scholarly journals Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jong-Hyeok Jung ◽  
Hyun Jung Hong ◽  
Aziz Gharderpour ◽  
Jae Young Cho ◽  
Bum-Seo Baek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Renal Scarring ◽  
Long Term Effects ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Serum Crp

Abstract Urinary tract infection (UTI) is one of the most common bacterial infections in infants less than age 1 year. UTIs frequently recur and result in long-term effects include sepsis and renal scarring. Uropathogenic Escherichia coli (UPEC), the most prevalent organism found in UTIs, can cause host inflammation via various virulence factors including hemolysin and cytotoxic necrotizing factors by inducing inflammatory cytokines such as interleukin (IL)-1β. However, the ability of each UPEC organism to induce IL-1β production may differ by strain. Furthermore, the correlation between differential IL-1β induction and its relevance in pathology has not been well studied. In this study, we isolated UPEC from children under age 24 months and infected bone-marrow derived macrophages with the isolates to investigate secretion of IL-1β. We found that children with higher concentrations of C-reactive protein (CRP) were more likely to harbor phylotype B2 UPEC strains that induced more IL-1β production than phylotype D. We also observed a significant correlation between serum CRP level and in vitro IL-1β induction by phylotype B2 UPEC bacteria. Our results highlight the diversity of UPEC in terms of IL-1β induction capacity in macrophages and suggest a potential pathogenic role in UTIs by inducing inflammation in infants.

scholarly journals Long-term effects of the proline-rich antimicrobial peptide Oncocin112 on the Escherichia coli translation machinery

2020 ◽  
Vol 295 (38) ◽  
pp. 13314-13325
Author(s):  
Yanyu Zhu ◽  
James C. Weisshaar ◽  
Mainak Mustafi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Peptides ◽  
Binding Site ◽  
Elongation Factor ◽  
Microscopy Study ◽  
Single Step ◽  
Long Term Effects ◽  
Bacterial Membranes

Proline-rich antimicrobial peptides (PrAMPs) are cationic antimicrobial peptides unusual for their ability to penetrate bacterial membranes and kill cells without causing membrane permeabilization. Structural studies show that many such PrAMPs bind deep in the peptide exit channel of the ribosome, near the peptidyl transfer center. Biochemical studies of the particular synthetic PrAMP oncocin112 (Onc112) suggest that on reaching the cytoplasm, the peptide occupies its binding site prior to the transition from initiation to the elongation phase of translation, thus blocking further initiation events. We present a superresolution fluorescence microscopy study of the long-term effects of Onc112 on ribosome, elongation factor-Tu (EF-Tu), and DNA spatial distributions and diffusive properties in intact Escherichia coli cells. The new data corroborate earlier mechanistic inferences from studies in vitro. Comparisons with the diffusive behavior induced by the ribosome-binding antibiotics chloramphenicol and kasugamycin show how the specific location of each agent's ribosomal binding site affects the long-term distribution of ribosomal species between 30S and 50S subunits versus 70S polysomes. Analysis of the single-step displacements from ribosome and EF-Tu diffusive trajectories before and after Onc112 treatment suggests that the act of codon testing of noncognate ternary complexes (TCs) at the ribosomal A-site enhances the dissociation rate of such TCs from their L7/L12 tethers. Testing and rejection of noncognate TCs on a sub-ms timescale is essential to enable incorporation of the rare cognate amino acids into the growing peptide chain at a rate of ∼20 aa/s.

scholarly journals Long-Term Effects of Pravastatin on Plasma Concentration of C-reactive Protein

Circulation ◽  
1999 ◽  
Vol 100 (3) ◽  
pp. 230-235 ◽  
Author(s):  
Paul M. Ridker ◽  
Nader Rifai ◽  
Marc A. Pfeffer ◽  
Frank Sacks ◽  
Eugene Braunwald
Keyword(s):  
Plasma Concentration ◽  
Long Term Effects ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals C-reactive protein in dogs with suspected bacterial diskospondylitis: 16 cases (2010–2019)

2020 ◽  
Vol 7 (1) ◽  
pp. e000386
Author(s):  
George Nye ◽  
Francois-Xavier Liebel ◽  
Tom Harcourt-Brown
Keyword(s):  
Back Pain ◽  
Antimicrobial Treatment ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Inflammatory Conditions ◽  
Immune Mediated ◽  
A Prospective Study ◽  
Serum Crp

ObjectivesC-reactive protein (CRP) is an acute phase protein used in multiple canine inflammatory conditions including steroid responsive meningitis-arteritis, immune-mediated polyarthritis and bronchopneumonia. The aim of this study was to assess whether serum CRP is elevated in cases of diskospondylitis.MethodsMedical records from 2010 to 2019 were searched to identify dogs diagnosed with diskospondylitis based on findings consistent on CT or MRI and with CRP tested.ResultsA total of 16 dogs met the inclusion criteria. All cases had back pain. Fourteen cases had elevated CRP, with a median value of 100.7 mg/l (reference range for CRP values: 0–10 mg/l), 12 were pyrexic and six had leucocytosis. The two dogs with normal CRP were normothermic and did not have leucocytosis. CRP was measured four to six weeks into antimicrobial treatment in eight of 14 dogs and was normal in all cases. One dog developed a suspected bacterial empyema diagnosed on MRI; this occurred two weeks after antibiotic treatment was discontinued based on a normal CRP level at follow-up.ConclusionsSerum CRP is elevated in cases of diskospondylitis and may be clinically more useful to screen dogs with back pain than pyrexia or leucocytosis alone. Further long-term clinical evaluation in a prospective study is needed to assess its use as a treatment monitoring tool and in decision making.

Long-Term Stability of High Sensitivity C Reactive Protein Levels in Healthy Adults

2007 ◽  
Vol 16 ◽  
pp. S68
Author(s):  
B.M. McQuillan ◽  
E. Rossi ◽  
J. Hung ◽  
P.L. Thompson ◽  
J.P. Beilby
Keyword(s):  
High Sensitivity ◽  
Healthy Adults ◽  
C Reactive Protein ◽  
Term Stability ◽  
Protein Levels ◽  
Long Term Stability ◽  
Reactive Protein

Does atorvastatin influence serum C-reactive protein levels in patients on long-term hemodialysis?

2004 ◽  
Vol 43 (3) ◽  
pp. 471-478 ◽  
Author(s):  
Luigi Vernaglione ◽  
Claudio Cristofano ◽  
Pietro Muscogiuri ◽  
Stefano Chimienti
Keyword(s):  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein
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