The Metabolism of Testosterone by Dermal Papilla Cells Cultured From Human Pubic and Axillary Hair Follicles Concurs with Hair Growth in 5α-Reductase Deficiency

Rab27a/b are known to play an important role in the transport of melanosomes, with their knockout causing silvery gray hair. However, the relationship between Rab27a/b and hair growth is not well known. To evaluate the role of Rab27a/b in hair cycle, we investigated the expression of Rab27a/b during hair cycling and human outer root sheath (hORS) cells. The expression of Rab27a in ORS cells was mainly detected at the anagen, whereas expression of Rab27b in ORS, and epidermal cells was strongly expressed at the telogen. Additionally, Rab27a/b were expressed in the Golgi of hORS cells. To evaluate the role of Rab27a/b in hair growth, telogen-to-anagen transition animal and vibrissae hair follicles (HFs) organ culture models were assayed using Rab27a/b siRNAs. The knockdown of Rab27a or Rab27b suppressed or promoted hair growth, respectively. These results were also confirmed in human dermal papilla cells (hDPCs) and hORS cells, showing the opposite mitogenic effects. Moreover, Rab27b knockdown increased the expression levels of various growth factors in the hDPCs and hORS cells. Overall, the opposite temporal expression patterns during hair cycling and roles for hair growth of Rab27a/b suggested that Rab27a/b might regulate the hair cycle. Therefore, our study may provide a novel solution for the development of hair loss treatment by regulating Rab27a/b levels.

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Preclinical and Clinical Studies Demonstrate That the Proprietary Herbal Extract DA-5512 Effectively Stimulates Hair Growth and Promotes Hair Health

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/4395638 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Jae Young Yu ◽

Biki Gupta ◽

Hyoung Geun Park ◽

Miwon Son ◽

Joon-Ho Jun ◽

...

Keyword(s):

Clinical Study ◽

Hair Growth ◽

Dermal Papilla ◽

Hair Follicles ◽

Herbal Extract ◽

Control Group ◽

Dependent Manner ◽

Ki 67 ◽

Dermal Papilla Cells

The proprietary DA-5512 formulation comprises six herbal extracts from traditional oriental plants historically associated with therapeutic and other applications related to hair. Here, we investigated the effects of DA-5512 on the proliferation of human dermal papilla cells (hDPCs) in vitro and on hair growth in C57BL/6 mice and conducted a clinical study to evaluate the efficacy and safety of DA-5512. DA-5512 significantly enhanced the viability of hDPCs in a dose-dependent manner (p<0.05), and 100 ppm of DA-5512 and 1 μM minoxidil (MXD) significantly increased the number of Ki-67-positive cells, compared with the control group (p<0.05). MXD (3%) and DA-5512 (1%, 5%) significantly stimulated hair growth and increased the number and length of hair follicles (HFs) versus the controls (each p<0.05). The groups treated with DA-5512 exhibited hair growth comparable to that induced by MXD. In clinical study, we detected a statistically significant increase in the efficacy of DA-5512 after 16 weeks compared with the groups treated with placebo or 3% MXD (p<0.05). In conclusion, DA-5512 might promote hair growth and enhance hair health and can therefore be considered an effective option for treating hair loss.

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Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles

Journal of Endocrinology ◽

10.1677/joe-07-0522 ◽

2008 ◽

Vol 197 (1) ◽

pp. 11-23 ◽

Cited By ~ 29

Author(s):

Valerie A Randall ◽

Tracey J Jenner ◽

Nigel A Hibberts ◽

Isabel O De Oliveira ◽

Tayyebeh Vafaee

Keyword(s):

Stem Cell ◽

Stem Cell Factor ◽

Hair Growth ◽

Dermal Papilla ◽

Hair Follicles ◽

Androgenetic Alopecia ◽

Hair Colour ◽

Outer Root Sheath ◽

Dermal Papilla Cells ◽

Factor C

Androgens stimulate many hair follicles to alter hair colour and size via the hair growth cycle; in androgenetic alopecia tiny, pale hairs gradually replace large, pigmented ones. Since stem cell factor (SCF) is important in embryonic melanocyte migration and maintaining adult rodent pigmentation, we investigated SCF/c-Kit signalling in human hair follicles to determine whether this was altered in androgenetic alopecia. Quantitative immunohistochemistry detected three melanocyte-lineage markers and c-Kit in four focus areas: the epidermis, infundibulum, hair bulb (where pigment is formed) and mid-follicle outer root sheath (ORS). Colocalisation confirmed melanocyte c-Kit expression; cultured follicular melanocytes also exhibited c-Kit. Few ORS cells expressed differentiated melanocyte markers or c-Kit, but NKI/beteb antibody, which also recognises early melanocyte-lineage antigens, identified fourfold more cells, confirmed by colocalisation. Occasional similar bulbar cells were seen. Melanocyte distribution, concentration and c-Kit expression were unaltered in balding follicles. Androgenetic alopecia cultured dermal papilla cells secreted less SCF, measured by ELISA, than normal cells. This identifies three types of melanocyte-lineage cells in human follicles. The c-Kit expression by dendritic, pigmenting, bulbar melanocytes and rounded, differentiated, non-pigmenting ORS melanocytes implicate SCF in maintaining pigmentation and migration into regenerating hair bulbs. Less differentiated, c-Kit-independent cells in the mid-follicle ORS stem cell niche and occasionally in the bulb, presumably a local reserve for long scalp hair growth, implicate other factors in activating stem cells. Androgens appear to reduce alopecia hair colour by inhibiting dermal papilla SCF production, impeding bulbar melanocyte pigmentation. These results may facilitate new treatments for hair colour changes in hirsutism, alopecia or greying.

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Kojyl Cinnamate Ester Derivatives Increase Adiponectin Expression and Stimulate Adiponectin-Induced Hair Growth Factors in Human Dermal Papilla Cells

International Journal of Molecular Sciences ◽

10.3390/ijms20081859 ◽

2019 ◽

Vol 20 (8) ◽

pp. 1859 ◽

Cited By ~ 6

Author(s):

Phil June Park ◽

Eun-Gyung Cho

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Mrna Expression ◽

Hair Growth ◽

Subcutaneous Fat ◽

Dermal Papilla ◽

Hair Follicles ◽

Dermal Papilla Cells ◽

Glucose Levels ◽

Endothelial Growth Factor

Adiponectin (APN), released mainly from adipose tissue, is a well-known homeostatic factor for regulating glucose levels, lipid metabolism, and insulin sensitivity. A recent study showed that human hair follicles express APN receptors and the presence of APN-mediated hair growth signaling, thereby suggesting that APN is a potent hair growth-promoting adipokine. Previously, kojyl cinnamate ester derivatives (KCEDs) were synthesized in our institute as new anti-aging or adiponectin-/adipogenesis-inducing compounds. Here, we tested the activity of these derivatives to induce endogenous APN secretion. Among the derivatives, KCED-1 and KCED-2 showed improved activity in inducing APN mRNA expression, secretion of APN protein, and adipogenesis in human subcutaneous fat cells (hSCFs) when compared with the effects of Seletinoid G, a verified APN inducer. When human follicular dermal papilla cells were treated with the culture supernatant of KCED-1- or KCED-2-treated hSCFs, the mRNA expression of APN-induced hair growth factors such as insulin-like growth factor, hepatocyte growth factor, and vascular endothelial growth factor was upregulated compared with that in the control. Taken together, our study shows that among kojyl cinnamate ester derivatives, KCED-1, KCED-2, as well as Seletinoid G are effective inducers of endogenous APN production in subcutaneous fat tissues, which may in turn contribute to the promotion of hair growth in the human scalp.

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Exosomes derived from human dermal papilla cells promote hair growth in cultured human hair follicles and augment the hair‐inductive capacity of cultured dermal papilla spheres

Experimental Dermatology ◽

10.1111/exd.13927 ◽

2019 ◽

Vol 28 (7) ◽

pp. 854-857 ◽

Cited By ~ 12

Author(s):

Mi H. Kwack ◽

Chang H. Seo ◽

Prakash Gangadaran ◽

Byeong‐Cheol Ahn ◽

Moon K. Kim ◽

...

Keyword(s):

Human Hair ◽

Hair Growth ◽

Dermal Papilla ◽

Hair Follicles ◽

Dermal Papilla Cells

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Molecular Mechanisms of Hair Growth and Regeneration: Current Understanding and Novel Paradigms

Dermatology ◽

10.1159/000506155 ◽

2020 ◽

Vol 236 (4) ◽

pp. 271-280 ◽

Cited By ~ 7

Author(s):

Khosrow Siamak Houschyar ◽

Mimi R. Borrelli ◽

Christian Tapking ◽

Daniel Popp ◽

Behrus Puladi ◽

...

Keyword(s):

Hair Growth ◽

Molecular Mechanisms ◽

Dermal Papilla ◽

Hair Follicles ◽

Current Understanding ◽

Special Focus ◽

Cellular Interactions ◽

Molecular Signaling ◽

Dermal Papilla Cells ◽

Stem And Progenitor Cells

Hair is a defining feature of mammals and has critical functions, including protection, production of sebum, apocrine sweat and pheromones, social and sexual interactions, thermoregulation, and provision of stem cells for skin homeostasis, regeneration, and repair. The hair follicle (HF) is considered a “mini-organ,” consisting of intricate and well-organized structures which originate from HF stem and progenitor cells. Dermal papilla cells are the main components of the mesenchymal compartments in the hair bulb and are instrumental in generating signals to regulate the behavior of neighboring epithelial cells during the hair cycle. Mesenchymal-epithelial interactions within the dermal papilla niche drive HF embryonic development as well as the postnatal hair growth and regeneration cycle. This review summarizes the current understanding of HF development, repair, and regeneration, with special focus on cell signaling pathways governing these processes. In particular, we discuss emerging paradigms of molecular signaling governing the dermal papilla-epithelial cellular interactions during hair growth and maintenance and the recent progress made towards tissue engineering of human hair follicles.

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Morus alba Root Extract Induces the Anagen Phase in the Human Hair Follicle Dermal Papilla Cells

Pharmaceutics ◽

10.3390/pharmaceutics13081155 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1155

Author(s):

Jiyu Hyun ◽

Jisoo Im ◽

Sung-Won Kim ◽

Han Young Kim ◽

Inwoo Seo ◽

...

Keyword(s):

Hair Follicle ◽

Hair Growth ◽

Dermal Papilla ◽

Hair Follicles ◽

Morus Alba ◽

Root Extract ◽

Dermal Papilla Cells ◽

Anagen Phase ◽

Factor Secretion ◽

Growth Factor Secretion

Restoring hair follicles by inducing the anagen phase is a promising approach to prevent hair loss. Hair follicle dermal papilla cells (HFDPCs) play a major role in hair growth via the telogen-to-anagen transition. The therapeutic effect of Morus alba activates β-catenin in HFDPCs, thereby inducing the anagen phase. The HFDPCs were treated with M. alba root extract (MARE) to promote hair growth. It contains chlorogenic acid and umbelliferone and is not cytotoxic to HFDPCs at a concentration of 20%. It was demonstrated that a small amount of MARE enhances growth factor secretion (related to the telogen-to-anagen transition). Activation of β-catenin was observed in MARE-treated HFDPCs, which is crucial for inducing the anagen phase. The effect of conditioned medium derived from MARE-treated HFDPCs on keratinocytes and endothelial cells was also investigated. The findings of this study demonstrate the potency of MARE in eliciting the telogen-to-anagen transition.

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