Characterization of eomesodermin and T-bet expression by allostimulated CD8+
 T cells of healthy volunteers and kidney transplant patients in relation to graft outcome

Predictive biomarkers of acute rejection (AR) are lacking. Pre-transplant expression of CD45RC on blood CD8+ T cells has been shown to predict AR in kidney transplant (KT) patients. The objective of the present study was to study CD45RC expression in a large cohort of KT recipients exposed to modern immunosuppressive regimens. CD45RC expression on T cells was analyzed in 128 KT patients, where 31 patients developed AR, of which 24 were found to be T-cell mediated (TCMR). Pre-transplant CD4+ and CD8+ CR45RChigh T cell proportions were significantly higher in patients with AR. The frequency of CD45RChigh T cells was significantly associated with age at transplantation but was not significantly different according to gender, history of transplantation, pre-transplant immunization, and de novo donor specific anti-Human Leucocyte Antigen (HLA) antibody. Survival-free AR was significantly better in patients with CD8+ CD45RChigh T cells below 58.4% (p = 0.0005), but not different according to CD4+ T cells (p = 0.073). According to multivariate analysis, CD8+ CD45RChigh T cells above 58.4% increased the risk of AR 4-fold (HR 3.96, p = 0.003). Thus, pre-transplant CD45RC expression on CD8+ T cells predicted AR, mainly TCMR, in KT patients under modern immunosuppressive therapies. We suggest that CD45RC expression should be evaluated in a prospective study to validate its usefulness to quantify the pre-transplant risk of AR.

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Characterization of CD28−CD4+ T cells in living kidney transplant patients with long-term allograft acceptance

Human Immunology ◽

10.1016/s0198-8859(01)00353-6 ◽

2001 ◽

Vol 62 (12) ◽

pp. 1335-1345 ◽

Cited By ~ 8

Author(s):

Masashi Kato ◽

Tetsuya Matsuguchi ◽

Yoshinari Ono ◽

Ryohei Hattori ◽

Shinichi Ohshima ◽

...

Keyword(s):

T Cells ◽

Kidney Transplant ◽

Cd4 T Cells ◽

Transplant Patients ◽

Kidney Transplant Patients

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ANALYSIS OF ALLOSPECIFIC T CELLS IN KIDNEY TRANSPLANT PATIENTS ON VARIOUS IMMUNOSUPPRESSIVE PROTOCOLS.

Transplantation Journal ◽

10.1097/00007890-200607152-01876 ◽

2006 ◽

Vol 82 (Suppl 2) ◽

pp. 692

Author(s):

&NA;

Keyword(s):

T Cells ◽

Kidney Transplant ◽

Transplant Patients ◽

Kidney Transplant Patients

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Molecular epidemiology of blaCTX-M gene-producing uropathogenic Escherichia coli among Iranian kidney transplant patients: clonal dissemination of CC131 and CC10

Annals of Clinical Microbiology and Antimicrobials ◽

10.1186/s12941-021-00470-7 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Mehrdad Halaji ◽

Shahrzad Shahidi ◽

Behrooz Ataei ◽

Abdolamir Atapour ◽

Awat Feizi ◽

...

Keyword(s):

Escherichia Coli ◽

Kidney Transplant ◽

Phylogenetic Group ◽

Control Group ◽

M Gene ◽

Mlst Analysis ◽

Phylogenetic Characterization ◽

Transplant Patients ◽

Kidney Transplant Patients

Abstract Background This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing blaCTX-M gene. Methods To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing blaCTX-M gene. Results According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B2, followed by group D (18.9%), and group A (13.5%). Furthermore, except for phylogenetic group C, there was no significant correlation between phylogenetic distribution in KTPs and non-KTPs. Additionally, MLST analysis of blaCTX-M carrying isolates identified 18 unique sequence types (ST) the most common of which was ST131 (24.1%), followed by ST1193 (10.3%), while fourteen STs were detected only once. Conclusions The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.

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