Whey protein hydrolysis with microbial proteases: Determination of kinetic parameters and bioactive properties for different reaction conditions

AbstractThe review, based on 92 references, is focused on degradation of organics by ozonation and it comprises various classes of oxygen-containing organic compounds - alcohols, ketones, ethers and hydroxybenzenes. The mechanisms of a multitude of ozone reactions with these compounds in organic solvents are discussed in details, presenting the respective reaction schemes and the corresponding kinetic parameters are given and some thermodynamic parameters are also listed. The dependencies of the kinetics and the mechanism of the ozonation reactions on the structure of the compounds, on the medium and on the reaction conditions are revealed. The various possible applications of ozonolysis are specified and discussed. All these reactions have practical importance for the protection of the environment.

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MEASUREMENT OF STEROID BINDING PROTEINS

Acta Endocrinologica ◽

10.1530/acta.0.065s104 ◽

1970 ◽

Vol 65 (1_Suppl) ◽

pp. S104-S121 ◽

Cited By ~ 24

Author(s):

E. E. Baulieu ◽

J. P. Raynaud ◽

E. Milgrom

Keyword(s):

Kinetic Parameters ◽

Binding Proteins ◽

Binding Specificity ◽

Binding Parameters ◽

Target Organs ◽

Biological Mixtures ◽

Steroid Binding Proteins ◽

Steroid Binding ◽

Steroid Binding Protein

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.

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Regeneration Mechanism Studied by Potential-Time Response to Sinusoidal Current-Time Function

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19971511 ◽

1997 ◽

Vol 62 (10) ◽

pp. 1511-1526

Author(s):

María-Luisa Alcaraz ◽

Ángela Molina

Keyword(s):

Kinetic Parameters ◽

Rate Constant ◽

Theoretical Study ◽

Time Function ◽

Time Response ◽

Current Time ◽

Regeneration Mechanism ◽

Sinusoidal Current ◽

Regeneration Processes

A theoretical study of the potential-time response to sinusoidal current applied to static and dynamic electrodes for regeneration processes is presented. Methods for determination of the regeneration fraction, rate constant of the chemical reaction and heterogeneous kinetic parameters are proposed.

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Software-guided microscale flow calorimeter for efficient acquisition of thermokinetic data

Journal of Flow Chemistry ◽

10.1007/s41981-021-00145-6 ◽

2021 ◽

Author(s):

Timothy Aljoscha Frede ◽

Marlene Dietz ◽

Norbert Kockmann

Keyword(s):

Design Of Experiments ◽

Process Development ◽

Reaction Conditions ◽

Experimental Conditions ◽

Flow Calorimeter ◽

Microscale Flow ◽

Increased Sensitivity ◽

Important Time ◽

Optimal Reaction

AbstractFast chemical process development is inevitably linked to an optimized determination of thermokinetic data of chemical reactions. A miniaturized flow calorimeter enables increased sensitivity when examining small amounts of reactants in a short time compared to traditional batch equipment. Therefore, a methodology to determine optimal reaction conditions for calorimetric measurement experiments was developed and is presented in this contribution. Within the methodology, short-cut calculations are supplemented by computational fluid dynamics (CFD) simulations for a better representation of the hydrodynamics within the microreactor. This approach leads to the effective design of experiments. Unfavourable experimental conditions for kinetics experiments are determined in advance and therefore, need not to be considered during design of experiments. The methodology is tested for an instantaneous acid-base reaction. Good agreement of simulations was obtained with experimental data. Thus, the prediction of the hydrodynamics is enabled and the first steps towards a digital twin of the calorimeter are performed. The flow rates proposed by the methodology are tested for the determination of reaction enthalpy and showed that reasonable experimental settings resulted. Graphical abstract A methodology is suggested to evaluate optimal reaction conditions for efficientacquisition of kinetic data. The experimental design space is limited by thestepwise determination of important time scales based on specified input data.

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