Use of post-thaw semen quality parameters to predict fertility of water buffalo (Bubalus bubalis) bull during peak breeding season

Abstract. Horses are seasonal breeders with a natural breeding season beginning in spring and extending through midsummer. In this study, quantitative and qualitative parameters of chilled stallion semen were compared between fertile and subfertile stallions and between the breeding and the non-breeding season. Semen quality parameters compared included ejaculate volume, sperm concentration, total sperm number, sperm morphology, and computer-assisted semen analysis (CASA)-derived sperm movement characteristics obtained from two groups of warmblood stallions (n=8; four fertile stallions and four subfertile stallions), which differ in the seasonal pregnancy rate 80 %–90 % (fertile) vs. 40 %–60 % (subfertile). A total of 64 ejaculates were collected from the stallions (n=8; four in the breeding season and four in the non-breeding season of each stallion). No significant differences in the semen quality parameters between the fertile and the subfertile stallions in the non-breeding season were observed. However, in the breeding season the proportion of morphologically normal sperm, total motility, progressive motility, average path velocity (VAP), and curvilinear velocity (VCL) were significantly higher in the fertile group (P<0.05) when compared with the subfertile group. In addition, a significant seasonal variation in the proportion of morphological normal sperm was found in the fertile group between the breeding and the non-breeding season (P<0.05). Moreover, significant seasonal variations (P<0.05) in CASA parameters of mean VAP, straight line velocity (VSL), and beat-cross frequency (BCF) were observed in the fertile and the subfertile stallions, which tended to be lower in the non-breeding season. In conclusion, differences between the fertile and the subfertile stallions were observed only in the breeding season, and a few of CASA-derived parameters seemed to be significantly lower during the non-breeding season in both the fertile and the subfertile stallions.

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The Water Buffalo, Bubalus Bubalis, in Australia: Reproductive and Parent-Offspring Behaviour.

Wildlife Research ◽

10.1071/wr9790265 ◽

1979 ◽

Vol 6 (3) ◽

pp. 265 ◽

Cited By ~ 23

Author(s):

DG Tulloch

Keyword(s):

Breeding Season ◽

Water Buffalo ◽

Bubalus Bubalis ◽

Northern Territory ◽

Dry Season ◽

Family Group ◽

The Family

Water buffalo in the Northern Territory of Australia have a well defined breeding season in the wet or very early dry season. The birth of a calf is of major importance for the clan and for the family group of which that clan is a part. A number of calves are left in the care of an adult while their mothers go to graze. The female calf remains with its mother for many years, possibly for life, but at 2-3 y old the male calf is driven from the group by an adult bull when a cow in the group comes into oestrus. Most buffalo cows will readily adopt an orphan calf and within 8 days it is impossible to distinguish between the orphan and the group calves.

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Effects of the Seminal Plasma Zinc Content and Catalase Activity on the Semen Quality of Water Buffalo (Bubalus bubalis) Bulls

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2009.134.139 ◽

2009 ◽

Vol 12 (2) ◽

pp. 134-139 ◽

Cited By ~ 8

Author(s):

S.M. Alavi-Shou ◽

S. Asri Rezai ◽

M.H. Kh. Ansari ◽

A. Khaki

Keyword(s):

Seminal Plasma ◽

Catalase Activity ◽

Water Buffalo ◽

Semen Quality ◽

Zinc Content ◽

Bubalus Bubalis ◽

Plasma Zinc ◽

Quality Of Water

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A diagnostic method for gander selection in Zi geese (Anser cygnoides L.) and Rhin geese (Anser anser L.) using massage reaction and semen quality

Animal Production Science ◽

10.1071/an12387 ◽

2014 ◽

Vol 54 (5) ◽

pp. 569

Author(s):

S. J. Liu ◽

J. L. Li ◽

H. Li

Keyword(s):

Breeding Season ◽

Semen Quality ◽

Quality Parameters ◽

Anser Anser ◽

Laboratory Assessment ◽

Semen Collection ◽

Semen Volume ◽

Natural Breeding ◽

Egg Fertility ◽

Selection Of

The objective of this study was to score reactions to artificial semen collection procedures and to develop an efficient method for gander selection in two original geese breeds. One-hundred and three Zi geese and 110 Rhin geese were used. Gander reaction scores to massage and semen quality parameters were investigated in 10 Zi and 10 Rhin ganders during a complete reproductive season. The relationships between average gander massage reaction score and semen quality were evaluated. After natural mating, egg fertility comparison between Zi ganders selected by massage reaction scores and those not subjected to selection were investigated. Ganders exhibited large variations in artificial semen collection and semen characteristics between breeds. Only 30.1% (31/103) of Zi geese and 46.3% (51/110) of Rhin geese had stable positive massage reactions with semen ejaculation (P < 0.05). Average massage reaction score, ejaculate volume, spermatozoa concentration, sperm motility and semen quality factor for single ejaculate were significantly higher in Zi than in Rhin geese. There was a high correlation between average massage reaction score and semen volume, being 0.87 for Zi geese (P < 0.05) and 0.82 for Rhin (P < 0.05), and this correlation enabled pre-selection of ganders at the beginning of the breeding season. Reproductive efficiency could be improved with massage-selected ganders in natural breeding flocks. Examination of gander massage reaction scores combined with laboratory assessment of semen quality early in the breeding season is a feasible method for predicting fertilising capability of ganders.

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Superovulatory response of water buffalo (Bubalus bubalis) to gonadotrophins out of the breeding season

Italian Journal of Animal Science ◽

10.4081/ijas.2007.s2.626 ◽

2007 ◽

Vol 6 (sup2) ◽

pp. 626-628

Author(s):

J.L. Zambrano-Varón ◽

R.H. BonDurant

Keyword(s):

Breeding Season ◽

Water Buffalo ◽

Bubalus Bubalis

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Monitoring of libido and semen quality parameters in melatonin-treated French alpine bucks during the non-breeding season

Reproduction in Domestic Animals ◽

10.1111/rda.13003 ◽

2017 ◽

Vol 52 (6) ◽

pp. 953-961 ◽

Cited By ~ 3

Author(s):

S Vince ◽

H Valpotić ◽

V Berta ◽

S Milinković-Tur ◽

M Samardžija ◽

...

Keyword(s):

Breeding Season ◽

Semen Quality ◽

Quality Parameters

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Season movement and distribution of the sexes in the water buffalo, Bubalus bubalis, in the Northern Territory

Australian Journal of Zoology ◽

10.1071/zo9700399 ◽

1970 ◽

Vol 18 (4) ◽

pp. 399 ◽

Cited By ~ 17

Author(s):

DG Tulloch

Keyword(s):

Breeding Season ◽

Water Buffalo ◽

Bubalus Bubalis ◽

Black Soil ◽

Northern Territory ◽

Dry Season ◽

Coastal Plains ◽

Wet Season ◽

Large Numbers ◽

Northern Portion

Regular aerial and land surveys of the coastal plains and adjoining high ground in the northern portion of the Northern Territory between the Adelaide and Wildman Rivers showed variations in the number of buffalo on the plains. When there were very few buffalo on the plains there were large numbers on the adjoining high timbered country. During the wet season the plains flooded to varying depths and in those areas where the water was very deep all buffalo moved to higher ground. In the Northern Territory the wet season is the main breeding season, and at this time adult bulls were seen with cows in oestrus. During the dry season, which is the non-breeding season, the bulls and cows inhabited separate areas; the cows and their calves preferred those areas of the black soil plains adjoining high ground where there was water, shade, and some green feed. When rain fell at the end of the dry season, the buffalo moved to areas where it had rained.

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Quality of ram spermatozoa separated with modified swim up method

Bangladesh Veterinarian ◽

10.3329/bvet.v33i2.36459 ◽

2018 ◽

Vol 33 (2) ◽

pp. 62-70 ◽

Cited By ~ 2

Author(s):

A Hossain ◽

MM Islam ◽

F Naznin ◽

RN Ferdousi ◽

FY Bari ◽

...

Keyword(s):

Semen Quality ◽

Membrane Integrity ◽

Quality Parameters ◽

Fluid Medium ◽

Normal Morphology ◽

The Mean ◽

Mass Activity ◽

Fresh Semen ◽

Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

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