In vivo anti‐ageing activity of cream containing niosomes loaded with purple glutinous rice ( Oryza sativa Linn.) extract

2020 ◽  
Vol 42 (6) ◽  
pp. 622-631 ◽  
Author(s):  
J. Manosroi ◽  
C. Chankhampan ◽  
W. Kitdamrongtham ◽  
J. Zhang ◽  
M. Abe ◽  
...  
Keyword(s):  
Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 685
Author(s):  
Enerand Mackon ◽  
Yafei Ma ◽  
Guibeline Charlie Jeazet Dongho Epse Mackon ◽  
Qiufeng Li ◽  
Qiong Zhou ◽  
...  

Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis. However, there is still no scientific evidence supporting the existence of compartmentalization and trafficking of anthocyanin inside the cells. In the current study, we took advantage of autofluorescence with anthocyanin’s unique excitation/emission properties to elucidate the subcellular localization of anthocyanin and report on the in planta characterization of anthocyanin prevacuolar vesicles (APV) and anthocyanic vacuolar inclusion (AVI) structure. Protoplasts were isolated from the stigma of black and brown rice and imaging using a confocal microscope. Our result showed the fluorescence displaying magenta color in purple stigma and no fluorescence in white stigma when excitation was provided by a helium–neon 552 nm and emission long pass 610–670 nm laser. The fluorescence was distributed throughout the cell, mainly in the central vacuole. Fluorescent images revealed two pools of anthocyanin inside the cells. The diffuse pools were largely found inside the vacuole lumen, while the body structures could be observed mostly inside the cytoplasm (APV) and slightly inside the vacuole (AVI) with different shapes, sizes, and color intensity. Based on their sizes, AVI could be grouped into small (Ф < 0.5 um), middle (Ф between 0.5 and 1 um), and large size (Ф > 1 um). Together, these results provided evidence about the sequestration and trafficking of anthocyanin from the cytoplasm to the central vacuole and the existence of different transport mechanisms of anthocyanin. Our results suggest that stigma cells are an excellent system for in vivo studying of anthocyanin in rice and provide a good foundation for understanding anthocyanin metabolism in plants, sequestration, and trafficking in black rice.


2020 ◽  
Vol 57 (8) ◽  
pp. 3157-3163
Author(s):  
Rakrudee Sarnthima ◽  
Saranyu Khammuang ◽  
Anupong Joompang

2012 ◽  
Vol 17 (1) ◽  
pp. 302-305 ◽  
Author(s):  
Nattaya Lourith ◽  
Mayuree Kanlayavattanakul
Keyword(s):  

2012 ◽  
Vol 16 (1) ◽  
pp. 222-228 ◽  
Author(s):  
Aranya Manosroi ◽  
Warintorn Ruksiriwanich ◽  
Worapaka Manosroi ◽  
Masahiko Abe ◽  
Jiradej Manosroi

2010 ◽  
Vol 122 (1) ◽  
pp. 63-76 ◽  
Author(s):  
Chang-Jie Yan ◽  
Zhi-Xi Tian ◽  
Yu-Wei Fang ◽  
Ya-Chun Yang ◽  
Jian Li ◽  
...  

2019 ◽  
Vol 20 (2) ◽  
pp. 6-16
Author(s):  
Sri Benti Etika ◽  
Iryani Iryani

This study aims to isolate and characterize the isolated flavonoid compounds from glutinous rice (Oryza Sativa L. Var Glutinosa). The method that used was maserasi with methanol solvent, multilevel fractionation with n-hexane and ethyl acetate. The separation of chemical components using column chromatography and tested its purity by melting point test and TLC. Characterization of isolated flavonoids using color reagents (H2SO4, NaOH, Mg-HCl), KKt-2A, UV-Vis and FT-IR. The powder (amorphous) flavonoid isolation results have a melting point of 236.5-237.8 oC. Flavonoid powder added H2SO4 produces a yellow color, with NaOH producing a violet color, and Mg-HCl produces a red color. KKt-2A with a BAA developer has Rf 0.49 and 15% acetic acid having Rf 0.09. The UV-Vis spectrum shows maximum absorption at 532 nm wavelength. The results of powder analysis (amorphous) flavonoid using FT-IR showed absorption at wave number 3.330,40 cm-1, 2838,96 cm-1, 1,656,23 cm-1, 1,421,85 cm-1, 1,112,25 cm-1, 1,019,27 cm-1 and 697,76 cm-1. According to those data it can be concluded that flavonoids that isolated have the form of powder (amorphous) which belong to anthocyanin group that have o-diOH on ring B and have a conjugated double bond.Keywords- Black Sticky Rice, Flavonoid, Fractionation, FT-IR, Maserasi, UV-Vis.


2008 ◽  
Vol 7 (2) ◽  
pp. 140-148 ◽  
Author(s):  
C. Bounphanou ◽  
P. Jaisil ◽  
K.L. McNally ◽  
J. Sanitchon ◽  
N.R. Sackville Hamilton

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