A brief commentary on ‘Response letter to “What should be taken into account during study on immunoregulatory effects of helminths: a critical analyzing on downregulation of immune responses in asthmatic patients by ES products of
            Marshallagia marshalli”
            ’

Background: The current study was conducted to investigate the antigenic effect of Marshallagia marshalli on the treatment of asthma by measuring the secreted inhibitory cytokine. Methods: Case patients and controls were selected from clinics in Mashhad, Khorasan Razavi Province, Northeastern Iran in 2017-18. In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from 15 patients with asthma and 10 healthy controls and were cultured. PBMCs were then converted to tolerogenic DCs through exposure to GM-CSF, IL-4 and M. marshalli antigen. Then, tolerogenic DCs were exposed to autologous T cells for five days and finally, the level of secreted TGF-β1 was measured. Results: The mean TGF-β1 level in the control and control groups was 210.2 ± 8.2 and 225.4 ± 6.1 pq/ml, respectively. The results showed that TGF-β1 levels in both groups significantly increased in both groups (P<0.001). In addition, TGF-β1 levels in the case group were significantly higher than the control group (P<0.001). Conclusion: M. marshalli antigen increase the level of TGF-β1 and can create antigen-bearing dendritic cells and shift T lymphocytes to the regulatory type. This parasite can be used in dendritic cell therapy to control allergic diseases.

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Somatic extracts of Marshallagia marshalli downregulate the Th2 associated immune responses in ovalbumin-induced airway inflammation in BALB/c mice

Parasites & Vectors ◽

10.1186/s13071-017-2159-8 ◽

2017 ◽

Vol 10 (1) ◽

Cited By ~ 4

Author(s):

Sima Parande Shirvan ◽

Azadeh Ebrahimby ◽

Arezoo Dousty ◽

Mohsen Maleki ◽

Ahmadreza Movassaghi ◽

...

Keyword(s):

Immune Responses ◽

Airway Inflammation ◽

Marshallagia Marshalli

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Role of TGF-β in anti-rhinovirus immune responses in asthmatic patients

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2016.10.049 ◽

2017 ◽

Vol 140 (1) ◽

pp. 283-286.e10 ◽

Cited By ~ 6

Author(s):

Carina Bielor ◽

Nina Sopel ◽

Anja Maier ◽

Ashley Blau ◽

Himanshu Sharma ◽

...

Keyword(s):

Immune Responses ◽

Asthmatic Patients

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What should be taken into account during study on immunoregulatory effects of helminths: a critical analyzing on “Downregulation of immune responses in asthmatic patients by ES products ofMarshallagia marshalli”

The Clinical Respiratory Journal ◽

10.1111/crj.12350 ◽

2015 ◽

Vol 11 (1) ◽

pp. 130-131 ◽

Cited By ~ 3

Author(s):

Amin Zakeri ◽

Alireza Haghparast

Keyword(s):

Immune Responses ◽

Asthmatic Patients

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Sequential degranulation of eosinophils induced by antigen or autocoids in allergic humans

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143390 ◽

1986 ◽

Vol 44 ◽

pp. 354-355

Author(s):

Kate W. Sjoerdsma ◽

W. James Metzger

Keyword(s):

Allergic Rhinitis ◽

Bronchoalveolar Lavage ◽

Allergic Asthma ◽

Skin Test ◽

Positive Skin Test ◽

Positive Skin ◽

Human Eosinophil ◽

Asthmatic Patients ◽

Allergic Asthmatic ◽

History Of

Eosinophils are important to the pathogenesis of allergic asthma, and are increased in bronchoalveolar lavage within four hours after bronchoprovocation of allergic asthmatic patients, and remain significantly increased up to 24 hours later. While the components of human eosinophil granules have been recently isolated and purified, the mechanisms of degranulation have yet to be elucidated.We obtained blood from two volunteers who had a history of allergic rhinitis and asthma and a positive skin test (5x5mm wheal) to Alternaria and Ragweed. Eosinophils were obtained using a modification of the method described by Roberts and Gallin.

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