Analysis of the upstream genetic structures of the IS Ecp1 ‐ bla CTX‐M transposition units in Escherichia coli isolates carrying bla CTX‐M obtained from the Indonesian and Vietnamese communities

2021 ◽  
Author(s):  
Fikri S. Widyatama ◽  
Nobuyoshi Yagi ◽  
Rosantia Sarassari ◽  
Toshiro Shirakawa ◽  
Danh Tuyen Le ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Structures

Characterization of novel ybjG and dacC variants in Escherichia coli

2013 ◽  
Vol 62 (11) ◽  
pp. 1728-1734 ◽  
Author(s):  
Dongguo Wang ◽  
Enping Hu ◽  
Jiayu Chen ◽  
Xiulin Tao ◽  
Katelyn Gutierrez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
The Novel ◽  
Conventional Pcr ◽  
E Coli ◽  
Novel Variants ◽  
Restriction Sites ◽  
Genetic Structures

A total of 69 strains of Escherichia coli from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, ybjG, dacC and mdfA, by gene mapping with conventional PCR detection. Conjugation experiments demonstrated that these genes existed in plasmids that conferred resistance. Novel ybjG and dacC variants were isolated from E. coli strains EC2163 and EC2347, which were obtained from the sputum of intensive care unit patients. Genetic mapping showed that the genes were located on 8200 kb plasmid regions flanked by EcoRI restriction sites. Three distinct genetic structures were identified among the 11 PCR-positive strains of E. coli, and two contained the novel ybjG and dacC variants. The putative amino acid differences in the ybjG and dacC gene variants were characterized. These results provide evidence for novel variants of ybjG and dacC, and suggest that multiple drug resistance in hospital strains of E. coli depends on the synergistic function of ybjG, dacC and mdfA within three distinct genetic structures in conjugative plasmids.

scholarly journals Evidence for Recent Acquisition and Successful Transmission ofblaCTX-M-15in Salmonella enterica in South Korea

2013 ◽  
Vol 57 (5) ◽  
pp. 2383-2387 ◽  
Author(s):  
Min-Su Kang ◽  
Yong-Kuk Kwon ◽  
Jae-Young Oh ◽  
Mi-Jin Kim ◽  
Douglas R. Call ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Salmonella Enterica ◽  
Content Type ◽  
Successful Transmission ◽  
Resistance Phenotype ◽  
E Coli ◽  
Genetic Structures

ABSTRACTWe identified two distinctblaCTX-M-bearing and five distinctblaCMY-2-bearing genetic structures located on plasmids fromSalmonella entericaandEscherichia coliisolates (n= 35) collected from chickens in South Korea. AllSalmonellaplasmids shared a common replicon,blaCTX-M-15transposon, and core resistance phenotype, whileE. coli blaCTX-M-15plasmids included four distinct replicons.

scholarly journals Genetic structures of the B2 and B1 Escherichia coli strains responsible for extra-intestinal infections

1993 ◽  
Vol 139 (12) ◽  
pp. 3079-3088 ◽  
Author(s):  
B. Picard ◽  
C. Journet-Mancy ◽  
N. Picard-Pasquier ◽  
P. Goullet
Keyword(s):  
Escherichia Coli ◽  
Intestinal Infections ◽  
Genetic Structures

scholarly journals Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

2021 ◽  
Vol 11 ◽  
Author(s):  
Hazem Ramadan ◽  
Ahmed M. Soliman ◽  
Lari M. Hiott ◽  
Mohammed Elbediwi ◽  
Tiffanie A. Woodley ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Core Genome ◽  
Multidrug Resistant ◽  
E Coli ◽  
Allelic Differences ◽  
Chicken Isolate ◽  
Genetic Structures ◽  
Retail Food ◽  
M 14

In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, blaCTX-M-14 was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The blaCTX-M-14 and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96–97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (≤200 core genome allelic differences). As E. coli co-harboring mcr-1 and fosA4 genes has only been recently reported, this study shows rapid spread of this genotype that shares similar genetic structures with regional and international E. coli lineages originating from both humans and food animals. Adopting WGS-based surveillance system is warranted to facilitate monitoring the international spread of MDR pathogens.

scholarly journals blaNDM-5-Bearing IncFII-Type Plasmids of Klebsiella pneumoniae Sequence Type 147 Transmitted by Cross-Border Transfer of a Patient

2016 ◽  
Vol 60 (3) ◽  
pp. 1932-1934 ◽  
Author(s):  
Juyoun Shin ◽  
Jin Yang Baek ◽  
Sun Young Cho ◽  
Hee Jae Huh ◽  
Nam Yong Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
South Korea ◽  
United Arab Emirates ◽  
Sequence Type ◽  
Content Type ◽  
Korean Patient ◽  
Cross Border ◽  
Genetic Structures

The two plasmids extracted fromKlebsiella pneumoniaesequence type 147 (ST147) isolates were analyzed. The first isolate was obtained from a patient transferred from United Arab Emirates to South Korea. The second isolate was obtained from a Korean patient and was suspected to be transmitted from the first patient. Sequences of two plasmids were almost the same, and genetic structures, includingblaNDM-5, of these plasmids were similar to plasmids of NDM-1-producingEscherichia coliST131 isolates found in Europe.

scholarly journals bla CTX-M Genes in Escherichia coli Strains from Croatian Hospitals Are Located in New (blaCTX-M-3a) and Widely Spread (blaCTX-M-3a and blaCTX-M-15) Genetic Structures

2009 ◽  
Vol 53 (4) ◽  
pp. 1630-1635 ◽  
Author(s):  
Elżbieta Literacka ◽  
Branka Bedenic ◽  
Anna Baraniak ◽  
Janusz Fiett ◽  
Marija Tonkic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clonal Group ◽  
E Coli ◽  
Kluyvera Ascorbata ◽  
Genetic Structures

ABSTRACT CTX-M-producing Escherichia coli isolates from three Croatian hospitals were analyzed. All bla CTX-M-15 genes and one bla CTX-M-3a gene resided in widely spread ISEcp1 transposition modules, but other bla CTX-M-3a genes were in a new configuration with two IS26 copies, indicating a new event of gene mobilization from a Kluyvera ascorbata genome. The study confirmed the role of the E. coli ST131 clonal group with IncFII-type plasmids in the spread of bla CTX-M-15 and of IncL/M pCTX-M3-type plasmids in the dissemination of bla CTX-M-3a.

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

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