Influence of light on biomass and lipid production in microalgae cultivation

Lipids produced by microalgal biomass can be grouped into nonpolar lipids and polar lipids, which can be easily converted into biofuels. Microalgal samples were collected from three different ponds of Bangalore and cultured in the laboratory to find the effect of different pH, temperature and media on the production of biomass and lipids. Among these, pH-9, temperature -25°C and Beneck’s media was most suitable for production of biomass (35.80 g/L) and lipids from the isolated microalgae Chlorella sp. compare to Chladospora sp. (13.33 g/L). Chlorella sp. Showed 0.32 (OD) at pH-9, 0.43 (OD) at temperature-25°C and 2.94 (OD) in Beneck’s media. Our result revealed that nutrient supply along with measured variables affects the production of biomass and lipids in different microalgae.DOI: http://dx.doi.org/10.3126/ijls.v8i2.10227 International Journal of Life Sciences Vol.8(2): 2014; 13-17

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Statistical Approach of Nutrient Optimization for Microalgae Cultivation

E3S Web of Conferences ◽

10.1051/e3sconf/202014103009 ◽

2020 ◽

Vol 141 ◽

pp. 03009

Author(s):

Pichayatorn Bunkaew ◽

Sasithorn Kongruang

Keyword(s):

Light Intensity ◽

Cobalt Chloride ◽

Lipid Production ◽

Biodiesel Production ◽

Mixotrophic Cultivation ◽

Microalgae Cultivation ◽

Cultivation Time ◽

Chlorella Sp ◽

Chloride Hexahydrate ◽

Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

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A Review on Synchronous Microalgal Lipid Enhancement and Wastewater Treatment

Energies ◽

10.3390/en14227687 ◽

2021 ◽

Vol 14 (22) ◽

pp. 7687

Author(s):

Visva Bharati Barua ◽

Mariya Munir

Keyword(s):

Wastewater Treatment ◽

Efficient Solution ◽

Culture Media ◽

Lipid Production ◽

Cost Effective ◽

Biofuel Production ◽

Microalgae Cultivation ◽

Photosynthetic Eukaryotes ◽

Biomass Enhancement ◽

Microalgae Biomass

Microalgae are unicellular photosynthetic eukaryotes that can treat wastewater and provide us with biofuel. Microalgae cultivation utilizing wastewater is a promising approach for synchronous wastewater treatment and biofuel production. However, previous studies suggest that high microalgae biomass production reduces lipid production and vice versa. For cost-effective biofuel production from microalgae, synchronous lipid and biomass enhancement utilizing wastewater is necessary. Therefore, this study brings forth a comprehensive review of synchronous microalgal lipid and biomass enhancement strategies for biofuel production and wastewater treatment. The review emphasizes the appropriate synergy of the microalgae species, culture media, and synchronous lipid and biomass enhancement conditions as a sustainable, efficient solution.

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Microalgae cultivation as tertiary unit operation for treatment of pharmaceutical wastewater associated with lipid production

Bioresource Technology ◽

10.1016/j.biortech.2016.04.101 ◽

2016 ◽

Vol 215 ◽

pp. 117-122 ◽

Cited By ~ 26

Author(s):

Manupati Hemalatha ◽

S. Venkata Mohan

Keyword(s):

Lipid Production ◽

Unit Operation ◽

Microalgae Cultivation ◽

Pharmaceutical Wastewater

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Microalgae cultivation in palm oil mill effluent (POME) for lipid production and pollutants removal

Energy Conversion and Management ◽

10.1016/j.enconman.2018.08.057 ◽

2018 ◽

Vol 174 ◽

pp. 430-438 ◽

Cited By ~ 24

Author(s):

Wai Yan Cheah ◽

Pau Loke Show ◽

Joon Ching Juan ◽

Jo-Shu Chang ◽

Tau Chuan Ling

Keyword(s):

Palm Oil ◽

Lipid Production ◽

Palm Oil Mill Effluent ◽

Microalgae Cultivation ◽

Palm Oil Mill ◽

Pollutants Removal

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A Comparative Review on Different Trophic Modes and Usable Carbon Sources for Microalgae Cultivation, Approaching to Optimize Lipid Production in Trade Scale

Systematic Bioscience and Engineering ◽

10.37256/sbe.112021293 ◽

2020 ◽

pp. 16-26

Author(s):

Zahra Lari ◽

Fatemeh Khosravitabar

Keyword(s):

Carbon Source ◽

Large Scale ◽

Lipid Production ◽

Carbon Sources ◽

Point Of View ◽

Microalgae Cultivation ◽

Lipid Yield ◽

Comparative Review

Microalgae are considered as an outstanding feedstock to produce high value lipid products like biodiesel and biomedicine. Reaching commercial maturity in this field is possible in the case of maximizing lipid yield and minimizing prime costs. In order to clarify the best features of carbon source (for microalgae cultivation) to reach optimum efficiency of biomass and lipid production, this paper reviews the merits and demerits of different trophic modes as well as type and concentration of carbon source. Furthermore carbon supplementation for large scale microalgae cultivation and lipid production is discussed as an economical point of view.

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Construction of a tank in pilot scale for microalgae cultivation aiming lipid production

Revista dos Trabalhos de Iniciação Científica da UNICAMP ◽

10.20396/revpibic2620181362 ◽

2019 ◽

Author(s):

Vitor Bezerra Lopes ◽

Leonardo Vasconcelos Fregolente ◽

Luisa Rios Pinto ◽

Gabriela Filipinii Ferreira

Keyword(s):

Lipid Production ◽

Lipid Extraction ◽

Scale Up ◽

Pilot Scale ◽

Industrial Scale ◽

Microalgae Cultivation ◽

The Future ◽

Mixotrophic Conditions

The cultivation of two microalgae species was conducted under mixotrophic conditions, followed by lipid extraction to evaluate possible applications. Monitoring its growth, it is possible to optimize this process to increase both biomass and lipid productions, and in the future, gradually scale up to the possible use of this study in a pilot or industrial scale.

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The Effect of Nutrients Mixture on The Biomass and Lipid Production from Microalgae Botryococcus braunii Mutated by UV-C Rays

Jurnal Rekayasa Proses ◽

10.22146/jrekpros.69228 ◽

2021 ◽

Vol 15 (2) ◽

pp. 182

Author(s):

Thea Prastiwi Soedarmodjo ◽

Hakun Wirawasista Aparamarta ◽

Arief Widjaja

Keyword(s):

Large Scale ◽

Lipid Production ◽

Botryococcus Braunii ◽

Raw Material ◽

Triple Superphosphate ◽

Microalgae Cultivation ◽

Mutation Process ◽

Cultivation Process ◽

Uv C ◽

Microalgae Growth

Nutrient is one of the most important factors in the growth of microalgae. This research was conducted to study the effect of nutrient mixture on the biomass and lipid production of Botryococcus braunii. Microalgae B. braunii was cultivated in the commercial nutrient medium of agricultural fertilizer combinations of ammonium sulphate (ZA), urea, and triple superphosphate (TSP). Before the cultivation process, B. braunii was exposed to UV-C rays (254 nm) for 3 minutes. The concentration and type of fertilizer as a nitrogen source divided into four types of mixtures, namely FM-1, FM-2, FM-3, and FM-4 were compared with Walne nutrients to study their effects on microalgae growth and lipids. FM-1 consisting of 150 mg/L of ZA, 7.5 mg/L of urea, and 25 mg/L of TSP led to the best growth for native and mutated microalgae strains compared to Walne nutrients and other nutrient mixtures. The mutated microalgae showed less growth than the native microalgae strains. However, the mutation process significantly increased the lipid content in the microalgae. In native microalgae strains, FM-4 consisting of 136.3 mg/L of urea and 50 mg/L of TSP produced the lowest lipid at 8.96%. After being exposed to UV-C rays, the lipids in FM-4 medium increased to 55.11%. The results show that the use of commercial fertilizers and exposure to UV-C rays on microalgae have high potential in preparing lipids as raw material for biodiesel which can be effectively applied in large-scale microalgae cultivation.

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