007 ◽  
Vol 18 (6) ◽  
Author(s):  
Susanne Nikolaus ◽  
Rolf Larisch ◽  
Markus Beu ◽  
Christina Antke ◽  
Konstantin Kley ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Laboratory Animals ◽  
Small Laboratory ◽  
Imaging Studies

Virus safety in xenotransplantation: first exploratory in vivo studies in small laboratory animals and non-human primates

2002 ◽  
Vol 9 (2-4) ◽  
pp. 281-288 ◽  
Author(s):  
V Specke ◽  
H.-J Schuurman ◽  
R Plesker ◽  
C Coulibaly ◽  
M Özel ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Laboratory Animals ◽  
Small Laboratory ◽  
Virus Safety

scholarly journals Synthesis of Some New Acetanilide Derivatives as COX Inhibitors and Assessment of Analgesic/ Anti-Inflammatory Activities

2021 ◽  
Vol 37 (6) ◽  
pp. 1475-1487
Author(s):  
Rajib Kumar Singh ◽  
Arvind Kumar ◽  
Arun K. Mishra
Keyword(s):  
Diclofenac Sodium ◽  
Docking Studies ◽  
Laboratory Animals ◽  
In Vivo Evaluation ◽  
Activity Evaluation ◽  
Physiochemical Parameters ◽  
Cox 2 ◽  
Anti Inflammatory

The purpose of the present research was to synthesize a new series of acetanilide derivatives that would have analgesic and anti-inflammatory properties in laboratory animals (rats). IR spectroscopy, 1HNMR spectroscopy and Mass spectroscopy were used to confirm the structures of freshly synthesised compounds. The goal of the computer analysis of synthesized compounds was to see how similar they were in terms of physicochemical properties. For this, physiochemical parameters were calculated. The result suggested the reasonable physiochemical similarity with diclofenac sodium and Indomethacin. Molecular docking studies showed that the all the test compounds perfectly docked with COX-2 enzyme with all the drug-likeness characteristics. Before start of in-vivo evaluation, in vitro cyclooxygenase (COX) inhibition assays was performed with an aim to evaluate the compounds against the protein target COX-2 which would exhibit their inhibitory activity. The test compounds (C1-C6) were subjected to analgesic activity evaluation by Eddy’s hot plate method and anti-inflammatory activity evaluation by Carageenan induced edema method respectively.

Sign in / Sign up

Export Citation Format

Share Document