Acute or Chronic Topical Retinoic Acid Treatment of Human Skin In Vivo Alters the Expression of Epidermal Transglutaminase, Loricrin, Involucrin, Filaggrin, and Keratins 6 and 13 but not Keratins 1, 10, and 14

Chondrogenesis in the fetal hamster tibia was examined 24, 36 and 48 h after maternaladministration of retinoic acid at a known teratogenic dose (80 mg/kg) on day 10 of gestation. Twenty-four hours after treatment the tibial chondroblasts were more closely packed with less intervening fibrillar or granular matrix than in the controls. By 36 h posttreatment, an intercellular matrix containing wide clumped fibrils unassociated with matrix granules had begun to accumulate, while the chondroblasts had a relatively poorly developed Golgi apparatus and smooth, non-scalloped cell outlines. Cells frequently maintained contact through pseudopodial processes. Forty-eight hours after retinoic acid treatment, the chondroblasts appeared similar to controls of the same age. It is suggested that the paucity and abnormality of the extracellular matrix prevents the chondroblasts from assuming their normal spatial relations with each other, resulting in a small skeletal blastema.

Download Full-text

Vitamin A Supplementation and Retinoic Acid Treatment in the Regulation of Antibody Responses In Vivo

Vitamin A - Vitamins & Hormones ◽

10.1016/s0083-6729(06)75008-7 ◽

2007 ◽

pp. 197-222 ◽

Cited By ~ 38

Author(s):

A. Catharine Ross

Keyword(s):

Retinoic Acid ◽

Vitamin A ◽

Acid Treatment ◽

Antibody Responses ◽

Retinoic Acid Treatment ◽

Vitamin A Supplementation

Download Full-text

In Vivo Effects of 13-cis Retinoic Acid Treatment on the Concentration of Proteins and Lipids in Serum

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.1996.34.1.3 ◽

1996 ◽

Vol 34 (1) ◽

Author(s):

Göran A. Fex ◽

Annika Aronsson ◽

Anders Andersson ◽

Kerstin Larsson ◽

Peter Nilsson-Ehle

Keyword(s):

Retinoic Acid ◽

Acid Treatment ◽

Retinoic Acid Treatment ◽

In Vivo Effects

Download Full-text

In Vivo Modulation of Connexins 43 and 26 of Human Epidermis by Topical Retinoic Acid Treatment

Journal of Histochemistry & Cytochemistry ◽

10.1177/002215549704500904 ◽

1997 ◽

Vol 45 (9) ◽

pp. 1207-1215 ◽

Cited By ~ 43

Author(s):

Elisabeth Masgrau–Peya ◽

Denis Salomon ◽

Jean-Hilaire Saurat ◽

Paolo Meda

Keyword(s):

Retinoic Acid ◽

Acid Treatment ◽

Fold Increase ◽

Northern Blotting ◽

Hair Follicles ◽

Human Epidermis ◽

Sweat Glands ◽

Epidermal Keratinocytes ◽

Retinoic Acid Treatment

After 14 weeks of topical application of 0.1% all- trans-retinoic acid to the napes of volunteers, we observed a 2.5-fold increase in the thickness of epidermis, owing to an increase ( p <0.001) in the number and size of keratinocytes and the induction of keratin 6. These changes in the differentiation of epidermal keratinocytes were paralleled by an increase in the amount of Cx43, a connexin that is normally expressed in human epidermis, and by the massive induction of Cx26, which is barely detectable in normal interfollicular epidermis, as judged at both the transcript (Northern blotting) and the protein level (immunolabeling). In contrast, retinoic acid treatment did not alter the morphology and connexin pattern of hair follicles or of sebaceous and sweat glands, and did not induce the expression of other connexins (C32, Cx37, Cx40) in either skin adnexae or epidermis. These observations suggest that the expression of two distinct connexins by interfollicular keratinocytes is related to selective changes in the differentiation program of epidermis that are induced by retinoic acid.

Download Full-text

Retinoic acid-induced modulation of rat liver transglutaminase and total polyamines in vivo

Biochemical Journal ◽

10.1042/bj2530033 ◽

1988 ◽

Vol 253 (1) ◽

pp. 33-38 ◽

Cited By ~ 20

Author(s):

M Piacentini ◽

L Fesus ◽

C Sartori ◽

M P Ceru

Keyword(s):

Retinoic Acid ◽

Acid Treatment ◽

Actinomycin D ◽

Tissue Transglutaminase ◽

Fold Increase ◽

Retinoic Acid Treatment ◽

Single Intraperitoneal Injection ◽

The Relationship ◽

Transglutaminase 3

The effect of a single intraperitoneal injection of retinoic acid on liver transglutaminase (EC 2.3.2.13) activity and total putrescine, spermidine and spermine was studied. The results demonstrate that: (1) transglutaminase activity is increased over control values as early as 4-6 h after treatment, reaching a maximum (2-fold increase) at 12 h and returning to control values at 36 h; (2) the retinoic acid-induced form of enzyme is the soluble tissue transglutaminase; (3) actinomycin D treatment does not completely inhibit the early (6 h) increase of activity, while suppressing that at 12 h; (4) the immunoassay of the soluble transglutaminase shows that, 6 h after treatment, there is no increase in the protein, whereas at 12 and 24 h a significant increase is observed; (5) putrescine, but not spermidine and spermine, increases (5-7-fold) 6 and 18 h after the retinoic acid treatment. The possibility also that the expression of soluble transglutaminase is modulated in vivo by retinoic acid and the relationship to polyamine levels are discussed.

Download Full-text