T46: Difference in composition of vaginal microbiota between women exhibiting spleen deficiency syndrome and women with damp-heat syndrome

2018 ◽  
Vol 80 ◽  
pp. 38-38
Keyword(s):  
Deficiency Syndrome ◽  
Vaginal Microbiota ◽  
Spleen Deficiency ◽  
Heat Syndrome ◽  
Spleen Deficiency Syndrome

scholarly journals Differences in the Composition of Vaginal Microbiota between Women Exhibiting Spleen-Deficiency Syndrome and Women with Damp-Heat Syndrome, Two of the Most Common Syndromes of Vaginitis in Traditional Chinese Medicine

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Wei Xian Lin ◽  
Xin Du ◽  
Li Lin Yang ◽  
Si Yun Chen ◽  
Wei Yu Qiu ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Deficiency Syndrome ◽  
Vaginal Microbiota ◽  
Vaginal Microbiome ◽  
Ancient Medicine ◽  
Spleen Deficiency ◽  
Significant Difference ◽  
Heat Syndrome ◽  
Spleen Deficiency Syndrome

Spleen-deficiency syndrome and damp-heat syndrome are the two most common syndromes of vaginitis in traditional Chinese medicine (TCM). Although it is known that the vaginal microbiota is closely associated with vaginitis, present studies have not fully elucidated the relationship between the composition of the vaginal microbiome and type of TCM syndrome because of the limitations in the present reductionist approaches. Samples of vaginal secretions were collected from patients with bacterial vaginitis and healthy subjects with spleen-deficiency syndrome and damp-heat syndrome, in order to analyze the constitution of the vaginal microflora using 16S rRNA sequencing methods that encompass taxonomic units, alpha diversity rarefaction curves, and principal component analyses. This prospective study indicated that there was a statistically significant difference in the composition of the vaginal microbiome between patients with spleen-deficiency syndrome and patients with damp-heat syndrome. Streptococcus was the dominant microbiota in patients with spleen-deficiency syndrome. This can serve as a biomarker for differentiating spleen-deficiency syndrome and damp-heat syndrome. In addition, as indicated by the findings on the samples, patients with bacterial vaginitis of dominant abundance in Pseudomonadaceae might be prone to manifest spleen-deficiency syndrome, while patients with bacterial vaginitis of dominant abundance in Prevotella might be prone to manifest damp-heat syndrome. These present findings can provide a new approach to acquire a scientific understanding of the syndromes of TCM, which in turn would benefit the development of personalized medicine, in terms of ancient medicine and complex biological systems.

scholarly journals Study on the Characteristics of Gut Microbiota in Chronic Hepatitis B Patients With Damp Heat Syndrome and Liver Depression and Spleen Deficiency Syndrome

2020 ◽  
Author(s):  
Kaiping Jiang ◽  
Qunfang Jiang ◽  
Xiaoai Mo ◽  
Jianhong Li ◽  
Hongtao Hu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rdna ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Deficiency Syndrome ◽  
Spleen Deficiency ◽  
Rdna Sequencing ◽  
Significant Difference ◽  
Heat Syndrome ◽  
Spleen Deficiency Syndrome

Abstract Background: To explore the potential biological characteristics of traditional Chinese medicine(TCM) syndromes in CHB patients is of great significance to improve the level of TCM treatment.This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common TCM Syndromes.Methods: According to the diagnostic criteria of Western medicine, TCM and screening exclusion criteria, 65 cases of CHB patients with damp heat syndrome and 28 cases of CHB patients with liver depression and spleen deficiency syndrome were finally included in the study. All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing. 16S rDNA of gut microbiota was sequenced using Illumina hiseq 2500 high-throughput sequencing platform.Based on the optimized sequence, OTU clustering analysis and taxonomic annotation were carried out. Results: The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients. Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales, Alphaproteobacteria,and Lachnospira were higher in the damp heat syndrome.LEfSe analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusions: The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.

scholarly journals Study on the Characteristics of Gut Microbiota in Chronic Hepatitis B Patients with Damp Heat Syndrome and Liver Depression and Spleen Deficiency Syndrome

2020 ◽  
Author(s):  
Kaiping Jiang ◽  
Qunfang Jiang ◽  
Xiaoai Mo ◽  
Jianhong Li ◽  
Hongtao Hu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rdna ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Deficiency Syndrome ◽  
Spleen Deficiency ◽  
Rdna Sequencing ◽  
Significant Difference ◽  
Heat Syndrome ◽  
Spleen Deficiency Syndrome

Abstract Background: To explore the potential biological characteristics of traditional Chinese medicine(TCM) syndromes in CHB patients is of great significance to improve the level of TCM treatment.This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common TCM Syndromes.Methods: According to the diagnostic criteria of Western medicine, TCM and screening exclusion criteria, 65 cases of CHB patients with damp heat syndrome and 28 cases of CHB patients with liver depression and spleen deficiency syndrome were finally included in the study. All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing. 16S rDNA of gut microbiota was sequenced using Illumina hiseq 2500 high-throughput sequencing platform.Based on the optimized sequence, OTU clustering analysis and taxonomic annotation were carried out. Results: The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients. Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales, Alphaproteobacteria,and Lachnospira were higher in the damp heat syndrome.LEfSe analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusions: The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.

scholarly journals Treatment of Spleen-Deficiency Syndrome With Atractyloside A From Bran-Processed Atractylodes lancea by Protection of the Intestinal Mucosal Barrier

2020 ◽  
Vol 11 ◽  
Author(s):  
Jiyuan Tu ◽  
Ying Xie ◽  
Kang Xu ◽  
Linghang Qu ◽  
Xiong Lin ◽  
...  
Keyword(s):  
Body Weight ◽  
Deficiency Syndrome ◽  
Mucosal Barrier ◽  
Enzyme Linked Immunosorbent Assay ◽  
Residual Rate ◽  
Intestinal Mucosal Barrier ◽  
Expression Levels ◽  
Spleen Deficiency ◽  
Atractylodes Lancea ◽  
Spleen Deficiency Syndrome

Atractylodes lancea (Thunb.) DC. (AL) is used in traditional Chinese medicine for the treatment of spleen-deficiency syndrome (SDS). Bran-processed Atractylodes lancea (BAL) has been found to be more effective than unprocessed AL. However, the compound in BAL active against SDS remains unclear. The pharmacological efficacy of BAL and its mechanism of action against SDS were investigated by HPLC-ELSD. Candidate compound AA (atractyloside A) in AL and BAL extracts was identified by HPLC-MS analysis. AA was tested in a rat model of SDS in which body weight, gastric residual rate, and intestinal propulsion were measured, and motilin (MTL), gastrin (GAS), and c-Kit were quantified by enzyme-linked immunosorbent assay. Potential targets and associated pathways were identified based on network pharmacology analysis. mRNA expression levels were measured by qRT-PCR and protein expression levels were measured by Western blot analysis and immunohistochemistry. AA increased body weight, intestinal propulsion, MTL, GAS, and c-Kit levels, while decreasing gastric residual volume and intestinal tissue damage, as same as Epidermal Growth Factor Receptor and Proliferating Cell Nuclear Antigen levels. Seventy-one potential pharmacologic targets were identified. Analysis of protein interaction, Gene Ontology (GO) functional analysis, pathway enrichment analysis, and docking and molecular interactions highlighted MAPK signaling as the potential signal transduction pathway. Validation experiments indicated that treatment with AA increased MTL, GAS, ZO-1, and OCLN levels, while reducing AQP1, AQP3, and FGF2 levels. In addition, phosphorylation of p38 and myosin light-chain kinase (MLCK) expression were inhibited. AA improved gastrointestinal function by protecting the intestinal mucosal barrier via inhibition of the p38 MAPK pathway. The results have clinical implications for the therapy of SDS.

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