scholarly journals blaNDM-5-Bearing IncFII-Type Plasmids of Klebsiella pneumoniae Sequence Type 147 Transmitted by Cross-Border Transfer of a Patient

2016 ◽  
Vol 60 (3) ◽  
pp. 1932-1934 ◽  
Author(s):  
Juyoun Shin ◽  
Jin Yang Baek ◽  
Sun Young Cho ◽  
Hee Jae Huh ◽  
Nam Yong Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
South Korea ◽  
United Arab Emirates ◽  
Sequence Type ◽  
Content Type ◽  
Korean Patient ◽  
Cross Border ◽  
Genetic Structures

The two plasmids extracted fromKlebsiella pneumoniaesequence type 147 (ST147) isolates were analyzed. The first isolate was obtained from a patient transferred from United Arab Emirates to South Korea. The second isolate was obtained from a Korean patient and was suspected to be transmitted from the first patient. Sequences of two plasmids were almost the same, and genetic structures, includingblaNDM-5, of these plasmids were similar to plasmids of NDM-1-producingEscherichia coliST131 isolates found in Europe.

scholarly journals Multihospital Occurrence of Pan-Resistant Klebsiella pneumoniae Sequence Type 147 with an ISEcp1-Directed blaOXA-181 Insertion in the mgrB Gene in the United Arab Emirates

2017 ◽  
Vol 61 (7) ◽  
Author(s):  
Ágnes Sonnevend ◽  
Akela Ghazawi ◽  
Rayhan Hashmey ◽  
Aliasgher Haidermota ◽  
Safinaz Girgis ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
South Korea ◽  
United Arab Emirates ◽  
Resistance Genes ◽  
Extended Period ◽  
Sequence Type ◽  
Resistant Clone ◽  
Colistin Resistance ◽  
Content Type ◽  
Resistance Island

ABSTRACT The emergence of pan-resistant Klebsiella pneumoniae strains is an increasing concern. In the present study, we describe a cluster of 9 pan-resistant K. pneumoniae sequence type 147 (ST147) isolates encountered in 4 patients over nearly 1 year in 3 hospitals of the United Arab Emirates (UAE). The isolates exhibited highly similar genotypes. All produced chromosomally encoded OXA-181, and the majority also produced the NDM-5 carbapenemase. As with the previously described single isolate from the UAE, MS6671, the mgrB was disrupted by a functional, ISEcp1-driven bla OXA-181 insertion causing resistance to carbapenems. The mutation was successfully complemented with an intact mgrB gene, indicating that it was responsible for colistin resistance. bla NDM-5 was located within a resistance island of an approximately 100-kb IncFII plasmid carrying ermB, mph(A), bla TEM-1B, rmtB, bla NDM-5, sul1, aadA2, and dfrA12 resistance genes. Sequencing this plasmid (pABC143-NDM) revealed that its backbone was nearly identical to that of plasmid pMS6671E from which several resistance genes, including bla NDM-5, had been deleted. More extensive similarities of the backbone and the resistance island were found between pABC143C-NDM and the bla NDM-5-carrying IncFII plasmids of two K. pneumoniae ST147 isolates from South Korea, one of which was colistin resistant, and both also produced OXA-181. Notably, one of these strains was isolated from a patient transferred from the UAE. Our data show that this pan-resistant clone has an alarming capacity to maintain itself over an extended period of time and is even likely to be transmitted internationally.

scholarly journals A Plasmid Bearing theblaCTX-M-15Gene and Phage P1-Like Sequences from a Sequence Type 11 Klebsiella pneumoniae Isolate

2015 ◽  
Vol 59 (10) ◽  
pp. 6608-6610 ◽  
Author(s):  
Juyoun Shin ◽  
Kwan Soo Ko
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Resistance Genes ◽  
Sequence Type ◽  
Content Type ◽  
Plasmid Backbone

ABSTRACTPlasmid pKP12226 was extracted and analyzed from a CTX-M-15-producingKlebsiella pneumoniaesequence type 11 (ST11) isolate collected in South Korea. The plasmid represents chimeric characteristics consisting of a pIP1206-like backbone and lysogenized phage P1-like sequences. It bears a resistance region that includes resistance genes to several antibiotics and is different from previously characterized plasmids from South Korea bearingblaCTX-M-15. It may have resulted from recombination between anEscherichia coliplasmid backbone, ablaCTX-M-15-bearing resistance region, and lysogenized phage P1-like sequences.

scholarly journals Evidence for Recent Acquisition and Successful Transmission ofblaCTX-M-15in Salmonella enterica in South Korea

2013 ◽  
Vol 57 (5) ◽  
pp. 2383-2387 ◽  
Author(s):  
Min-Su Kang ◽  
Yong-Kuk Kwon ◽  
Jae-Young Oh ◽  
Mi-Jin Kim ◽  
Douglas R. Call ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Salmonella Enterica ◽  
Content Type ◽  
Successful Transmission ◽  
Resistance Phenotype ◽  
E Coli ◽  
Genetic Structures

ABSTRACTWe identified two distinctblaCTX-M-bearing and five distinctblaCMY-2-bearing genetic structures located on plasmids fromSalmonella entericaandEscherichia coliisolates (n= 35) collected from chickens in South Korea. AllSalmonellaplasmids shared a common replicon,blaCTX-M-15transposon, and core resistance phenotype, whileE. coli blaCTX-M-15plasmids included four distinct replicons.

scholarly journals Complete Sequence of a Conjugative IncN Plasmid HarboringblaKPC-2,blaSHV-12, andqnrS1from an Escherichia coli Sequence Type 648 Strain

2014 ◽  
Vol 58 (11) ◽  
pp. 6974-6977 ◽  
Author(s):  
Jun-Jie Li ◽  
Chang-Seop Lee ◽  
Ji-Fang Sheng ◽  
Yohei Doi
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Clinical History ◽  
Plasmid Profile ◽  
Complete Sequence ◽  
Sequence Type ◽  
Fluoroquinolone Resistance ◽  
Content Type

ABSTRACTWe sequenced a novel conjugativeblaKPC-2-harboring IncN plasmid, pYD626E, from anEscherichia colisequence type 648 strain previously identified in Pittsburgh, Pennsylvania. pYD626E was 72,800 bp long and carried four β-lactamase genes,blaKPC-2,blaSHV-12,blaLAP-1, andblaTEM-1. In addition, it harboredqnrS1(fluoroquinolone resistance) anddfrA14(trimethoprim resistance). The plasmid profile and clinical history supported thein vivotransfer of this plasmid betweenKlebsiella pneumoniaeandEscherichia coli.

scholarly journals mcr-1.2, a NewmcrVariant Carried on a Transferable Plasmid from a Colistin-Resistant KPC Carbapenemase-Producing Klebsiella pneumoniae Strain of Sequence Type 512

2016 ◽  
Vol 60 (9) ◽  
pp. 5612-5615 ◽  
Author(s):  
Vincenzo Di Pilato ◽  
Fabio Arena ◽  
Carlo Tascini ◽  
Antonio Cannatelli ◽  
Lucia Henrici De Angelis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Klebsiella Pneumoniae ◽  
Rectal Swab ◽  
Sequence Type ◽  
Content Type ◽  
Functional Variant ◽  
Leukemic Child

ABSTRACTA novelmcrvariant, namedmcr-1.2, encoding a Gln3-to-Leu functional variant of MCR-1, was detected in a KPC-3-producing ST512Klebsiella pneumoniaeisolate collected in Italy from a surveillance rectal swab from a leukemic child. Themcr-1.2gene was carried on a transferable IncX4 plasmid whose structure was very similar to that ofmcr-1-bearing plasmids previously found inEscherichia coliandK. pneumoniaestrains from geographically distant sites (Estonia, China, and South Africa).

Emergence and spread of NDM-1 producer Enterobacteriaceae with contribution of IncX3 plasmids in the United Arab Emirates

2013 ◽  
Vol 62 (7) ◽  
pp. 1044-1050 ◽  
Author(s):  
Agnes Sonnevend ◽  
Amna Al Baloushi ◽  
Akela Ghazawi ◽  
Rayhan Hashmey ◽  
Safinaz Girgis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Middle East ◽  
Klebsiella Pneumoniae ◽  
United Arab Emirates ◽  
Indian Subcontinent ◽  
Enterobacter Cloacae ◽  
Sequence Type ◽  
Restriction Patterns ◽  
Foreign Travel ◽  
Similar Restriction

Among 28 clinically relevant, carbapenem non-susceptible Enterobacteriaceae isolates collected in 2009–2011 in the United Arab Emirates three Klebsiella pneumoniae, two Escherichia coli, one Enterobacter cloacae and one Citrobacter freundi were identified to produce NDM-1 carbapenemase. Unexpectedly, with the exception of a K. pneumoniae strain, sequence type ST11, originally acquired in India and subsequently spread nosocomially in the UAE, the majority of the strains could not be directly linked to foreign travel. All isolates harboured the bla NDM-1 gene on plasmids of IncA/C, IncHI1b and IncX3 types, or were untypable. IncX3 type plasmids with a mass of 50 kb and with the same or highly similar restriction patterns, with regions flanking the bla NDM-1 gene identical to the IncX3 NDM plasmids described from China were present in three different species, Enterobacter cloacae, Escherichia coli and C. freundii. Our findings strongly support the assumptions that, beyond the Indian subcontinent, the Middle East is an important reservoir of NDM-producing organisms. Furthermore, we also provide evidence that IncX3 plasmids, recently implicated in the spread of bla NDM-1 in China, have been widely distributed and are important vehicles of the inter-species spread of the NDM-1 gene.

scholarly journals Various Sequence Types of Enterobacteriaceae Isolated from Commercial Chicken Farms in China and Carrying the blaNDM-5 Gene

2018 ◽  
Vol 62 (10) ◽  
Author(s):  
Rong Xiang ◽  
An-Yun Zhang ◽  
Xiao-Lan Ye ◽  
Zhuang-Zhuang Kang ◽  
Chang-Wei Lei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Content Type ◽  
Commercial Chicken ◽  
Rectal Swabs ◽  
Sequence Types

ABSTRACT A total of 108 meropenem-resistant Enterobacteriaceae isolates were obtained from 1,658 rectal swabs collected from 15 unrelated commercial chicken farms in China between 2014 and 2016. These samples yielded 16 Escherichia coli and 2 Klebsiella pneumoniae isolates of diverse sequence types carrying a blaNDM-5-bearing IncX3 plasmid. K. pneumoniae strain sequence type 709 (ST709) has two blaNDM-5-carrying plasmids that were transferred together to E.coli.

scholarly journals Complete Sequences ofmcr-1-Harboring Plasmids from Extended-Spectrum-β-Lactamase- and Carbapenemase-Producing Enterobacteriaceae

2016 ◽  
Vol 60 (7) ◽  
pp. 4351-4354 ◽  
Author(s):  
Aiqing Li ◽  
Yong Yang ◽  
Minhui Miao ◽  
Kalyan D. Chavda ◽  
José R. Mediavilla ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Incompatibility Group ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Complete Sequences ◽  
Group A

ABSTRACTHere we completely sequenced fourmcr-1-haboring plasmids, isolated from two extended-spectrum-β-lactamase (ESBL)-producingEscherichia coliand two carbapenemase-producingKlebsiella pneumoniaeclinical isolates. Themcr-1-harboring plasmids from anE. colisequence type 2448 (ST2448) isolate and twoK. pneumoniaeST25 isolates were identical (all pMCR1-IncX4), belonging to the IncX4 incompatibility group, while the plasmid from anE. coliST2085 isolate (pMCR1-IncI2) belongs to the IncI2 group. A nearly identical 2.6-kbmcr-1-pap2element was found to be shared by allmcr-1-carrying plasmids.

scholarly journals Characteristics of Escherichia coli Sequence Type 131 Isolates That Produce Extended-Spectrum β-Lactamases: Global Distribution of theH30-Rx Sublineage

2014 ◽  
Vol 58 (7) ◽  
pp. 3762-3767 ◽  
Author(s):  
Gisele Peirano ◽  
Akke K. van der Bij ◽  
Joshua L. Freeman ◽  
Laurent Poirel ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
United Arab Emirates ◽  
The United States ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Global Distribution ◽  
Phylogenetic Groups ◽  
Content Type ◽  
Specific Pcr ◽  
Extended Spectrum

ABSTRACTWe designed a study to describe the characteristics of sequence type 131 (ST131) lineages, including theH30-Rx sublineage, among a global collection of extended-spectrum β-lactamase (ESBL)-producingEscherichia coliisolates from 9 countries collected from 2000 to 2011. A total of 240 nonrepeat isolates from Canada, the United States, Brazil, the Netherlands, France, the United Arab Emirates (UAE), India, South Africa, and New Zealand were included. Established PCR, sequencing, and typing methods were used to define ST131 lineages,H30 andH30-Rx phylogenetic groups,gyrAandparCmutations, virotypes, and plasmid-mediated quinolone resistance determinants. The majority of the isolates produced CTX-M-15 withaac(6′)-lb-cr, belonged to phylogenetic group B2, and were positive for theH30 lineage with thegyrA1ABandparC1aABmutations. ST131 showed 15 distinct pulsotypes; 43% of the isolates belonged to four pulsotypes, with a global distribution. Seventy-five percent of the ST131 isolates belonged toH30-Rx; this sublineage was present in all the countries and was associated with multidrug resistance,blaCTX-M-15,aac(6′)-lb-cr, and virotypes A and C. TheH41 lineage was negative for the ST131pabBallele-specific PCR. The multidrug-resistantH30-Rx sublineage poses an important public health threat due to its global distribution, association with virotype C, and high prevalence among ST131 isolates that produce CTX-M-15.

A multi-institutional outbreak of New Delhi metallo-β-lactamase–producing Escherichia coli with subsequent acquisition of the Klebsiella pneumoniae carbapenemase gene

2020 ◽  
pp. 1-4
Author(s):  
Ester Solter ◽  
Jason C. Kwong ◽  
Aaron Walton ◽  
Norelle Sherry ◽  
Benjamin P. Howden ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Common Ancestor ◽  
Sequence Type ◽  
Second Phase ◽  
New Delhi ◽  
Genetic Sequencing ◽  
Klebsiella Pneumoniae Carbapenemase ◽  
Carbapenemase Gene

Abstract We characterized 57 isolates from a 2-phase clonal outbreak of New Delhi metallo-β-lactamase–producing Eschericha coli, involving 9 Israeli hospitals; all but 1 isolate belonged to sequence-type (ST) 410. Most isolates in the second phase harbored blaKPC-2 in addition to blaNDM-5. Genetic sequencing revealed most dual-carbapenemase–producing isolates to be monophyletically derived from a common ancestor.

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