Effect of cephapirin on tubular reabsorption of amino acids, uric acid and beta 2-microglobulin in man.

The persistence of high concentrations of beta-2-microglobulin (β2M) in the blood of patients with acute renal failure leads to the development of the dialysis-related amyloidosis. This disease manifests in the deposition of amyloid fibrils formed from the various forms of β2M in the tissues and biological fluids of patients. In this paper, the amyloid fibrils formed from the full-length β2M (β2m) and its variants that lack the 6 and 10 N-terminal amino acids of the protein polypeptide chain (ΔN6β2m and ΔN10β2m, respectively) were probed by using the fluorescent dye thioflavin T (ThT). For this aim, the tested solutions were prepared via the equilibrium microdialysis approach. Spectroscopic analysis of the obtained samples allowed us to detect one binding mode (type) of ThT interaction with all the studied variants of β2M amyloid fibrils with affinity ~104 M−1. This interaction can be explained by the dye molecules incorporation into the grooves that were formed by the amino acids side chains of amyloid protofibrils along the long axis of the fibrils. The decrease in the affinity and stoichiometry of the dye interaction with β2M fibrils, as well as in the fluorescence quantum yield and lifetime of the bound dye upon the shortening of the protein amino acid sequence were shown. The observed differences in the ThT-β2M fibrils binding parameters and characteristics of the bound dye allowed to prove not only the difference of the ΔN10β2m fibrils from other β2M fibrils (that can be detected visually, for example, by transmission electron microscopy (TEM), but also the differences between β2m and ΔN6β2m fibrils (that can not be unequivocally confirmed by other approaches). These results prove an essential role of N-terminal amino acids of the protein in the formation of the β2M amyloid fibrils. Information about amyloidogenic protein sequences can be claimed in the development of ways to inhibit β2M fibrillogenesis for the treatment of dialysis-related amyloidosis.

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PENGARUH EKSTRAK ETANOL DAUN KEMANGI (Ocimum bacilicum L.) TERHADAP KADAR β2 MIKROGLOBULIN, ASAM URAT DAN GAMBARAN HISTOLOGI GINJAL PADA TIKUS PUTIH GALUR WISTAR (Rattus novergicus strain Wistar) YANG DIINDUKSI MONOSODIUM GLUTAMAT

Herb-Medicine Journal ◽

10.30595/hmj.v3i1.6638 ◽

2020 ◽

Vol 3 (1) ◽

pp. 18

Author(s):

Paramita Septianawati ◽

Hernayanti Hernayanti ◽

Gratiana Ekaningsih W

Keyword(s):

Uric Acid ◽

Treatment Group ◽

Leaf Extract ◽

Kidney Tissue ◽

Blood Analysis ◽

Control Group ◽

Animal Group ◽

Wistar Strain ◽

Beta 2 ◽

Beta 2 Microglobulin

AbstractBasil leaves (Ocimum basilicum) is one of the traditional medicinal plants that is used as medicine.This study aims to determine the effect of giving basil leaves on levels of Beta 2 microglobulin, uric acid and kidney histology in white wistar-induced rats in MSG and determine the effective dose of basil leaves in an effort to improve kidney function.This study used 25 male Wistar strain rats with body weight 200-230 g. The test animal group was divided into five groups based on the stage of treatment. The first treatment group was a control group consisting of five mice that were not given basil leaf extract or MSG, the second treatment group was the group that was only given MSG without being given basil leaf extract, the third treatment group was the group given MSG followed by basil leaf extract dose 87, 5 mg / kgBB, the fourth treatment group was the group that was given MSG followed by the administration of basil leaf extract with a dose of 175 mg / kgBW, and the fifth treatment group was the group that was given MSG followed by the administration of basil leaf extract at a dose of 350 mg / kgBB. At the end of the treatment, rats were drawn for blood analysis of B2M levels and uric acid levels and kidney tissue uptake, as well as analyzing kidney tissue histology.The results showed that there were differences between Beta 2 microglobulin, uric acid and kidney histopathology of rats receiving basil leaf extract compared with MSG group without basil leaf extract. This study concludes that consuming basil leaf extract can reduce levels of B2M and uric acid and improve kidney histology induced by MSG.

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MO208ASSOCIATION OF SERUM BETA-2 MICROGLOBULIN LEVEL WITH DIFFERENT CARDIAC AND METABOLIC RISK FACTORS IN CKD PATIENTS

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfab092.0086 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

Nazia Arfin Siddiqui ◽

M Sahadat Hossain ◽

Babrul Alam ◽

S R Chaudhury ◽

Maa Chowdhury ◽

...

Keyword(s):

Risk Factors ◽

Chronic Kidney Disease ◽

Uric Acid ◽

Kidney Disease ◽

Serum Creatinine ◽

Serum Phosphate ◽

Metabolic Risk Factors ◽

Metabolic Risk ◽

Beta 2 ◽

Beta 2 Microglobulin

Abstract Background and Aims Serum β M is a middle molecule uremic toxin that accumulates in serum and deposits at various tissues in chronic kidney disease (CKD), especially more in dialysis patients. The βâ M is generally considered as a predictor of cardiovascular morbidity and mortality and this is more investigated in dialysis group. However its relationship with several cardiac and metabolic risk factors in all stages of CKD is still under evaluation. This study was undertaken to evaluate the association of plasma βâ M level in different stages of chronic kidney disease patients with different cardiac, renal and metabolic risk factors that can predict future cardiovascular events Method This cross-sectional study was conducted by selecting consecutive 132 CKD subjects of stages 1-5D including both patients not requiring dialysis and those on maintenance hemodialysis. Their demographic, clinical and laboratory data were recorded in a data sheet. Fasting blood samples in dialysis non requiring subjects and predialysis samples in hemodialysis group (G5D) were taken for testin in laboratory for CBC, serum βâ M, hCRP, iPTH, lipid profile, creatinine, uric acid and serum albumin as cardiac, renal and metabolic risk markers. Urine sample was taken from predialysis patients for chemical test and ACR. The CKD staging were done by MDRD criteria. Additional 25 no CKD subject was taken as healthy referents. Results Primarily Beta-2 microglobulin was higher in CKD patients than in healthy group (13.53 ± 14.74 vs. 1.81 ± 0.47, mg/l; p<.001). The levels were gradually rising with the advancing stages of CKD (G1&2-3.46 ± 2.39, G3-3.66 ± 1.08, G4-6.51 ± 2.20, G5-11.43 ± 2.98 and G5D-41.79 ± 8.58, mg/l). A Beta-2 microglobulin cut-off of >7.7 vs. < 7.7 mg/l showed significantly increased Systolic BP (136 ±22 vs. 123 ± 22, mmHg), diastolic BP (80 ± 12 vs. 75 ± 8.96, mmHg),( p<0.01); CRP (6.83 ± 6.03 vs. 4.39 ± 5.35, mg/l)( p<0.007); serum phosphate (4.84 ± 1.79 vs. 3.85 ±.92,mg/dl)( p<0.001); uric acid (5.89 ± 1.41 vs. 5.01 ± 1.57,mg/dl)( p<0.01); TG (189± 103 vs. 155 ± 88, mg/dl),( p<0.04); and PTH (239.83 ± 186.50 vs. 90.52 ± 81.77, pg/ml), ( p<0.001) indicating higher cardio metabolic risks in higher group. Similarly renal parameters were also more altered in high Beta-2 microalbumin group for serum creatinine (6.89 ± 3.54 vs. 1.58 ± .81, mg/dl) (p<0.001) and ACR (824 ± 917 vs. 320 ± 753, mg/g),( p<0.001). B-2 microglobulin also positively correlated with systolic blood pressure (r=.295, p<.001), serum creatinine (r=.879, p<.001), serum phosphate (r=.175, p =.047), serum iPTH (r=.403, p<.001) , hCRP ( r=.193, p =.050) , Triglycerides (r=.196, p =.023) and urine ACR in CKD patients. Conclusion Beta-2 microglobulin level was significantly higher in CKD with an increasing pattern towards advancing stages. The higher levels positively correlated with cardio renal and metabolic risk factors. Hence measuring Beta-2 microglobulin regularly can help to take preventive measures early to manage patients at risk.

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IgM interference in uric acid and beta-2-microglobulin: A case report

Clinica Chimica Acta ◽

10.1016/j.cca.2019.03.459 ◽

2019 ◽

Vol 493 ◽

pp. S221-S222

Author(s):

S. Pico ◽

M. Bernal ◽

A. Munoz ◽

S. Carrasco ◽

A. Criado ◽

...

Keyword(s):

Uric Acid ◽

Case Report ◽

Beta 2 ◽

Beta 2 Microglobulin

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Development of human renal function: reference intervals for 10 biochemical markers in fetal urine

Clinical Chemistry ◽

10.1093/clinchem/42.11.1855 ◽

1996 ◽

Vol 42 (11) ◽

pp. 1855-1860 ◽

Cited By ~ 54

Author(s):

F Muller ◽

M Dommergues ◽

L Bussières ◽

S Lortat-Jacob ◽

C Loirat ◽

...

Keyword(s):

Renal Function ◽

Gestational Age ◽

Obstructive Uropathy ◽

Perinatal Care ◽

Reference Intervals ◽

Tubular Reabsorption ◽

Reasonable Approximation ◽

Fetal Urine ◽

Beta 2 ◽

Beta 2 Microglobulin

Abstract Evaluation of fetal renal function by analysis of fetal urine sampled in utero may improve perinatal care after a prenatal diagnosis of bilateral obstructive uropathy. We provide reference intervals for 10 fetal urinary compounds and examine their variation with gestational age. Forty-one fetuses with bilateral obstructive uropathy (urine sampled between 20 and 38 weeks of gestational age) had normal, healthy values for serum creatinine (< or = 50 mumol/L) at ages 1-2 years. These cases were thus assumed to represent a reasonable approximation to healthy values. Sodium and beta 2-microglobulin concentrations significantly decreased with gestational age; calcium, ammonia, and creatinine significantly increased; glucose, phosphorus, chloride, urea, and total protein concentrations did not vary. Our results provide reference values for prenatal evaluation of fetal renal function and suggest that glomerular filtration of macromolecules and tubular reabsorption of glucose and phosphorus are mature by 20 weeks of gestation, whereas tubular reabsorption of sodium and beta 2-microglobulin increases progressively during the second half of gestation.

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