Effects of the Seminal Plasma Zinc Content and Catalase Activity on the Semen Quality of Water Buffalo (Bubalus bubalis) Bulls

Abstract The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

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Use of post-thaw semen quality parameters to predict fertility of water buffalo (Bubalus bubalis) bull during peak breeding season

Andrologia ◽

10.1111/and.12639 ◽

2016 ◽

Vol 49 (4) ◽

pp. e12639 ◽

Cited By ~ 10

Author(s):

H. Ahmed ◽

S. M. H. Andrabi ◽

M. Anwar ◽

S. Jahan

Keyword(s):

Breeding Season ◽

Water Buffalo ◽

Semen Quality ◽

Bubalus Bubalis ◽

Quality Parameters

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Altered Expression Pattern of Major Seminal Plasma Protein Genes in Seminal Vesicles of Water Buffalo (Bubalus bubalis)

Proceedings of the National Academy of Sciences India Section B Biological Sciences ◽

10.1007/s40011-015-0629-3 ◽

2015 ◽

Vol 87 (3) ◽

pp. 655-661

Author(s):

Ashish Shende ◽

S. K. Bhure ◽

Harikrishna Pillai ◽

Muhammed Kutty V. H ◽

Manish Mahaver ◽

...

Keyword(s):

Expression Pattern ◽

Plasma Protein ◽

Seminal Plasma ◽

Water Buffalo ◽

Bubalus Bubalis ◽

Seminal Vesicles ◽

Altered Expression ◽

Altered Expression Pattern

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Label-free proteome of water buffalo (Bubalus bubalis ) seminal plasma

Reproduction in Domestic Animals ◽

10.1111/rda.13206 ◽

2018 ◽

Vol 53 (5) ◽

pp. 1243-1246 ◽

Cited By ~ 6

Author(s):

Mayara F. Brito ◽

Patrícia A. Auler ◽

Guilherme C. Tavares ◽

Cristiana P. Rezende ◽

Gabriel M. F. Almeida ◽

...

Keyword(s):

Seminal Plasma ◽

Water Buffalo ◽

Bubalus Bubalis ◽

Label Free

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First study of water quality in the San Camilo and Mojahuevo estuaries located in Guayas for being used in aquaculture

Acta periodica technologica ◽

10.2298/apt2152055r ◽

2021 ◽

pp. 55-62

Author(s):

René Rodríguez-Grimón ◽

Juan Valenzuela-Cobos ◽

Juan Erazo-Delgado ◽

Narvaez Teran ◽

María Garcés-Moncayo ◽

...

Keyword(s):

Water Quality ◽

Rainy Season ◽

Phenolic Content ◽

Oxygen Demand ◽

Zinc Content ◽

Fats And Oils ◽

Ammonia Content ◽

Quality Of Water ◽

Productive Process

The water quality of the San Camilo and Mojahuevo estuaries was monitored in the months of April (rainy season) and June (dry season) of 2016. The water of the Mojahuevo estury presented in both seasons the lowest values of turbidity between 17.20 and 33.70 UNT, biochemical oxygen demand being in a range between 14.00 and 19.00 mg/L, zinc content (0.02-0.05 mg/L) and copper content (0.07 mg/L), ammonia content (0.21-0.35 mg/L), fats and oils (0.23-0.29 mg/L), phosphates (0.39-2.34 mg/L) and nitrates (0.84-2.29 mg/L), while the water of the two estuaries San Camilo and Mojahuevo (rainy season) showed similar phenolic content (0.019 mg/L). The results showed that the Mojahuevo estuary presented the high quality of water recommended for being used in productive process like aquaculture due to the low presence of pollutants.

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Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen

Reproduction Fertility and Development ◽

10.1071/rd17044 ◽

2018 ◽

Vol 30 (3) ◽

pp. 563 ◽

Cited By ~ 8

Author(s):

Alexandra Usuga ◽

Benjamín A. Rojano ◽

Giovanni Restrepo

Keyword(s):

Seminal Plasma ◽

Linear Models ◽

Semen Quality ◽

Membrane Integrity ◽

Secretory Protein ◽

Enzyme Linked Immunosorbent Assay ◽

Nucleotide Polymorphisms ◽

Seminal Plasma Proteins ◽

High Level

Contribution of seminal plasma proteins to semen freezability has been reported in several species, suggesting these proteins as genetic markers. The aim of this study was to evaluate the relationship between cysteine-rich secretory protein-3 (CRISP-3) and some of its single-nucleotide polymorphisms (SNPs) with post-thawing semen quality in stallions. DNA was obtained from 100 stallions, regions of interest were amplified by polymerase chain reaction and sequenced. Evaluated SNPs within the equine CRISP-3 gene were CRISP3c.+199A > G (SNP1), CRISP3c.+566C > A (SNP2), CRISP3c.+622G > A (SNP3) and CRISP3c.+716A > G (SNP4). CRISP-3 protein content in seminal plasma was determined by enzyme-linked immunosorbent assay. Semen from 30 stallions was cryopreserved and post-thaw motility, kinetics, abnormal morphology (AM), sperm vitality (SV) and membrane integrity (MI) were evaluated. Generalized linear models were fitted and means were compared using Tukey’s test. Correlation and regression analyses were performed. For SNP1 and SNP3, the AA genotype had the highest results for motility and MI; for SNP2, the best results for motility and AM were obtained with the CC genotype. For SNP4, the GG genotype had the lowest results, except for MI. A high level of CRISP-3 protein in seminal plasma had the best results for motility, kinetics, SV and AM. In conclusion, there was a relationship between CRISP-3 genotype and seminal plasma protein and post-thawing semen quality in stallions.

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