scholarly journals Critical defect healing assessment in rat calvaria using a titanium‐reinforced polytetrafluoroethylene membrane

2020 ◽  
Vol 31 (S20) ◽  
pp. 96-96
Author(s):  
Henrique Hadad ◽  
Bruno Chies ◽  
Laís Kawamata Jesus ◽  
Ana Flávia Piquera Santos ◽  
Francisley Ávila Souza ◽  
...  
Keyword(s):  
Defect Healing ◽  
Rat Calvaria ◽  
Critical Defect ◽  
Healing Assessment

scholarly journals Critical Defect Healing Assessment in Rat Calvaria Filled with Injectable Calcium Phosphate Cement

2019 ◽  
Vol 10 (2) ◽  
pp. 21 ◽  
Author(s):  
Luis Eduardo Schmidt ◽  
Henrique Hadad ◽  
Igor Rodrigues de Vasconcelos ◽  
Luara Teixeira Colombo ◽  
Rodrigo Capalbo da Silva ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Blood Clot ◽  
Biological Properties ◽  
Autogenous Bone ◽  
Calvarial Bone ◽  
Critical Size Defects ◽  
Post Surgery ◽  
Rat Calvaria ◽  
Healing Assessment

(1) Background: The tissue engineering field has been working to find biomaterials that mimic the biological properties of autogenous bone grafts. (2) Aim: To evaluate the osteoconduction potential of injectable calcium phosphate cement implanted in critical defects in rat calvaria. (3) Methods: In the calvarial bone of 36 rats, 7-mm diameter critical size defects were performed. Afterwards, the animals were randomly divided into three groups according to filler material: a blood clot group (BC), blood clot membrane group (BCM), and an injectable β-tricalcium phosphate group (HBS) cement group. After periods of 30 and 60 days, the animals were euthanized, the calvaria was isolated, and submitted to a decalcification process for later blades confection. Qualitative and quantitative analysis of the neoformed bone tissue were conducted, and histometric data were statistically analyzed. (4) Results: Sixty days post-surgery, the percentages of neoformed bone were 10.67 ± 5.57 in group BC, 16.71 ± 5.0 in group BCM, and 55.11 ± 13.20 in group HBS. The bone formation values in group HBS were significantly higher (p < 0.05) than in groups BC and BCM. (5) Conclusions: Based on these results, it can be concluded that injectable calcium phosphate cement is an osteoconductive material that can be used to fill bone cavities.

scholarly journals A facile one-stage treatment of critical bone defects using a calcium sulfate/hydroxyapatite biomaterial providing spatiotemporal delivery of bone morphogenic protein–2 and zoledronic acid

2020 ◽  
Vol 6 (48) ◽  
pp. eabc1779
Author(s):  
Deepak Bushan Raina ◽  
Lucas-Maximilian Matuszewski ◽  
Corina Vater ◽  
Julia Bolte ◽  
Hanna Isaksson ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Calcium Sulfate ◽  
Bone Defects ◽  
Bone Morphogenic Proteins ◽  
Femoral Defect ◽  
Defect Healing ◽  
Critical Defect ◽  
Delivery Platform ◽  
Critical Bone Defects ◽  
Bone Morphogenic Protein 2

Bone morphogenic proteins (BMPs) are the only true osteoinductive molecules. Despite being tremendously potent, their clinical use has been limited for reasons including supraphysiological doses, suboptimal delivery systems, and the pro-osteoclast effect of BMPs. Efforts to achieve spatially controlled bone formation using BMPs are being made. We demonstrate that a carrier consisting of a powder of calcium sulfate/hydroxyapatite (CaS/HA) mixed with bone active molecules provides an efficient drug delivery platform for critical femoral defect healing in rats. The bone-active molecules were composed of osteoinductive rhBMP-2 and the bisphosphonate, and zoledronic acid (ZA) was chosen to overcome BMP-2–induced bone resorption. It was demonstrated that delivery of rhBMP-2 was necessary for critical defect healing and restoration of mechanical properties, but codelivery of BMP-2 and ZA led to denser and stronger fracture calluses. Together, the CaS/HA biomaterial with rhBMP-2 and/or ZA can potentially be used as an off-the-shelf alternative to autograft bone.

scholarly journals Osteoconductive Properties of a Volume-Stable Collagen Matrix in Rat Calvaria Defects: A Pilot Study

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 732
Author(s):  
Karol Alí Apaza Alccayhuaman ◽  
Stefan Tangl ◽  
Stéphane Blouin ◽  
Markus A. Hartmann ◽  
Patrick Heimel ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Collagen Matrix ◽  
Microcomputed Tomography ◽  
Soft Tissue Augmentation ◽  
Backscattered Electron Imaging ◽  
Quantitative Backscattered Electron Imaging ◽  
Rat Calvaria ◽  
Backscattered Electron ◽  
Electron Imaging

Volume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat calvaria circular defects with a VSCM. After four weeks, histology, histomorphometry, quantitative backscattered electron imaging, and microcomputed tomography were performed. We report that the overall pattern of mineralization inside the VSCM was heterogeneous. Histology revealed, apart from the characteristic woven bone formation, areas of round-shaped hypertrophic chondrocyte-like cells surrounded by a mineralized extracellular matrix. Quantitative backscattered electron imaging confirmed the heterogenous mineralization occurring within the VSCM. Histomorphometry found new bone to be 0.7 mm2 (0.01 min; 2.4 max), similar to the chondrogenic mineralized extracellular matrix with 0.7 mm2 (0.0 min; 4.2 max). Microcomputed tomography showed the overall mineralized tissue in the defect to be 1.6 mm3 (min 0.0; max 13.3). These findings suggest that in a rat cranial defect, VSCM has a limited and heterogeneous capacity to support intramembranous bone formation but may allow the formation of bone via the endochondral route.

Clinical feasibility of fracture healing assessment through continuous monitoring of implant load

2020 ◽  
pp. 110188
Author(s):  
Manuela Ernst ◽  
Heiko Baumgartner ◽  
Stefan Döbele ◽  
Dankward Höntzsch ◽  
Tim Pohlemann ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Continuous Monitoring ◽  
Healing Assessment

Long-term pretreatment with alendronate inhibits calvarial defect healing in an osteoporotic rat model

2021 ◽  
Author(s):  
Chenggui Zhang ◽  
Junxiong Zhu ◽  
Jialin Jia ◽  
Zhiyuan Guan ◽  
Tiantong Sun ◽  
...  
Keyword(s):  
Rat Model ◽  
Calvarial Defect ◽  
Defect Healing

A simplified framework for prediction of sensor network coverage in real-time structural health monitoring of plate-like structures

2021 ◽  
pp. 147592172110332
Author(s):  
Mehrdad Ghyabi ◽  
Hamidreza Nemati ◽  
Ehsan Dehghan-Niri
Keyword(s):  
Finite Element ◽  
Sensor Network ◽  
Health Monitoring ◽  
Surface Crack ◽  
Piezoelectric Sensor ◽  
Network Coverage ◽  
Coverage Area ◽  
Fem Simulations ◽  
Network Geometry ◽  
Critical Defect

In this article, the coverage area prediction of piezoelectric sensor network for detecting a specific type of under-surface crack in plate-like structures is addressed. In particular, this article proposes a simplified framework to estimate the coverage of any given sensor network arrangement when a critical defect is known. Based on numerical results from finite element methods (FEM), a simplified framework to estimate coverage area of any given network arrangement is developed. Using such a simplified framework, one can avoid time-consuming procedure of evaluating numerous FEM models in estimating sensor network coverage. Back-scatter fields of partial cracks are estimated using a proposed function, whose parameters are estimated from the results of a limited number of FEM simulations. The proposed function efficiently predicts the back-scattered field of any combination of transmitters and receivers for a given crack geometry. Superposition is used to estimate the coverage area of an arbitrary piezoelectric (e.g., PZT) sensor network. It is shown that the coverage area of a sensor network depends on both sensor network geometry and defect properties (e.g., crack inclination) and it is not necessarily a linear function of the number of sensors. Furthermore, it is shown that the network arrangement has an important effect on the geometry of the coverage area. Experimental results of a network of 14 PZTs in two clusters confirm the accuracy of the method.

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