Hydrolysis degree, peptide profile and phenylalanine removal from whey protein concentrate hydrolysates obtained by various proteases

2012 ◽  
Vol 48 (3) ◽  
pp. 588-595 ◽  
Author(s):  
Marialice P. C. Silvestre ◽  
Maitê C. da Silva ◽  
Mariana W. S. de Souza ◽  
Viviane D. M. Silva ◽  
Marcos J. B. de Aguiar ◽  
...  
Keyword(s):  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Peptide Profile ◽  
Hydrolysis Degree

scholarly journals Action of protease from Aspergillus sojae and pancreatin in the hydrolysis of whey protein concentrate

2013 ◽  
Vol 3 (1) ◽  
pp. 19
Author(s):  
Marialice Pinto Coelho Silvestre ◽  
Harriman Aley Morais ◽  
Mauro Ramalho Silva ◽  
Viviane Dias Medeiros Silva ◽  
Mariana Wanessa Santana de Souza
Keyword(s):  
Amino Acids ◽  
Whey Protein ◽  
Free Amino Acids ◽  
Free Amino ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Peptide Profile ◽  
Aspergillus Sojae ◽  
Enzyme Substrate ◽  
Substrate Ratio

<p>The preparation of hydrolysates from whey protein concentrate with high contents of di- and tripeptides and free amino acids, and reduced amount of large peptides, was the objective of this study. The actions of a protease from Aspergillus sojae and a pancreatin at six enzyme:substrate ratios, pH 7.0, at 50 &deg;C for 5 h were evaluated. The size-exclusion-high performance liquid chromatography and the method of the Correct Fraction Area were used for the fractionation and quantification of peptides and free amino acids in the hydrolysates. It was observed that the type of enzyme and the enzyme: substrate ratio affected significantly the peptide profile of the hydrolysates. The chromatograms obtained by the action of two enzymes were different and varied according to the specificity or enzymatic activity. The best peptide profile was found for the hydrolysate obtained using pancreatin at an enzyme: substrate ratio of 8:100 that showed the smallest amount of large peptides (41.13%), and the greatest di- and tripeptide (6.46%) and free amino acid (8.22%) contents.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v3i1.69</p><br />

Correlation between the Degree of Hydrolysis and the Peptide Profile of Whey Protein Concentrate Hydrolysates: Effect of the Enzyme Type and Reaction Time

2012 ◽  
Vol 8 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Harriman A. Morais ◽  
Marialice P.C. Silve ◽  
Viviane D.M. Silva ◽  
Mauro R. Silva ◽  
Ana Cristina S ◽  
...  
Keyword(s):  
Reaction Time ◽  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Degree Of Hydrolysis ◽  
Protein Concentrate ◽  
Peptide Profile

Enzymatic hydrolysis of whey protein concentrate: effect of enzyme type and enzyme:substrate ratio on peptide profile

2013 ◽  
Vol 52 (1) ◽  
pp. 201-210 ◽  
Author(s):  
Harriman Aley Morais ◽  
Marialice Pinto Coelho Silvestre ◽  
Mauro Ramalho Silva ◽  
Viviane Dias Medeiros Silva ◽  
Marina Andrade Batista ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Peptide Profile ◽  
Hydrolysis Of

Use of Different Proteases to Obtain Whey Protein Concentrate Hydrolysates with Inhibitory Activity toward Angiotensin-Converting Enzyme

2013 ◽  
Vol 38 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Harriman A. Morais ◽  
Marialice P.C. Silvestre ◽  
Larissa L. Amorin ◽  
Viviane D.M. Silva ◽  
Mauro R. Silva ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Whey Protein ◽  
Inhibitory Activity ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Converting Enzyme

scholarly journals Effects of incorporation of whey protein concentrate on physicochemical, texture, and microbial evaluation of developed cookies

2015 ◽  
Vol 1 (1) ◽  
pp. 1092406 ◽  
Author(s):  
Safa Hamid Wani ◽  
Amir Gull ◽  
Farhana Allaie ◽  
Tariq Ahmad Safapuri ◽  
Fatih Yildiz
Keyword(s):  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

Preparation of iron/zinc bound whey protein concentrate complexes and their stability

LWT ◽  
2016 ◽  
Vol 66 ◽  
pp. 514-522 ◽  
Author(s):  
B.G. Shilpashree ◽  
Sumit Arora ◽  
Vivek Sharma
Keyword(s):  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Protein Concentrate
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