The association of −330 interleukin-2 gene polymorphism and HLA-DR15 allele in Iranian patients with multiple sclerosis

Multiple sclerosis (MS) is a chronic disease characterized by degeneration of the central nervous system (CNS). High levels of Neurofilament heavy chain (NEFH) in cerebrospinal fluid (CSF) is associated with MS. 40 MS patients and 40 controls genotyped by polymerase chain reaction (PCR) and Sanger sequencing. Genotypic and allelic distributions were compared between cases and controls. Fisher test was used to estimate the risk of MS associated with genotypes. We showed that NEFH, 1084-244G>A gene polymorphism, has no significant association with the susceptibility or severity of MS in Iranian patients (P = 0.737). Further prospective studies are required for confirmation.

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Correlation of interleukin-2 and uric acid plasma con-centration with multiple sclerosis in Iranian patients

Journal of Biology and Today s World ◽

10.15412/j.jbtw.01020402 ◽

2013 ◽

Vol 2 (4) ◽

Cited By ~ 1

Author(s):

A. Sayad ◽

A. Allameh ◽

Y. Mohseni

Keyword(s):

Multiple Sclerosis ◽

Uric Acid ◽

Interleukin 2 ◽

Iranian Patients

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Association between single nucleotide polymorphisms rs12722489 and multiple sclerosis in Iranian patients with multiple sclerosis

Iranian Journal of Neurology ◽

10.18502/ijnl.v19i1.3287 ◽

2020 ◽

Author(s):

Habib Ahmadi ◽

Vahid Reza Yassaee ◽

Reza Mirfakhraie ◽

Feyzollah Hashemi-Gorji

Keyword(s):

Multiple Sclerosis ◽

Single Nucleotide Polymorphisms ◽

Interleukin 2 ◽

European Population ◽

Control Group ◽

Amplification Refractory Mutation System ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Mutation System ◽

Iranian Patients

Abstract Background: Multiple sclerosis (MS) is a complex incurable neurodegenerative disease featuring demyelination of neurons, resulting in impairment of neuron impulses. Recently, an association of two single nucleotide polymorphisms (SNP) (rs2104286 and rs12722489) in interleukin 2 receptor subunit alpha (IL2RA) gene was found to be a risk factor of MS in white European population. Therefore, we performed a study to investigate the contribution of these two intronic variations in Iranian patients with MS. Methods: We determined the genotypes of rs2104286 and rs12722489 in patients with MS (n = 100) and in the control group (n = 111). The SNPs were genotyped using tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) for both of SNPs. Statistical analysis was performed by SPSS software. Also, odds ratios (ORs) and 95% confidence interval (CI) were calculated. Results: Logistic regression revealed that various genotypes of rs12722489, regarding sex-adjusted effect, yielded meaningful association with MS risk in Iranian patients (OR = 2.67, 95% CI: 1.03-6.90). However, no association was obtained for rs2104286 and rs12722489 with MS. Conclusion: The results confirmed partially the reports in white European population performed recently. However, further investigation in larger scale is necessary to validate our study.

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The Association of −330 Interleukin-2 Gene Polymorphism with Its Plasma Concentration in Iranian Multiple Sclerosis Patients

Scientifica ◽

10.1155/2014/724653 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 3

Author(s):

Arezou Sayad ◽

Abolfazl Movafagh

Keyword(s):

Multiple Sclerosis ◽

Plasma Concentration ◽

Gene Polymorphism ◽

Plasma Levels ◽

Demyelinating Disease ◽

Interleukin 2 ◽

Control Group ◽

Normal Individuals ◽

The Central Nervous System ◽

Multiple Sclerosis Patients

Multiple sclerosis (MS) is a chronic neuroinflammatory demyelinating disease of the central nervous system. The cytokine genes are involved in autoimmune diseases such as MS. In this study, we report the influence of −330 interleukin-2 (IL2) gene polymorphism on its plasma levels in a group of Iranian MS patients. In this study 100 MS patients and 100 ethnically, age, and sex matched healthy controls were selected from Medical Genetics Department of Sarem Women Hospital. Blood samples of all individuals were collected in EDTA tubes. The restriction fragment length polymorphism PCR (RFLP) method was applied to determine various alleles and genotypes in these individuals. Plasma concentration of IL2 was measured in all the samples using human IL2 kit. The frequency of −330 T/T IL2 genotype was higher in MS patients compared to normal individuals. Accordingly, the plasma levels of IL2 were significantly higher (P<0.0001) in patients when compared to the control group. In conclusion, in case of MS patients the −330 T/T IL2 genotype is associated with higher plasma levels of IL2.

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