Reversible Permeabilization of Cancer Cells by High Sub-Microsecond Magnetic Field

The cytotoxicity of magnetic nanoparticles-conjugated polymeric micelles encapsulated with an anticancer drug on cancer cells was enhanced by the synergistic effect of heat and the rapid release of the drug under an alternating magnetic field.

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A novel magnetic field generating machine with a wide range of tunable parameters to study in vitro cells

10.21203/rs.3.rs-25243/v1 ◽

2020 ◽

Author(s):

Maryam sadat Nezamtaheri ◽

Seyed Peyman Shariatpanahi ◽

Bahram Goliaei ◽

Alireza Madjid Ansari

Keyword(s):

Magnetic Field ◽

Cancer Cells ◽

Biological Effects ◽

Low Frequency ◽

Live Cells ◽

Magnetic Flux Density ◽

Dependent Manner ◽

Exposure System ◽

Wide Range ◽

Frequency Magnetic Field

Abstract Background Extremely low-frequency magnetic field (ELF-MF) significantly induces apoptosis in cancer cells. To study the biological effects of the ELF-MF on tumor cells, we have designed and constructed a new exposure system that provides a uniform magnetic field with negligible temperature fluctuations during the exposure. Additionally, it provides ideal physiological conditions for live cells inside the incubator. This ELF-MF exposure system eliminates several limitations and disadvantages of other low frequency magnetic field systems; it generates a magnetic field with a frequency of 0 to 70 Hz with a maximum magnetic flux density of 150 mT. Methods The capabilities of the setup were examined using a 1 Hz, 100 mT magnetic field, at various exposure times, to induce apoptosis-mediated cell death in the MC4-L2 cell line. After exposure, apoptosis was assessed by flow cytometry. Results A biphasic response was observed in cells exposed to ELF-MF: at first a decreasing apoptotic rate during 2-12 hours exposure time was detected, after which apoptosis gradually increased during 24-120 hours of exposure. Conclusions We show that ELF-MF exposure with a frequency of 1 Hz and intensity of 100 mT induces apoptosis in MC4-L2 cancer cells in a time-dependent manner. These results show the significance of the long term studies of the ELF exposure effects.

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59 Effect of Magnetic Field During Radiotherapy on Double-Strand DNA Breaks and Cell Proliferation on Prostate, Cervical, and Breast Cancer Cells

Radiotherapy and Oncology ◽

10.1016/s0167-8140(19)33347-x ◽

2019 ◽

Vol 139 ◽

pp. S27-S28

Author(s):

Deepak Dinakaran ◽

Sarah Baker ◽

Andrei Ghila ◽

Zhighang Jin ◽

Michael Hendzel ◽

...

Keyword(s):

Breast Cancer ◽

Magnetic Field ◽

Cell Proliferation ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Dna Breaks ◽

Double Strand Dna Breaks ◽

Double Strand Dna

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Abstract 727: Specific destruction of iron oxide labeled cancer cells by variable gradient magnetic field.

10.1158/1538-7445.am2013-727 ◽

2013 ◽

Author(s):

Sudath Hapuarachchige ◽

Yoshinori Kato ◽

Dmitri Artemov

Keyword(s):

Magnetic Field ◽

Iron Oxide ◽

Cancer Cells ◽

Gradient Magnetic Field

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A Static Magnetic Field Inhibits the Migration and Telomerase Function of Mouse Breast Cancer Cells

BioMed Research International ◽

10.1155/2020/7472618 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Zhu Fan ◽

Pingdong Hu ◽

Lekang Xiang ◽

Ying Liu ◽

Rongqiao He ◽

...

Keyword(s):

Breast Cancer ◽

Magnetic Field ◽

Cell Migration ◽

Cancer Cells ◽

Static Magnetic Field ◽

Breast Cancer Cells ◽

Mitotic Cell ◽

Therapeutic Modality ◽

Specific Marker ◽

Multiple Cancer

Static magnetic field (SMF) has a potential as a cancer therapeutic modality due to its specific inhibitory effects on the proliferation of multiple cancer cells. However, the underlying mechanism remains unclear, and just a few studies have examined the effects of SMF on metastasis, an important concern in cancer treatment. In this study, we evaluated the effects of moderate SMF (~150 mT) on the proliferation and migration of 4T1 breast cancer cells. Our results showed that SMF treatment accelerated cell proliferation but inhibited cell migration. Further, SMF treatment shortened the telomere length, decreased telomerase activity, and inhibited the expression of the cancer-specific marker telomerase reverse transcriptase (TERT), which may be related to expression upregulation of e2f1, a transcription repressor of TERT and positive regulator of the mitotic cell cycle. Our results revealed that SMF repressed both, cell migration and telomerase function. The telomerase network is responsive to SMF and may be involved in SMF-mediated cancer-specific effects; moreover, it may function as a therapeutic target in magnetic therapy of cancers.

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