scholarly journals Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources in Microcystis aeruginosa NIES ‐843

2020 ◽  
Vol 22 (6) ◽  
pp. 2419-2431
Author(s):  
Lauren E. Krausfeldt ◽  
Abigail T. Farmer ◽  
Hector F. Castro ◽  
Gregory L. Boyer ◽  
Shawn R. Campagna ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Nitrogen Sources ◽  
Nitrogen Flux

EFFECTS OF pH AND NITROGEN SOURCES ON GROWTH OF MICROCYSTIS AERUGINOSA KÜTZ.

1962 ◽  
Vol 8 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Jack McLachlan ◽  
P. R. Gorham
Keyword(s):  
Growth Inhibition ◽  
Microcystis Aeruginosa ◽  
Nitrate Nitrogen ◽  
Nitrogen Sources ◽  
Cesium Chloride ◽  
Potassium Nitrate ◽  
Degree Of Ionization ◽  
Ph Values ◽  
Effects Of Ph ◽  
Ph Range

Microcystis aeruginosa Kütz. (strain NRC-1) grew equally well throughout the pH range 6.5 to 10 when provided with suitable media. Toxicity of tris(hydroxymethyl)aminomethane (TRIS) towards the alga was found to decrease as the pH decreased and could be correlated with the degree of ionization of the TRIS molecule. Other organic buffers examined were either toxic at all concentrations and pH values tested or promoted lysis. When TRIS was used as a buffer, higher concentrations of cesium chloride and potassium nitrate were tolerated without growth inhibition at pH 6.5 than at 7.5. In the presence of TRIS, Microcystis grew equally well with nitrate, ammonium, or urea as nitrogen sources. Eight out of 20 amino compounds examined served as nitrogen sources in TRIS-buffered medium, but growth was poorer than with nitrate nitrogen.

The Correlation between Nitrogen Phosphorus Nutrients and the Growth of Microcystis Aeruginosa

2014 ◽  
Vol 1065-1069 ◽  
pp. 3110-3113
Author(s):  
Jun Sen Wu ◽  
Chen Chen Liu ◽  
Rui Bao Jia ◽  
Bing Li
Keyword(s):  
Nitrogen Source ◽  
Microcystis Aeruginosa ◽  
Nitrogen Sources ◽  
Limiting Factor ◽  
Nitrogen And Phosphorus ◽  
Optimal Ratio ◽  
Water Eutrophication ◽  
Nitrogen Phosphorus

The correction of nitrogen phosphorus nutrients and the Microcystis aeruginosa biomass were studied by setting the different concentration of nitrogen and phosphorus, different nitrogen sources and the ratios of nitrogen and phosphorus. The results shown that the optimal nitrogen source on the growth of Microcytis aeruginosa NaNO3, Miccrocystis aeruginosa could adapt to a low N environment. Phosphorus is the limiting factor in the growth of Microcystis aeruginosa that can not survive at the absence of P in the environment. The optimal ratio of nitrogen to phosphorus is 50:1. Glycine as the Microcystis aeruginosa nutrient grew slowly at prior period and it grew rapidly later, which is similarity to the water eutrophication and the algae outbreak.

Algicidal activity of an actinomycete strain, Streptomyces rameus, against Microcystis aeruginosa

2016 ◽  
Vol 74 (6) ◽  
pp. 1398-1408 ◽  
Author(s):  
Kanchariya Phankhajon ◽  
Anchana Somdee ◽  
Theerasak Somdee
Keyword(s):  
Microcystis Aeruginosa ◽  
Continuous Flow ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Statistical Technique ◽  
Culture Conditions ◽  
Algicidal Activity ◽  
Initial Ph ◽  
Actinomycete Strain ◽  
Airlift Loop

An actinomycete strain (KKU-A3) with algicidal activity against Microcystis aeruginosa was isolated from soil in Khon Kaen Province, Thailand. Based on its phenotypic characteristics and 16S rDNA sequence, strain KKU-A3 was identified as Streptomyces rameus. Strain KKU-A3 also exhibited algicidal activity against the cyanobacteria Synechococcus elongatus, Cylindrospermum sp. and Oscillatoria sp. A mathematical and statistical technique was used to optimize the culture conditions and maximize its anti-Microcystis activity. The single factor experiments indicated that glucose and casein were the most effective carbon and nitrogen sources, respectively, and produced the highest anti-Microcystis activity. Response surface methodology indicated that the optimum culture conditions were 19.81 g/L glucose and 2.0 g/L casein at an initial pH of 7.8 and an incubation temperature of 30 °C. The anti-Microcystis activity increased from 82% to 95% under optimum conditions. In an internal airlift loop bioreactor, the removal of M. aeruginosa KKU-13 by the bacterium was investigated in batch and continuous flow experiments. In the batch experiment, KKU-A3 displayed maximum anti-Microcystis activity of 95% at day 7, whereas in the continuous flow experiment, KKU-A3 displayed maximum anti-Microcystis activity of 95% at day 10.

Influence of increased nutrient supply on Microcystis aeruginosa at cellular and proteomic levels

2020 ◽  
Vol 85 ◽  
pp. 47-58
Author(s):  
Y Jiang ◽  
Y Liu
Keyword(s):  
Microcystis Aeruginosa ◽  
Regulatory Protein ◽  
Acid Synthesis ◽  
Nutrient Supply ◽  
Nitrogen Supply ◽  
Nitrogen And Phosphorus ◽  
Comprehensive Description ◽  
Amino Acid Synthesis ◽  
High Nutrient ◽  
Proteomic Responses

Various studies have observed that increased nutrient supply promotes the growth of bloom-forming cyanobacteria, but only a limited number of studies have investigated the influence of increased nutrient supply on bloom-forming cyanobacteria at the proteomic level. We investigated the cellular and proteomic responses of Microcystis aeruginosa to elevated nitrogen and phosphorus supply. Increased supply of both nutrients significantly promoted the growth of M. aeruginosa and the synthesis of chlorophyll a, protein, and microcystins. The release of microcystins and the synthesis of polysaccharides negatively correlated with the growth of M. aeruginosa under high nutrient levels. Overexpressed proteins related to photosynthesis, and amino acid synthesis, were responsible for the stimulatory effects of increased nutrient supply in M. aeruginosa. Increased nitrogen supply directly promoted cyanobacterial growth by inducing the overexpression of the cell division regulatory protein FtsZ. NtcA, that regulates gene transcription related to both nitrogen assimilation and microcystin synthesis, was overexpressed under the high nitrogen condition, which consequently induced overexpression of 2 microcystin synthetases (McyC and McyF) and promoted microcystin synthesis. Elevated nitrogen supply induced the overexpression of proteins involved in gas vesicle organization (GvpC and GvpW), which may increase the buoyancy of M. aeruginosa. Increased phosphorus level indirectly affected growth and the synthesis of cellular substances in M. aeruginosa through the mediation of differentially expressed proteins related to carbon and phosphorus metabolism. This study provides a comprehensive description of changes in the proteome of M. aeruginosa in response to an increased supply of 2 key nutrients.

Linking nitrogen sources utilised by seagrass in a temperate marine embayment to patterns of seagrass change during drought

2016 ◽  
Vol 549 ◽  
pp. 79-88 ◽  
Author(s):  
AJ Hirst ◽  
AR Longmore ◽  
D Ball ◽  
PLM Cook ◽  
GP Jenkins
Keyword(s):  
Nitrogen Sources ◽  
Marine Embayment

Effect of Nitrogen Sources on the AZO Dye Decolourization

2012 ◽  
Vol 2 (7) ◽  
pp. 424-426
Author(s):  
Suchita Dahiwade ◽  
◽  
Dr. A. O. Ingle Dr. A. O. Ingle ◽  
Dr. S. R. Wate Dr. S. R. Wate
Keyword(s):  
Azo Dye ◽  
Nitrogen Sources ◽  
Dye Decolourization

IDENTIFICATION AND CHARACTERIZATION OF A PURPLE NONSULFUR BACTERIUM ISOLATED FROM COASTAL AREA OF HAI PHONG FOR USING IN PRODUCTION OF UNSATURATED FATTY ACID (OMEGA 6, 7, 9)

2019 ◽  
Vol 57 (6) ◽  
pp. 665 ◽  
Author(s):  
Yen Thi Hoang ◽  
Quynh Thi Thu Tran ◽  
Ha Hoang Chu ◽  
Tuyen Thi Do ◽  
Thanh Tat Dang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Nitrogen Sources ◽  
16S Rrna Analysis ◽  
Rhodovulum Sulfidophilum ◽  
Purple Nonsulfur Bacterium ◽  
High Lipid ◽  
Physiological Properties ◽  
Omega 6

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.

Sarcosine and Creatine as Nitrogen Sources for the Ruminant, and the Influence of Supplemental Vitamin B12

1959 ◽  
Vol 18 (2) ◽  
pp. 780-789 ◽  
Author(s):  
C. D. Campbell ◽  
G. A. McLaren ◽  
G. C. Anderson ◽  
J. A. Welch ◽  
G. S. Smith ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Nitrogen Sources ◽  
Supplemental Vitamin

Inhibitory effects of Cyperus alternifolius on growth of Microcystis aeruginosa and identification of algicidal substances

2019 ◽  
Vol 46 (1) ◽  
pp. 73-84
Author(s):  
L. Zhou ◽  
S. Nakai ◽  
G. F. Chen ◽  
Q. Pan ◽  
N. X. Cui ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Inhibitory Effects ◽  
Cyperus Alternifolius
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