Abstract 727: Specific destruction of iron oxide labeled cancer cells by variable gradient magnetic field.

2013 ◽  
Author(s):  
Sudath Hapuarachchige ◽  
Yoshinori Kato ◽  
Dmitri Artemov
Keyword(s):  
Magnetic Field ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Gradient Magnetic Field

scholarly journals Non-Temperature Induced Effects of Magnetized Iron Oxide Nanoparticles in Alternating Magnetic Field in Cancer Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0156294 ◽  
Author(s):  
Sudath Hapuarachchige ◽  
Yoshinori Kato ◽  
Ethel J. Ngen ◽  
Barbara Smith ◽  
Michael Delannoy ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Alternating Magnetic Field ◽  
Oxide Nanoparticles

scholarly journals A Novel Approach for Nucleic Acid Delivery Into Cancer Cells

Medicina ◽  
2012 ◽  
Vol 48 (6) ◽  
pp. 48 ◽  
Author(s):  
Dace Vainauska ◽  
Svetlana Kozireva ◽  
Andrejs Karpovs ◽  
Maksims Čistjakovs ◽  
Mihails Bariševs
Keyword(s):  
Magnetic Field ◽  
Cancer Cells ◽  
Cell Line ◽  
Incubation Time ◽  
Transfection Efficiency ◽  
Rotation Frequency ◽  
Superparamagnetic Nanoparticles ◽  
Gradient Magnetic Field ◽  
Delivery Methods ◽  
Dynamic Magnetic Field

Background. Liposomal magnetofection is based on the use of superparamagnetic particles and cationic lipids and shows better transfection efficiency than other common nonviral gene delivery methods; however, the distribution of aggregate complexes over the cell surface may be ununiform. The use of a dynamic gradient magnetic field could overcome this limitation. A newly developed device for magnetofection under a dynamic magnetic field was used to compare the transfection efficiency of prostate carcinoma cell line PC3 with that obtained by lipofection and magnetofection. Material and Methods. Reporter plasmid pcDNA3.1LacZ DNA was used in combination with Lipofectamine2000 reagent and superparamagnetic nanoparticles CombiMag. The effects of incubation time under a dynamic magnetic field and a rotation frequency of magnets on transfection efficiency for PC3 cell line were determined. Alternatively, lipofection and liposomal magnetofection were carried out. Transfection efficiency of delivery methods was estimated by β-galactosidase staining; cell viability, by acridine orange/ethidium bromide staining. Results. Liposomal magnetofection under a dynamic gradient magnetic field demonstrated the highest transfection efficiency: it was greater by almost 21% and 42% in comparison with liposomal magnetofection and lipofection, respectively. The optimal incubation time under dynamic magnetic field and the optimal magnet rotation frequency were 5 minutes and 5 rpm, respectively. Liposomal magnetofection under a dynamic gradient magnetic field was less cytotoxic (7%) than that under a permanent magnetic field (17%) and lipofection (11%). Conclusions. Our new approach, based on the use of a dynamic gradient magnetic field, enhanced the transfection efficiency and had a less cytotoxic effect on prostate cancer cells in comparison with the standard magnetofection and lipofection.

scholarly journals ID: 4001 Nanoparticle-based Cancer Therapy

2017 ◽  
Vol 4 (S) ◽  
pp. 2
Author(s):  
Fuyu Tamanoi
Keyword(s):  
Magnetic Field ◽  
Iron Oxide ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Tumor Targeting ◽  
Two Photon ◽  
Pore Opening ◽  
Oscillating Magnetic Field ◽  
The Way

Advances in Nanotechnology have led to the development of a variety of nanomaterials that are changing the way cancer therapy is carried out. A particularly important example is nanoparticle that can carry cargo to tumor. We are using mesoporous silica nanoparticles (MSNs) for cancer therapy. MSNs contain thousands of pores that provide storage space for anticancer drugs. These materials are biocompatible and safe. In addition, we have recently introduced biodegradability into MSNs.  We have shown that MSNs exhibit excellent tumor targeting capability in two different animal model systems (chicken egg tumor model and mouse xenografts). This tumor targeting capability is partly due to its small size; these nano-sized particles can accumulate in tumor due to leaky tumor vasculature. In addition, we have carried out surface modifications to attach ligands that bind receptors present on the surface of cancer cells. For example, folate was attached to the surface that enables binding to folate receptors overexpressed on cancer cells.  We have also conferred controlled anticancer drug release capability to MSNs in collaboration with Fraser Stoddart and Jeff Zink. This was accomplished by attaching nanovalves at the opening of the pores. Rotaxanes and pseudorotaxanes are used to prepare nanovalves. These chemical compounds consist of a stalk and a moving part. When the moving part is close to the pore opening, the nanovalve is closed. On the other hand, when the moving part is located away from the pore opening, the nanovalve is closed. In this way, the nanovalve provides an open and close function so that controlled release of anticancer drugs can be carried out.  Light activated nanovalves were developed by incorporating azobenzene into nanovalves. Azobenzene changes conformation upon light exposure and this conformational change opens the nanovalve releasing anticancer drugs in a power and exposure time dependent manner. More recently, this system was modified by incorporating two-photon dyes that can capture energy from two-photon light and transfer to azobenzene to drive the release of anticancer drugs. This enables the system to work with tissue penetrating two-photon light.  We have also developed nanoparticles that respond to oscillating magnetic field. This system was developed using MSNs that contain iron oxide core. Because of superparamagnetic property of iron oxide, the internal temperature of such nanoparticles increases when exposed to oscillating magnetic field. This temperature increase drives opening of nanovalves that are particularly designed for this purpose.   Development of nanoparticles that respond to external cues such as light and magnetic field may change the way cancer therapy is carried out. Implications on the future of cancer therapy will be discussed.

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

An Inductive Debris Sensor Based on a High-Gradient Magnetic Field

2019 ◽  
Vol 19 (8) ◽  
pp. 2879-2886 ◽  
Author(s):  
Song Feng ◽  
Leilei Yang ◽  
Guang Qiu ◽  
Jiufei Luo ◽  
Rui Li ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Gradient Magnetic Field ◽  
High Gradient

A 60-Hz sinusoidal magnetic field induces apoptosis of prostate cancer cells through reactive oxygen species

2008 ◽  
Vol 84 (11) ◽  
pp. 945-955 ◽  
Author(s):  
Eui Kwan Koh ◽  
Byung-Kyu Ryu ◽  
Dong-Young Jeong ◽  
Iel-Soo Bang ◽  
Myung Hee Nam ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Prostate Cancer ◽  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Prostate Cancer Cells ◽  
Sinusoidal Magnetic Field

Magnetic targeting with superparamagnetic iron oxide nanoparticles for in vivo glioma

2017 ◽  
Vol 6 (5) ◽  
pp. 449-472 ◽  
Author(s):  
Marina Fontes de Paula Aguiar ◽  
Javier Bustamante Mamani ◽  
Taylla Klei Felix ◽  
Rafael Ferreira dos Reis ◽  
Helio Rodrigues da Silva ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Process Efficiency ◽  
Cochrane Library ◽  
Oxide Nanoparticles ◽  
Magnetic Targeting

AbstractThe purpose of this study was to review the use of the magnetic targeting technique, characterized by magnetic driving compounds based on superparamagnetic iron oxide nanoparticles (SPIONs), as drug delivery for a specific brain locus in gliomas. We reviewed a process mediated by the application of an external static magnetic field for targeting SPIONs in gliomas. A search of PubMed, Cochrane Library, Scopus, and Web of Science databases identified 228 studies, 23 of which were selected based on inclusion criteria and predetermined exclusion criteria. The articles were analyzed by physicochemical characteristics of SPIONs used, cell types used for tumor induction, characteristics of experimental glioma models, magnetic targeting technical parameters, and analysis method of process efficiency. The study shows the highlights and importance of magnetic targeting to optimize the magnetic targeting process as a therapeutic strategy for gliomas. Regardless of the intensity of the patterned magnetic field, the time of application of the field, and nanoparticle used (commercial or synthesized), all studies showed a vast advantage in the use of magnetic targeting, either alone or in combination with other techniques, for optimized glioma therapy. Therefore, this review elucidates the preclinical and therapeutic applications of magnetic targeting in glioma, an innovative nanobiotechnological method.

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