scholarly journals Seasonal variations in quantitative and qualitative sperm characteristics in fertile and subfertile stallions

2020 ◽  
Vol 63 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Yara Suliman ◽  
Frank Becker ◽  
Armin Tuchscherer ◽  
Klaus Wimmers
Keyword(s):  
Breeding Season ◽  
Seasonal Variations ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Computer Assisted ◽  
Normal Sperm ◽  
Straight Line ◽  
Significant Seasonal Variation

Abstract. Horses are seasonal breeders with a natural breeding season beginning in spring and extending through midsummer. In this study, quantitative and qualitative parameters of chilled stallion semen were compared between fertile and subfertile stallions and between the breeding and the non-breeding season. Semen quality parameters compared included ejaculate volume, sperm concentration, total sperm number, sperm morphology, and computer-assisted semen analysis (CASA)-derived sperm movement characteristics obtained from two groups of warmblood stallions (n=8; four fertile stallions and four subfertile stallions), which differ in the seasonal pregnancy rate 80 %–90 % (fertile) vs. 40 %–60 % (subfertile). A total of 64 ejaculates were collected from the stallions (n=8; four in the breeding season and four in the non-breeding season of each stallion). No significant differences in the semen quality parameters between the fertile and the subfertile stallions in the non-breeding season were observed. However, in the breeding season the proportion of morphologically normal sperm, total motility, progressive motility, average path velocity (VAP), and curvilinear velocity (VCL) were significantly higher in the fertile group (P<0.05) when compared with the subfertile group. In addition, a significant seasonal variation in the proportion of morphological normal sperm was found in the fertile group between the breeding and the non-breeding season (P<0.05). Moreover, significant seasonal variations (P<0.05) in CASA parameters of mean VAP, straight line velocity (VSL), and beat-cross frequency (BCF) were observed in the fertile and the subfertile stallions, which tended to be lower in the non-breeding season. In conclusion, differences between the fertile and the subfertile stallions were observed only in the breeding season, and a few of CASA-derived parameters seemed to be significantly lower during the non-breeding season in both the fertile and the subfertile stallions.

P–011 Automated sperm morphology assessment using artificial intelligence technology

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Agarwal ◽  
M K Panne. Selvam
Keyword(s):  
Artificial Intelligence ◽  
Predictive Value ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Ease Of Use ◽  
Computer Assisted ◽  
Diagnostic Use ◽  
Normal Sperm

Abstract Study question Can LensHooke X1 PRO semen analyzer be used to evaluate sperm morphology in men with infertility? Summary answer Morphology results generated by X1 PRO are highly reliable when normal sperm forms are ≥4% and therefore they can be reported in such cases . What is known already Most laboratories rely on manual evaluation of sperm morphology smears, which is a time-consuming procedure and its results are subjected to a relatively high variability. However, in recent years the computer-assisted semen analyzers are being increasingly used to evaluate sperm morphology. The X1 PRO semen quality analyzer was designed for in vitro diagnostic use to analyze sperm concentration, total, progressive and non-progressive motility as well as sperm morphology based on WHO 5th edition criteria. Evaluation of sperm morphology using X1 PRO based on AIOM (Artificial Intelligence Optical Microscopic)-based technology requires no fixation steps or staining unlike the manual method. Study design, size, duration This cross-sectional study used 31 semen samples from 8 normozoospermic healthy volunteers and 5 infertile men with a minimum abstinence period between 2 - 3 days. While the 8 healthy semen donors produced a total of 26 ejaculates, which were split into 88 aliquots, the 5 infertile patients produced 5 ejaculates that were split into 13 aliquots. Participants/materials, setting, methods A total of 101 aliquots were prepared from the native semen samples either by dilution or concentration using seminal plasma of the respective donors. Automated semen analysis was performed by the X1 PRO semen analyzer and the results of sperm morphology were compared with manual morphology results using Diff-Quik staining. Statistical analysis was carried out to calculate the positive predictive value (PPV) and negative predictive value (NPV) of X1 PRO semen analyzer. Main results and the role of chance The X1 PRO sperm morphology results show a weak non-significant (P = 0.2441) correlation (r = 0.119) with the manual results. However, X1 PRO demonstrated a high PPV (97.7%) and a low NPV (9.1%) for correct assessment of sperm morphology (≥4%) when compared to manual results. Due to its high PPV, laboratories can report the morphology results generated by X1 PRO in all such cases when normal sperm forms are ≥4%. However, a manual evaluation is necessary in patients with abnormal morphology (&lt;4%). Limitations, reasons for caution One of the limitation of this study is that X1 PRO morphology values did not correlate with manual results. The low NPV seen in our study is due to the inclusion of very few samples with abnormal sperm forms (&lt;4%) in the analysis. Wider implications of the findings: The X1 PRO’s combination of speed, ease of use, accuracy and portability makes it a good choice of device for small medical offices to large IVF centers. High PPV of X1 PRO allows it to correctly identify normal sperm forms for diagnostic use. Trial registration number 18–771

scholarly journals Fresh semen characteristics in captive accipitrid and falconid birds of prey

2020 ◽  
Vol 89 (3) ◽  
pp. 291-300
Author(s):  
Vladimír Piaček ◽  
Jan Zukal ◽  
Veronika Seidlová ◽  
Tomáš Heger ◽  
Monika Němcová ◽  
...  
Keyword(s):  
Breeding Season ◽  
Semen Quality ◽  
Semen Analysis ◽  
Birds Of Prey ◽  
Computer Assisted ◽  
Assisted Reproductive Technique ◽  
Peregrine Falcons ◽  
Semen Characteristics ◽  
Northern Goshawks ◽  
Fresh Semen

Artificial insemination (AI) is the most frequently used assisted reproductive technique for captive propagation of rare avian species. As semen quality is critical for reproductive success, baseline data are needed for evaluating and selecting the best male bird donors. To this end, we used computer-assisted semen analysis to assess male eastern imperial eagles (n = 7), northern goshawks (n = 24) and peregrine falcons (n = 20). While imperial eagles and northern goshawks donate ejaculate voluntarily, peregrine falcons required cloacal massage. Eight peregrine falcon females were inseminated with semen from eight males, with fresh ejaculates (15 to 50 µl) applied to the pars uterina of the oviduct immediately after collection and examination. All females were inseminated within 2 h of laying an egg. A fertilization rate of 70% was achieved using this method. Minimum semen characteristics associated with egg fertilization included a semen concentration of 115.12 × 106/ml, 33.52% total motility, 1.92% spermatozoa with progressive motility and 0.17% with rapid motility. Comparative data on spermatozoa concentration and kinematics suggest that eastern imperial eagles concentrate on high quality semen investment at the start of the breeding season, northern goshawks compensate for a decrease in motility-associated parameters with increased semen concentration and peregrine falcons maintain semen production standards throughout the breeding season. Our data show that, in birds of prey, levels of egg fertilization following AI with fresh semen can be almost as successful as after natural mating.

scholarly journals The effect of varicocele on semen quality in boars exposed to heat stress1

2020 ◽  
Vol 4 (1) ◽  
pp. 293-298
Author(s):  
Tasha R Gruhot ◽  
Lea A Rempel ◽  
Brett R White ◽  
Benny E Mote
Keyword(s):  
Heat Stress ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Computer Assisted ◽  
Swine Industry ◽  
Maternal Line ◽  
Component Trait ◽  
Potential Marker ◽  
Quality Issues

Abstract Semen quality has a dramatic impact on reproductive efficiency in the swine industry, influencing both conception rate and litter size. The objective of this study was to assess whether the presence of varicocele hinders semen quality in both thermoneutral and heat stress (HS) conditions. At approximately 6 mo of age, ultrasonography was used to measure left and right pampiniform plexus area in order to detect varicocele in maternal line boars at the University of Nebraska–Lincoln. Between 10 and 12 mo of age, semen was collected from each boar (n = 28) twice weekly. Boars were collected under thermoneutral conditions, were then heat stressed for 7 d to exacerbate any semen quality issues, and semen was collected post-HS for 6 wk. Sperm characteristics were determined by computer-assisted semen analysis. The presence of varicocele had a significant effect on sperm concentration (P = 0.04) and trended toward significance for mean sperm head area (P = 0.06) throughout the duration of the study. An interaction existed between varicocele and collection time point at weeks 2–5 post-HS for distal droplet percentage, suggesting that boars with varicocele were possibly more susceptible to heat-stress-induced semen quality issues than boars without varicocele. Moreover, semen quality was reduced in boars with versus without varicocele under both thermoneutral and HS conditions. Therefore, detection of varicocele by ultrasound could represent a potential marker of fertility in young boars or as a component trait in selection indices for fertility.

scholarly journals 221 Effect of age and rut period on the semen quality in reindeer (Rangifer tarandus)

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 48-49
Author(s):  
Elena Nikitkina ◽  
Artem Musidray ◽  
Anna Krutikova ◽  
Svetlana Timofeeva ◽  
Kirill Plemyashov ◽  
...  
Keyword(s):  
Rangifer Tarandus ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
The Arctic ◽  
Computer Assisted ◽  
Reindeer Herding ◽  
Russian Science ◽  
Effect Of Age

Abstract Reindeer herding is a traditional activity of the indigenous peoples of the Arctic. Assisted reproductive technology in reindeer herding are under development. This is due to the difficulty of collecting reindeer sperm (Rangifer tarandus) in the Arctic. The aim of the work was to study the influence of age and rut period on the quality of reindeer (Rangifer tarandus) sperm. Semen was collected in Taimyr in autumn 2017, 2018. Ejaculate volume, total (TM) and progressive motility (PM) and sperm concentration were evaluated. TM, PM and sperm concentration were measured by CASA (computer-assisted semen analysis). Sperm was not found in caudae epididymidae in males at the age of six months. The sperm was collected only from caudae epididymidae from males aged 1.5 and 2.5 years. Collecting of ejaculated semen is possible in males from 3.5 years of age. The volume of ejaculate increased from 0.3±0.09 ml (mean±SD) at the age of 3.5 years to 0.7±0.09 ml at the age of 5.5 years and older (P &lt; 0.05). Concentration of cells increased by 2 times in older males. It is known that reindeer sperm can be collected only during the rut. At the beginning and end of the rut, the collection of sperm by electroejaculation was not always successful, sperm was collected in 3 of 10 cases, while in the middle of the rut in 9 of 10 cases. There was no significant effect of the rut period on the ejaculate volume and sperm concentration. The lowest TM (51.1±2.16%) and PM (33.5±7.90%) were at the beginning of the rut, the highest at the end (73.0±5.02% and 59.0 ± 6,30%) (P &lt; 0.01). Thus, the effect of age and rutting period on the reindeer sperm was observed. Authors acknowledge financial support from Russian Science Foundation, Grant No:17-16-01023.

scholarly journals Assessment of semen quality in infertile dogs using computer-assisted sperm analysis by the Hamilton-Thorne Semen Analyser

2013 ◽  
Vol 57 (3) ◽  
pp. 429-432 ◽  
Author(s):  
Anna Domosławska ◽  
Sławomir Zduńczyk ◽  
Wojciech Niżański ◽  
Tomasz Janowski
Keyword(s):  
Semen Quality ◽  
Prostate Gland ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Computer Assisted ◽  
Normal Morphology ◽  
Sperm Analysis ◽  
History Of ◽  
Possible Cause ◽  
Motility Parameters

Abstract Semen quality parameters of infertile and fertile dogs were compared. Sperm concentration and semen motility parameters were measured by the Hamilton-Thorne Semen Analyser IVOS 12.3. The spermatozoal morphology and the percentage of live spermatozoa were examined microscopically. Forty-six dogs of various breeds were examined. Twenty dogs had a conception failure within last year. These dogs had a history of prior normal fertility. Twenty six fertile dogs served as control. All animals underwent a clinical examination as well as ultrasonography. Sperm concentration was significantly lower in infertile dogs than in fertile dogs. For most determined motility parameters (MOT, PMOT, VAP, VSL, VCL, BCF, RAPID, STATIC) significant differences between infertile and fertile dogs were found. The percentage of spermatozoa with normal morphology also significantly differed between both groups. Ultrasonography of the prostate gland and testes revealed no pathological conditions. The testicular degeneration was assumed to be a possible cause of infertility in these dogs. The present study showed that the most sperm CASA motility parameters were significantly lower in infertile dogs in comparison to the fertile ones, and confirmed the usefulness of the Hamilton-Thorne Semen Analyser for a quick and objective analysis of sperm concentration and motility in dogs.

scholarly journals Correlation between the sperm characteristics evaluated by a computerized system (CASA) and the reproductive performance of sows

2020 ◽  
Vol 9 (6) ◽  
pp. e09963389
Author(s):  
Emanuelle Maria Gottardi ◽  
Thaisa Campos Marques ◽  
Karen Martins Leão ◽  
Francisco Ribeiro de Araújo Neto ◽  
Leidiane Gonçalves Fernandes
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Head Displacement ◽  
Sperm Characteristics ◽  
Flagellar Beat ◽  
Straight Line ◽  
Computerized System ◽  
Farrowing Rate

Computer-assisted semen analysis (CASA) systems have been one the most used tools to evaluate sperm kinetics. The objective of this research was to estimate the correlation between sperm motility characteristics evaluated by CASA during 72 hours of cooling with the farrowing rate (FR) and total number of piglets born (TNB) after artificial insemination. Multiparous sows (n=464) were inseminated with semen from seven boars (19.6 ± 1.3 ejaculates/male). Sperm motility parameters were determined immediately after dilution and after 24, 48 and 72 hours of cooling at 15°C: total motility (TM-%), progressive motility (PM-%), curvilinear velocity (VCL-μm/s), straight line velocity (VSL-μm/s), average path velocity (VAP-µm/s), amplitude of lateral head displacement (ALH-µm), flagellar beat cross frequency (BCF-Hz), straightness (STR-%) and linearity (LIN-%). Pearson’s correlation coefficient was applied to analyze the data and the comparison of the means of the sperm characteristics between the boars was done by Tukey’s test at 5% probability. TM and PM at time zero (T0) were significant and had a moderate to high correlation with FR and TNB. After 72 hours of refrigeration, the semen quality was reduced and showed a significant and low correlation of the TM and PM with these same parameters. The boar presenting the lowest value of TM and PM after dilution obtained lower FR and TNB. In conclusion, computer-assisted semen analysis soon after dilution can be used to predict fertility of boars.

scholarly journals Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria

2019 ◽  
Vol 15 (2-1) ◽  
pp. 303-306
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Ma’aruf Bashir Sani
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Indigenous Breed ◽  
Normal Sperm ◽  
Abnormal Sperm ◽  
Significant Difference ◽  
Semen Characteristics ◽  
Genetically Improved ◽  
Abdominal Massage

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ±  to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.

scholarly journals Variation of Semen Quality Among Three Generations (F1, F2 and F3) of Holstein Friesian Upgraded Breeding Bulls of Bangladesh

2020 ◽  
Vol 7 (3) ◽  
pp. 457-463
Author(s):  
Faruk Hossain ◽  
Md Golam Sorowar ◽  
Sharmin Akter Suma ◽  
Abdullah Al Mansur ◽  
Md Mahbubul Hoque ◽  
...  
Keyword(s):  
First Generation ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Quality Parameter ◽  
Microsoft Excel ◽  
Three Generations ◽  
Holstein Friesian ◽  
Recorded Data ◽  
Mass Activity

The experiment was conducted in a well-known private livestock research and development farm in Bangladesh to find out the variation of semen quality parameter among three generations (F1, F2 and F3) of Holstein Friesian upgraded breeding bulls. A total of 312 ejaculates were collected from 6 upgraded breeding bullsthrought the experimental year. The recorded data were summarized using Microsoft Excel 2010 and analyzed using GraphPad Prism 5 softwere. Out of the 312 ejaculates, 273 (87.50%) were found to be creamy in color followed by 26 (8.33%) and 13 (4.17%) as yellowish and watery, respectively. Generation had significant (P<0.05) effect on ejaculate volume, consistancy, mass activity, sperm concentration, initial and post freezing motility. The highest (7.389±0.19ml) and the lowest (5.156±0.13ml) volume of semen were found in third (F3) and first generation (F1), respectively. The mass activity ranges from 3.74±0.04 to 4.30±0.05. Sperm concentration and pH varied insignificantly (p>0.05) but initial motility and post freezing motility had the significant differences among the three generations. Initial motility ranges from 75.87±0.32 to 78.40±0.38 percent and the post freezing motility ranges from 50.38±0.41 to 52.16±0.43 percent. It could be concluded that most of the semen quality parameters were influenced by generation and freezing. Semen characteristics were better in F2 followed by F3 and F1 generation in upgraded Holstein Friesian bulls. Res. Agric., Livest. Fish.7(3): 457-463,  December 2020

scholarly journals Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

scholarly journals Evaluation of sperm motility and velocity of different genetic groups of bucks using Computer-Assisted Sperm Analyzer

2020 ◽  
Vol 48 (2) ◽  
pp. 67-74
Author(s):  
MR Islam ◽  
MKU Talukder ◽  
MS Rahman ◽  
SS Husain
Keyword(s):  
Semen Quality ◽  
Computer Assisted ◽  
Scrotal Circumference ◽  
Future Performance ◽  
Genetic Groups ◽  
Straight Line ◽  
Semen Volume ◽  
Path Distance ◽  
Positive Correlations ◽  
Better Than

Semen quality assessment is a prime prerequisite for any breeding programme. Computer-Assisted Sperm Analyzer (CASA) can measure the semen quality very accurately and sharply. The research was conducted at American Dairy Limited (ADL) at Vangnahati, Sreepur, Gazipur. A total of 120 ejaculates were collected from three genetic groups of bucks namely Black Bengal (BB), Boer and Jamunapari (JP) with a view to characterize and compare the semen motility and velocity parameters using Computer- Assisted Sperm Analyser. Significantly (p<0.05) higher ejaculate volume was found in Boer buck (1.73±0.16 ml) with the lower in BB buck (0.98±0.14), respectively. But significantly (p<0.05) higher total, forward progressive and fast motility was found in BB (55.47±3.17, 47.23±2.74 and 37.62±2.40%) and lower in JP (40.67±3.37, 30.97±2.91 and 21.81±2.55%), respectively. Significantly (p<0.01) fastest straight line and average path distance was observed in Boer bucks (12.29±1.63 and 15.89±1.95 μm) than Black Bengal and Jamunapari bucks (4.38±1.19 and 7.26±1.57; and 3.14±1.27 and 8.42±1.95 μm), respectively. The distance curved line (DCL) and distance straight line (DSL) was significantly (p<0.05) higher in Boer (29.13±4.87, 12.29±1.63 and 15.89±1.95 _m/sec) and lower in BB (22.07±4.36 and 7.26±1.57 _m/sec), respectively. In case of curvilinear velocity, straight line velocity and average path velocity value was significantly (p<0.01) higher in Boer (127.08±13.88, 50.01±7.68 and 68.13±7.25 _m/sec) with lower in JP (62.01±12.77, 15.75±7.35 and 22.39±9.42) where as linearity and straightness was higher in Boer and lower in BB (p>0.05), respectively. The scrotal circumference had positive correlations with semen volume and curvilinear velocity for all the genotypes. It was concluded that semen of Boer bucks found consistently better than other two genetic groups of bucks. Further, competitive studies with large sample may be performed to evaluate the breed specific differences in available goat breeds. I think this study also disclosed the some rare parameters of semen quality by using Computer-Assisted Sperm Analyzer which was impossible in manually. These results of the present study could be an authentic guideline in future performance tests of different genetic groups of bucks. Bang. J. Anim. Sci. 2019. 48 (2): 67-74

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