Wheels for Genus Identification and Presenting Diversity in Kingdom Fungi

The provision of rapid results from positive blood cultures is important for the clinical management of septicaemia. This study tested the accuracy of direct inoculation of biochemical tests from positive blood culture vials for the identification of members of the Enterobacteriaceae and Acinetobacter species. A hundred and eighty-one samples were included in the study, with 25 % subsequently excluded as a result of mixed colonial growth. The study method successfully identified 133 (98 %) isolates from 136 vials to genus level and was technically simple to perform, requiring an additional 3 min for the processing of each positive vial. The results of this study demonstrate that a direct inoculation method provides acceptable genus identification of Gram-negative bacilli in positive blood culture vials, with a potential saving of 24 h compared with traditional methods.

Download Full-text

Attraction of mosquitoes to domestic cats in a heartworm enzootic region

Journal of Feline Medicine and Surgery ◽

10.1016/j.jfms.2007.01.009 ◽

2007 ◽

Vol 9 (4) ◽

pp. 309-312 ◽

Cited By ~ 2

Author(s):

Luciana AM Gomes ◽

Maria Lucia Serrão ◽

Rosemere Duarte ◽

Alexandre Bendas ◽

Norma Labarthe

Keyword(s):

Blood Meal ◽

Dirofilaria Immitis ◽

Mammalian Species ◽

Diagnostic Efficiency ◽

Worldwide Distribution ◽

Heartworm Disease ◽

Heartworm Infection ◽

Low Prevalence ◽

Genus Identification ◽

Blood Meal Identification

Heartworm disease is caused by a mosquito-borne parasite that can affect many different mammalian species and has worldwide distribution. The agent, Dirofilaria immitis (Leidy 1856), infect mainly dogs but feline infection have been frequently reported in the last decade. Feline heartworm infection is difficult to detect, therefore, low reported prevalence could reflect true low prevalence or poor diagnostic efficiency. As mosquitoes are known to be attracted differently by different mammalian species, mosquitoes were collected from both a cattery and a contiguous home located in a canine heartworm enzootic area in Niterói, state of Rio de Janeiro, Brazil. For 14 months, mosquitoes were collected weekly for genus identification, speciation when possible, and for individual blood meal identification. Culex species mosquitoes were the most captured and those most frequently found with feline blood meal, followed by Aedes species that, although captured in lower numbers, also fed on feline blood. While Culex species mosquitoes have been reported as potential secondary heartworm vectors for dogs and primary vectors for cats, the present results suggest that Aedes species mosquitoes may also be involved in feline heartworm transmission in a larger proportion than previously thought.

Download Full-text

Ectomycorrhizal Fungi on South Kalimantan Serpentine Soil

Jurnal Mikologi Indonesia ◽

10.46638/jmi.v4i1.71 ◽

2020 ◽

Vol 4 (1) ◽

Author(s):

Rudy Hermawan ◽

Witiyasti Imaningsih ◽

Badruzsaufari Badruzsaufari

Keyword(s):

Heavy Metals ◽

Ectomycorrhizal Fungi ◽

Morphological Characters ◽

Ectomycorrhizal Fungus ◽

Serpentine Soil ◽

Fruiting Bodies ◽

Genus Identification ◽

Manganese Chromium ◽

Cobalt And Nickel

Serpentine soil contains highly heavy metals, such as manganese, chromium, cobalt, and nickel,which could bean inappropriate growthmediaofmostplants. Someplants thatfound able to grow optimally on South Kalimantan serpentine soil have been known to do association with ectomycorrhizal fungi. This research aimed to obtain and characterize mushrooms assumed as ectomycorrhizal fungi indigenous South Kalimantan serpentine soil. This study used field exploration of fungal fruiting bodies and identified the genus based on morphological characters of fruiting bodies such as shape, size, and ornamentation, which are unique for the genus identification, then compared the characteristics on mushroomexpert.com. The mushrooms were also confirmed of genera assumed as ectomycorrhizal fungi based on mycorrhizas.info. Seven fruiting bodies were obtained and classified as Cantharellus (Ct), Chlorophyllum (Ch1 and Ch2), Lycoperdon (Ly), Ramaria (Rm1 and Rm2), and Thelephora (Tp). The results showed that all of those fruiting bodies belong to Basidiomycetes. There were 4 genera of Cantharellus, Lycoperdon, Ramaria, and Thelephora, assumed as ectomycorrhizal fungi. But Chlorophyllum genus was never reported as ectomycorrhizal fungus

Download Full-text

Comparison of four methods for identification of bifidobacteria to the genus level

Czech Journal of Food Sciences ◽

10.17221/3528-cjfs ◽

2011 ◽

Vol 20 (No. 5) ◽

pp. 171-174 ◽

Cited By ~ 7

Author(s):

E. Vlková ◽

J. Medková ◽

V. Rada

Keyword(s):

Intestinal Tract ◽

Fermented Milk ◽

Specific Primers ◽

Laboratory Equipment ◽

Milk Products ◽

Genus Level ◽

Original Procedure ◽

Triton X ◽

Genus Identification ◽

Fermented Milk Products

The identification of bifidobacteria to the genus level is important for the differentiation of these bacteria from other bacteria occurring in the animal and human intestine. The detection of fructose-6-phosphate phosphoketolase (F6PPK-test) is used traditionally for the identification of Bifidobacterium sp. The original procedure is time consuming and therefore it was modified several times recently. The aim of the present work was to compare the following methods for the genus identification of bifidobacteria: F6PPK-test, F6PPK-test modified by the addition of triton X-100, F6PPK-test modified by the addition of cetridium bromide (F6PPK-CTAB-test), and PCR using genus specific primers. Bifidobacteria isolated from fermented milk products (3 strains), human faeces (6 strains), and animal intestinal tract (2 strains) were tested. All the methods tested proved to be reliable tests for the genus identification of bifidobacteria. The F6PPK-CTAB-test gave the best results. This procedure is quick and does not require any special laboratory equipment.  

Download Full-text

Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2015-0066 ◽

2015 ◽

Vol 18 (3) ◽

pp. 507-514 ◽

Cited By ~ 8

Author(s):

A. Kandričáková ◽

A. Lauková ◽

V. Strompfová

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Species Identification ◽

Virulence Factor ◽

Identification System ◽

Limited Information ◽

Biochemical Tests ◽

Average Value ◽

Genus Identification ◽

Virulence Factor Genes

AbstractWith an increasing number of pheasants as gamebirds being reared each year, these species are becoming a more prominent part of the workload of many veterinary practices. Only limited information can be found concerning the microflora of common pheasants. A significant part of the obligate microflora consists of lactic acid bacteria, including enterococci. In this study, faeces were sampled from 60 pheasants aged 16-17 weeks. Enterococcal counts reached 5.48±1.9 (log10) CFU/g. Strains (17) were taxonomically classified to the genus Enterococcus using the Maldi-Tof identification system; they were allotted to the speciesE. hirae(58.8%),E. faecium(23.5%) andE. faecalis(17.7%) by highly probable species identification or by secure genus identification/probable species identication. Species allocation was also confirmed using conventional biochemical tests. Most strains formed β-hemolysis. Gelatinase active phenotype was found in threeE. faecalisstrains. Enterococci were β-glucuronidase negative, mostly trypsin negative with slight or moderate production of α-chymotrypsin. EH52b and EF42 strains possessed the highest potential for pathogenicity. Average value of lactic acid was 1.78±0.33 mmo/L. Most strains were tetracycline resistant (82.4%). PolyresistantE. faecalisstrains with positive gelatinase phenotype and possessing virulence factor genes confirmed using PCR (gelE, efaAfs,ccf cob, cpd) were sensitive to enterocins (activity 1600-25 600 AU/mL).

Download Full-text