Wireless Thermometry for Real-Time Temperature Recording on Thousand-Cell Level

2019 ◽  
Vol 66 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Can Li ◽  
Jianfei Sun ◽  
Qiwei Wang ◽  
Weiguo Zhang ◽  
Ning Gu
Keyword(s):  
Real Time ◽  
Cell Level ◽  
Temperature Recording

Quantitative Detection and Real-Time Monitoring of Endogenous mRNA at the Single Live Cell Level Using a Ratiometric Molecular Beacon

2019 ◽  
Vol 11 (32) ◽  
pp. 28752-28761 ◽  
Author(s):  
Xufei Feng ◽  
Wenjie Kang ◽  
Xuping Wu ◽  
Shouyu Wang ◽  
Fei Liu
Keyword(s):  
Real Time ◽  
Molecular Beacon ◽  
Live Cell ◽  
Quantitative Detection ◽  
Real Time Monitoring ◽  
Cell Level

scholarly journals Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Delphine Aymoz ◽  
Victoria Wosika ◽  
Eric Durandau ◽  
Serge Pelet
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Cell Level

Measurement of Molecular Mobility in Mammalian Cells

2004 ◽  
Author(s):  
Alptekin Aksan ◽  
Mehmet Toner
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Chemical Reaction ◽  
Molecular Mobility ◽  
Mammalian Cells ◽  
Free Water ◽  
Single Cell Level ◽  
Extracellular Water ◽  
Cell Level

Preservation of mammalian cells requires establishing a reversible stasis condition by reducing the intra/extracellular molecular mobility ensuring reduced chemical reaction and deterioration rates. Molecular mobility may be reduced by various techniques. For example, in cryopreservation, mobility within and surrounding the cell is reduced through freezing the free water that constitutes 70–90% of the cell’s composition. In dried-state preservation applied successfully to preserve seeds, pharmacological materials and foodstuff (mimicking the anhydrobiosis phenomenon seen in nature), reduction in molecular mobility is established by removing intra/extracellular water. Certain carbohydrates (such as trehalose and sucrose) can be artificially uploaded into mammalian cells to replace the removed water and to form an intra/extracellular glass. In this research, a fluorescent rotor is utilized to determine the changes in intracellular molecular mobility during carbohydrate uploading of mammalian cells. It was shown that using this technique, it is feasible to make real-time mobility measurements at a single cell level.

Effect of Hydroxyapatite Nanorods on the Fate of Human Adipose‐Derived Stem Cells Assessed In Situ at the Single Cell Level with a High‐Throughput, Real‐Time Microfluidic Chip

Small ◽  
2019 ◽  
Vol 15 (51) ◽  
pp. 1905001 ◽  
Author(s):  
Min Hao ◽  
Jianlong He ◽  
Chunhua Wang ◽  
Chao Wang ◽  
Baojin Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Single Cell Level ◽  
Adipose Derived Stem Cells ◽  
Cell Level

scholarly journals Method for real-time monitoring of protein degradation at the single cell level

BioTechniques ◽  
2007 ◽  
Vol 42 (4) ◽  
pp. 446-450 ◽  
Author(s):  
Lijuan Zhang ◽  
Nadya G. Gurskaya ◽  
Ekaterina M. Merzlyak ◽  
Dmitry B. Staroverov ◽  
Nikolay N. Mudrik ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level

Inkjet Printing-assisted Single-Cell Microarray on a Hydrophobic Surface Chip for Real-Time Monitoring of Enzyme Kinetics at Single-cell Level

Talanta ◽  
2021 ◽  
pp. 122019
Author(s):  
Xuexia Lin ◽  
Feixiang Fang ◽  
Chenjing Wang ◽  
Ranjith Kumar Kankala ◽  
Shufeng Zhou
Keyword(s):  
Enzyme Kinetics ◽  
Single Cell ◽  
Real Time ◽  
Inkjet Printing ◽  
Hydrophobic Surface ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level ◽  
Cell Microarray

scholarly journals Auto-luminescent genetically encoded ratiometric indicator for real-time Ca2+imaging at the single cell level

2011 ◽  
Author(s):  
Kenta Saito ◽  
Kentaro Kobayashi ◽  
Takeharu Nagai
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Cell Level

Reporter gene expression at single-cell level characterized with real-time RT-PCR, chemiluminescence, fluorescence, and electrochemical imaging

FEBS Letters ◽  
2010 ◽  
Vol 584 (18) ◽  
pp. 4000-4008 ◽  
Author(s):  
Hitoshi Shiku ◽  
Daisuke Okazaki ◽  
Junya Suzuki ◽  
Yasufumi Takahashi ◽  
Tatsuya Murata ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Real Time ◽  
Reporter Gene ◽  
Reporter Gene Expression ◽  
Single Cell Level ◽  
Rt Pcr ◽  
Cell Level ◽  
Electrochemical Imaging
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